Atelets differed notably from one another whereas the EVs resembled the entire solution. The GPL profile of platelets was additional prone to time-dependent alterations than that in the complete product and EVs. Clear time-dependent differences inside the pro-resolving and pro-inflammatory LMs with the entire product and EVs were observed. The enzymes needed for the production of LMs were also present within the concentrate. Summary/Conclusion: During platelet storage time-dependent alterations in LM and GPL profiles alter platelet functionality. Further analyses is going to be necessary to allow tailoring of concentrate use to avoid ATR and to optimize patient suitability. Funding: Part of this function was VRK Serine/Threonine Kinase 1 Proteins Storage & Stability funded by Tekes programs SalWe GID and NanoSkin, the Academy of Finland and Magnus Ehrnrooth Foundation.Background: EVs are involved in cellular communication and they could serve as effective carriers to provide chemotherapeutic drugs to tumor cells, but the biodistribution of exogenous EVs is determined by cell source, purification techniques and artificial targeting. Anyhow purification and characterization stay challenging for many factors for instance i) lack in standardization methods, ii) high variability of EVs production, moreover the composition of EVs can transform according to the time and conservation system The aim with the present study was to locate an univocal and reproducible process to acquire pure EVs. Methods: We compared and combined different purification approaches such as: ultra-filtration (UF), differential centrifugation (DC), density gradient centrifugation (G), size exclusion chromatography (SEC) and salting out (SO). Then we analyze the resulting suspension focusing mainly in tow aspect: particle size distribution and Protein- Lipid ratio. Pericles size distribution has been analyzed with the NTA as well as the asymmetrical-flow field-flow fractionation (AF4) coupled to a multiangle light-scattering detector (MALS) which is thought of the golden regular with regards to the EVs purifications. Protein- lipids ratio has been studied having a bio-photonic method, we applied the as Infrared and Raman spectroscopy, both are vibrational spectroscopy method and they may be complementary each and every other’s. Benefits: The combination of different purification procedures (DC + G) permits to obtain samples using a low concentrations of no cost proteins and aggregate. The particle size distribution seems not impacted by distinct protocols that implies we’re in a position to recovery each small exosomes and big microvesicles right after every single purification steps. The spectra obtained with Raman and IR spectroscopy shown peak related to diverse chemical entities as amide and alkyl group. The protein- lipids ratio is determinate by the comparison on the intensity of this two peak. Our outcomes underline that the multi methods purification protocols is able to reach a decrease P/L that implies the samples consists of a decrease quantity of free of charge proteins. Summary/Conclusion: In accordance with our information the combinations of two unique strategies, for Serine Carboxypeptidase 1 Proteins MedChemExpress example UF or DC with the density gradient gentrification leads to a far more pure samples that shown ales volume of aggregates, more uniform particle size distribution plus a decrease protein-lipids ratio.ISEV 2018 abstract bookSS 30 LB: Symposium Session 30 Late Breaking Abstracts Chair: Lei Zheng Place: Room 6 09:000:LB03.Regulation of exosome secretion by means of the intricate tuning of multivesicular endosome transport towards the plasma membrane Maarten P. Bebelman1; Frederik Verweij2; Roberta Palmulli3; Xavier Heili.