Rket. Nevertheless, with such fantastic power comes terrific responsibility to appropriately prepare the instrument and samples for effective nanoscale flow cytometry experiments. The CytoFLEX is for Investigation Use Only. Individual results might vary. The Beckman Coulter item and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. inside the USA as well as other countries.PF06.Improved scatter sensitivity of a flow cytometer for detection of extracellular vesicles Leonie de Ronda, Edwin van der Polb, Ludovic Monheimc, Ton van Leeuwend and Frank Coumansea Amsterdam University Healthcare Centers, Amsterdam, USA; bAmsterdam UMC, University of Amsterdam, Department of Biomedical Engineering and Physics, Amsterdam, Netherlands; cBD Life Sciences, Erembodegem, Belgium; ddAmsterdam UMC, University of Amsterdam, Department of Biomedical Engineering and Physics, Amsterdam, Netherlands, ; e Amsterdam UMC, University of Amsterdam, Laboratory of Experimental Clinical Chemistry, Amsterdam, Netherlands,PF06.Preparing a CytoFLEX for Nanoscale flow Cytometry George Brittain, Sergei Gulnik and Yong Chen Beckman Coulter Life Sciences, Miami, USAIntroduction: Built about semiconductor technology, with a number of innovations to boost light capture, decrease noise and avert signal losses, the CytoFLEX is capable of CD49d/Integrin alpha 4 Proteins custom synthesis detecting biological nanoparticles (NPs) as compact as 80 nm by light scatter, and has a linear fluorescence variety that extends down into the single digits for fluorophores like FITC. Having said that, in order to effectively setup the CytoFLEX for NP analyses, a number of considerations need to be taken into account, some of which are extraordinary to standard flow cytometry. Solutions: In this poster, we are going to demonstrate ways to adequately setup and clean a CytoFLEX flow cytometer for NP analyses. First, we will explore the unique threshold selections and sensitivity ranges. Next, we will show how to clean the instrument and lessen noise. And ultimately, we will go over several vital difficulties that impact appropriate sample analyses. Final results: The 3 principal detection strategies on the CytoFLEX are FSC, SSC and Violet-SSC (VSSC). FSC on the CytoFLEX utilizes comparative signal analyses in lieu of traditional small-angle scatter, and is accurate for sizing events from 500 nm to 50 , independent in the refractive index or membrane integrity. The biological threshold sensitivities for SSC and VSSC around the CytoFLEX variety roughly among 250 nm0 and 80 nm , respectively. So that you can take full advantage with the reduce end of those scatter ranges, cleaning the instrument and thoughtful sample preparation are very crucial. Summary/Conclusion: Eventually, the CytoFLEX is amongst the most sensitive flow cytometers on theIntroduction: To investigate the biomarker possible of extracellular vesicles (EVs), EV subtypes are studied by flow cytometry. A flow cytometer VISTA Proteins supplier detects fluorescence, forward (FSC) and side scattered (SSC) light of single EVs. Even so, the scatter intensities in the majority of EVs are below the detection limit of widespread flow cytometers because EVs are small and possess a low refractive index. We aim to improve the scatter sensitivity of a widespread flow cytometer 450-fold for SSC and 107-fold for FSC, which will permit detection of one hundred nm EVs. Improved scatter sensitivity enables us to derive the size of EVs in the scatter signal and to boost the fraction of EVs which will be characterized working with immunofluorescence as well as scatter-based sizi.