Storage Buffer | PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Peptide Affinity Purified |
Clonality | Polyclonal |
Specificity | Detects ~44 kDa. |
Cite This Product | Rabbit Anti-Human ERK1 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1142) |
Certificate of Analysis | A 1:250 dilution of SPC-1142 was sufficient for detection of ERK1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19123655
Alternative Names | ERT2 Antibody, Extracellular Signal Regulated Kinase 1 Antibody, HGNC6877 Antibody, HS44KDAP Antibody, HUMKER1A Antibody, Insulin Stimulated MAP2 Kinase Antibody, MAP kinase 1 Antibody, MAP kinase 3 Antibody, MAP Kinase Antibody, MAP kinase isoform p44 Antibody, ERK1 Antibody, MAPK 1 Antibody, MAPK 3 Antibody, MAPK Antibody, MAPK1 Antibody, MGC20180 Antibody, Microtubule Associated Protein 2 Kinase Antibody, Mitogen Activated Protein Kinase 3 Antibody, MK03_HUMAN Antibody, OTTHUMP00000174538 Antibody, OTTHUMP00000174541 Antibody, p44 ERK1 Antibody, p44 MAPK Antibody, P44ERK1 Antibody, PRKM 3 Antibody, PRKM3 Antibody, Protein Kinase Mitogen Activated 3 Antibody |
Cellular Localization | Cytoplasmic |
Accession Number | NP_002737 |
Gene ID | 5595 |
Swiss Prot | P27361 |
Scientific Background | ERK1 or MAPK3 is ubiquitously expressed protein serine/threonine kinase involved in regulating cellular differentiation and proliferation in response to extracellular mitogens or growth factors. MAPK3 is activated by upstream signalling by monomeric G-proteins. MAPK3 activation leads to its translocation from the cytosol to the nucleus where it can target substrate proteins involved in cell cycle regulation; Cyclin D1, suppression of apoptosis; BCL2 and BAD, tumour supression; p53, cell migration; PAI-1, and MHCII mediated immune response; PPARg1. Dysfunction of MAPK3 expression and activity has been observed in a variety of cancers. Predominantly overexpression of MAPK3 or constitutive activation has been observed in cancer cell lines. Activation of MAPK3 does not appear to be due to mutations in the protein itself but rather aberrant signalling in upstream factors including Raf, Ras and epidermal growth factor receptor. MAPK3 may also have a role in promoting metastasis in invasive tumours. |
Product Name | ERK1 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description |
Rabbit Anti-Human ERK1 Polyclonal |
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Species Reactivity | Human, Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications |
, WB , AM |
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Antibody Dilution | WB (1:250); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic peptide of human ERK1 (AA 23-37) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates | Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
AP (Alkaline Phosphatase)Properties:
HRP (Horseradish peroxidase)Properties:
Biotin
Properties: StreptavidinProperties:
|
Storage Buffer | PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Peptide Affinity Purified |
Clonality | Polyclonal |
Specificity | Detects ~44 kDa. |
Cite This Product | Rabbit Anti-Human ERK1 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1141) |
Certificate of Analysis | A 1:250 dilution of SPC-1141 was sufficient for detection of ERK1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19123650
Alternative Names | ERT2 Antibody, Extracellular Signal Regulated Kinase 1 Antibody, HGNC6877 Antibody, HS44KDAP Antibody, HUMKER1A Antibody, Insulin Stimulated MAP2 Kinase Antibody, MAP kinase 1 Antibody, MAP kinase 3 Antibody, MAP Kinase Antibody, MAP kinase isoform p44 Antibody, ERK1 Antibody, MAPK 1 Antibody, MAPK 3 Antibody, MAPK Antibody, MAPK1 Antibody, MGC20180 Antibody, Microtubule Associated Protein 2 Kinase Antibody, Mitogen Activated Protein Kinase 3 Antibody, MK03_HUMAN Antibody, OTTHUMP00000174538 Antibody, OTTHUMP00000174541 Antibody, p44 ERK1 Antibody, p44 MAPK Antibody, P44ERK1 Antibody, PRKM 3 Antibody, PRKM3 Antibody, Protein Kinase Mitogen Activated 3 Antibody |
Cellular Localization | Cytoplasmic |
Accession Number | NP_002737 |
Gene ID | 5595 |
Swiss Prot | P27361 |
Scientific Background | ERK1 or MAPK3 is ubiquitously expressed protein serine/threonine kinase involved in regulating cellular differentiation and proliferation in response to extracellular mitogens or growth factors. MAPK3 is activated by upstream signalling by monomeric G-proteins. MAPK3 activation leads to its translocation from the cytosol to the nucleus where it can target substrate proteins involved in cell cycle regulation; Cyclin D1, suppression of apoptosis; BCL2 and BAD, tumour supression; p53, cell migration; PAI-1, and MHCII mediated immune response; PPARg1. Dysfunction of MAPK3 expression and activity has been observed in a variety of cancers. Predominantly overexpression of MAPK3 or constitutive activation has been observed in cancer cell lines. Activation of MAPK3 does not appear to be due to mutations in the protein itself but rather aberrant signalling in upstream factors including Raf, Ras and epidermal growth factor receptor. MAPK3 may also have a role in promoting metastasis in invasive tumours. |