Otillin-1 and Alix. As outlined by the NTA the EVs were heterogeneous in size. Summary/Conclusion: HOK-16B cells released EVs that have common EV markers. The EVs derived from HOK-16B infected with periodontopathogen have to analyse and confirm the biological function to other cells. Funding: This operate was supported by National Research Foundation of Korea grants (No. NRF-2018R1A5A2 024418 and NRF-2018R1A2A2A05018558).PF01.Air pollution effects on the clinical course of autoimmune diseases: the role of extracellular vesicles Mirjam Hoxhaa, Tommaso Schioppob, Simona Iodicea, Laura Pergolia, Nicola Ughib, Luca Ferraria, Francesca Ingegnolib and Valentina BollatiaaUniversity of Milan, Division of Clinical Sciences and Neighborhood Overall health, Milan, Italy; bDivision of Clinical Rheumatology, G. Pini Hospital, Milano, ItalyPF01.Isolation of EVs derived from human oral keratinocytes Younggap Lim and Bong-Kyu Choi Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, Jongno-gu, Seoul, Fc gamma RII/CD32 Proteins Recombinant Proteins Republic of Korea, Seoul, Republic of KoreaIntroduction: Oral keratinocytes would be the initially defense line against external environments such as chemical agents, microbes and physical factors. Stimulated oral keratinocytes make cytokines/chemokines to modulate nearby inflammatory status. Depending on current researches, not only cytokines/chemokines but extracellular vesicles (EVs) also regulate immune response. Hence, we hypothesized that oral keratinocytes release EVs and these EVs could modulate immune response in the gingival tissue. Solutions: EVs have been isolated from human oral keratinocytes (HOK-16B) by ultracentrifugation (UC) and commercial EVs isolation kit and analysed by western blotting and Nanoparticle Tracking Analysis (NTA). Benefits: To exclude EVs originated from cell culture medium, we compared three diverse keratinocyte culture media, then we chose medium that contained theIntroduction: Autoimmune illnesses (Ads) are characterized by the body’s intolerance to self-antigens. The cause of autoimmunity continues to be unknown. However, it really is generally accepted that Ads could possibly be triggered by environmental aspects capable to increase inflammation. In current years, extracellular vescicles (EVs) have been described to play a vital function both in Ads pathogenesis and environmental toxicants, including particulate matter (PM). The aim of our study is always to evaluate PM effects on EV release in Advertisements. Solutions: We recruited 24 patients with Ads (12 Rheumathoid Arthritis, RA and 12 Systemic Sclerosis, SSc) and 12 individuals with Osteoarthritis (OA), a nonautoimmune inflammatory illness taken as handle. Plasma EVs have been analysed by Nanosight and flow cytometry right after labelling using the following markers: CD14+ (monocyte), CD61+ (platelet), CD25+ (T-reg), ERVWE1+ (human Natriuretic Peptide Receptor B (NPR2) Proteins manufacturer endogenous retrovirus W), HLAG + (human leukocyte antigen G). PM10 and PM2.5 concentrations at the residency of each and every topic had been obtained from the regional air quality monitoring network. Results: The increase of PM2.5 led to a lower of MVs CD14+ ( = -0.13; p 0.01) and CD61+ ( = -0.08; p = 0.05) in RA, of ERVWE1+ in each SSc ( = -0.10; p = 0.01) and OA ( = -0.09; p = 0.01), and of HLA+ ( = -0.12; p 0.01) only in SSc. Comparable benefits have been observed analyzing PM10 exposure. Analysis of EVs concentration according to theirISEV2019 ABSTRACT BOOKdimensions showed a negative association inside the size selection of exosomes (632 nm) in RA and SSc compared to OA (p 0.05). Lastly, we obse.