Rom the HisTrap column applying IMAC buffer containing 50 mM imidazole. Determined by the chromatogram, the collected hGCSF was analyzed by 10% Tristricine SDS-PAGE. Supplies and Approaches Building of plasmids and expression in E. coli The hGCSF gene encodes a protein comprising 204 amino acids, the very first 29 of which kind the signal peptide. …
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