HiPS cells may well represent new harmless instrument for tissue fix alternate to whole-cell therapies in vivo. Funding: This review was funded by NCN and NCBR grants: SONATA BIS-3 (UMO-2013/10/E/NZ3/ 007500) and STRATEGMED III (STRATEGMED3/ 303570/7/NCBR/2017) to EZS and PRELUDIUM-11 (UMO-2016/21/N/NZ3/00363) to KKW.PS03.Cardioprotective and proangiogenic probable of little extracellular vesicles secreted from amniotic fluid stem cells Kaloyan Takova, Filipa Vlahovab, Pascale Guillotb, Derek Yellona and Sean Davidsonaa The Hatter Cardiovascular Institute, University School London, London, United Siglec-5/CD170 Proteins Purity & Documentation kingdom; bInstitute for Women’s Vitamin D Receptor Proteins Biological Activity Wellbeing, University University London, London, UKinvestigated (making use of Boyden’s Chamber assay, MTT assay and western blot analysis/phosphokinase arrays, respectively). Outcomes: Isolated AFSC sEVs have been CD9/CD63/CD81positive and of substantial purity (as much as one.2×10^10 particles/ protein). These vesicles were not cardioprotective in models of simulated ischaemia/reperfusion damage in major cardiomyocytes in vitro. Nonetheless, AFSC sEVs carried promigratory cytokines and angiogenic variables (e.g. SDF-1, MIF, PTX3) and promoted endothelial cell migration and proliferation in vitro. Pharmacological inhibition of PI3K (a promigratory signalling pathway) in target endothelial cells decreased sEV-stimulated migration by 54 15 (p 0.001). Even so, sEVs did not induce phosphorylation of downstream PI3K targets, indicating that sEV effects may possibly be multifactorial and may perhaps involve several pathways. Summary/Conclusion: AFSC sEVs did not have direct protective results on cardiomyocytes in vitro but possessed proangiogenic prospective which calls for, but just isn’t solely dependent on, PI3K signalling. Ongoing experiments involve analyses in the sEV proteome, their cardioprotective properties in a model of rat myocardial ischaemia/reperfusion damage in vivo and their position in capillary sprouting from rat aortic explants. With each other, these information will define the likely for making use of AFSC sEVs as cardioprotective and proangiogenic treatment. Funding: BHFPS03.CystatinC and CD14 in plasma extracellular vesicles are linked with each renal dysfunction and heart failure in individuals presenting with dyspnoea Mirthe Dekkera, Farahnaz Waissib, Laura Verbree, Irwani Ibrahim, Shirley Ooi, Jiong-Wei Wangc, Win Kuand, Siew Chanc, Linda Peelene, Diederick Grobbee, A. Mark Richards, Carolyn Lam, Ya-Nan Zhang, Muhammad I Mazlan, Dominique de Kleijnfa cIntroduction: Mesenchymal stem cells (MSCs) exhibit antiapoptotic and proangiogenic functions in models of myocardial infarction, a typical reason behind death and disability. These effects are partially mediated by secreted little extracellular vesicles (sEVs). Amniotic fluid stem cells (AFSCs) are foetal MSCs with superior practical potential to grownup MSCs. We hypothesized that sEVs released by AFSCs are cardioprotective and proangiogenic. Approaches: Human AFSC sEVs have been isolated from serum-free conditioned medium by size-exclusion chromatography and characterized employing nanoparticle monitoring, dot blots, protein and immunoassays, electron microscopy and protein arrays. Their cardioprotective prospective was examined in versions of hypoxia/reoxygenation- and reactive oxygen species-induced death of principal adult rat cardiomyocytes in vitro. AFSC sEV results on human endothelial cell migration, proliferation and signalling pathway activation were alsoUMC Utrecht, Utrecht, Netherlands; bUMC Utrecht, Utrecht, Netherlands; National University of Singapore,.