Host:pathogen interaction will probably be discussed. Funding: This function was funded by the National Institutes of Health, USA.Final results: Quantitative image analysis showed that NRBC and each nEVs and pEVs triggered modulation of VE-cadherin expression, whereas PRBC and PRBC-Mix circumstances resulted within a considerable down-regulation. We also observed that p-EVs were taken up by HBEC at twice the price of nEVs. Expression of eCAMs, was increased inside the presence of PRBCs and additional improved with PRBC-Mix. Summary/Conclusion: These benefits recommend that Caspase 4 Inhibitor Purity & Documentation interactions amongst EVs and their cells of origin usually do not always trigger precisely the same cellular response in their target cell. Thus, the combined presence of both EVs and cells might either potentiate or compensate every single other effects. Further studies are necessary to decide which molecular pathways are involved inside the modifications observed. Funding: This function was funded by the University of Technologies Sydney (internal funds) along with the Australian National Wellness Healthcare Investigation Council Project Grant.OS22.Exploration of extracellular vesicles from Ascaris suum delivers evidence of parasite-host cross speak Eline P Hansen1; Bastian Fromm2; Sidsel D Andersen3; Antonio Marcilla4; Kasper L Andersen1; Andrew R Williams1; Aaron R Jex5; Robin B Gasser6; Neil D Young6; Ross S Hall6; Allan Stensballe7; Yan Yan8; Merete Fredholm1; Stig M Thamsborg9; Peter Nejsum10 Department of Veterinary and Animal Sciences, Faculty of Well being and Medical Sciences, University of Copenhagen, Denmark, Copenhagen, Denmark; 2Department of Tumor Peter Nejsum Biology, Institute for Cancer Study, The Norwegian Radium Hospital, Oslo University Hospital, Norway, Oslo, Norway; 3Department of Clinical Medicine, Faculty of Wellness, Aarhus University, Denmark, Aarhus, Denmark; 4Departament de Farm ia I Tecnologia Farmac tica i Parasitologia, Universitat de Val cia, Spain, BURJASSOT (VALENCIA), Spain; 5Population Overall health and Immunity Division, The Walter and Eliza Hall Institute, Australia, Melbourne, Australia; 6Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Australia, Melbourne, Australia; 7Department of Health Science and Technology, Aalborg University, Denmark, Aalborg, Denmark; 8 Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark, Aarhus, Denmark; 9Department of Veterinary and Animal Sciences, Faculty of Wellness and Healthcare Sciences, University of Copenhagen, Denmark, Melbourne, Australia; 10Aarhus University, Denmark, Aarhus N, DenmarkOS22.The role of extracellular vesicles within the modulation of endothelial junctions in an in vitro model of cerebral malaria Valery Combes; Benjamin Sealy; Iris Cheng The University of Technologies Sydney, Sydney, AustraliaBackground: Malaria resulted in 438,000 deaths in 2015, with 90 due to cerebral malaria (CM). CM happens when Plasmodium falciparuminfected red blood cells (PRBCs) sequestrate within the cerebral microvasculature causing neurological lesions associated with alteration with the blood rain barrier (BBB). Applying in vitro c-culture systems and murine models, recent research by our group and others have recommended that extracellular vesicles (EVs) participate towards the improvement in the vascular lesion for the duration of CM. Approaches: Applying an in vitro BBB model, we aim to investigate the Caspase 3 Chemical Purity & Documentation impact that EVs have on the modulation of endothelial integrity by measuring the expression of VE-cadherin plus the activation status in the endothelial monolayer. EVs released by each nor.