Usion: Medin and MFGE8 are abundant in aged subjects and are secreted by exosomes in to the ECM. Exosome release is increased with age, which could contribute towards the deposition of medin within the ECM along with the formation of amyloid. MFGE8 may well play a part in accelerating calcification by inducing an osteogenic phenotype through the ERK pathway. Both MFGE8 and medin secretion by exosomes could contribute towards the age-related development of vascular calcification. Funding: This work is funded by the British Heart Foundation.applied as cellular ageing model. Dx accelerated ageing, but Wnt4-containing exosomes could effectively counteract Dx-induced senescence. We have obtained diverse staining patterns working with DiI-labelled Wn4-exosomes on sections of young and aged samples. Ultimately, in vivo injected DiI-labelled Wnt4-exosomes showed detectable homing to the thymus. Summary/Conclusion: Based on our final results Wnt4 and miR27b are present in TEC exosomes. Our findings indicate that Wnt4 is usually a crucial inhibitor thymic involution potentially by way of miR27b. Having said that, further experiments are needed for probable applications. Funding: Scientific analysis help was Cathepsin L Inhibitor Molecular Weight supplied by PTE AOK KA2016-16, PTE Pharmaceutical Talent Center plan and the PTE Viral Pathogenesis Talent Center plan by means of KK. The Janos Bolyai Scholarship with the Hungarian Academy of Sciences also supported KK.PS06.Extracellular vesicles and their miRNA cargo in ageing and ageassociated diseases Lucia Terlecki-Zaniewicz1; Vera Pils1; Ingo L mermann1; Regina Weinm lner1; Madhusudan Bobbili Reddy1; Markus Schosserer1; Florian Gruber2; Matthias Hackl3; Johannes Grillari1 CDL for Biotechnology of Skin Aging BOKU Department of Biotechnology, Vienna, Austria; 2Department of Dermatology, Healthcare University of Vienna, Austria; Christian Doppler Laboratory for the Biotechnology of Skin Aging, Vienna, Austria, Vienna, Austria; 3TAmiRNA GmbH Vienna, Vienna, AustriaPS06.11 = OWP1.Part of Wnt4 exosomes in thymic ageing Krisztina Banfai1; Kitti Garai1; David Ernszt2; Judit E. Pongracz1; Krisztian KvellInstitute of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, Pecs, Hungary, P s, Hungary; 2Institute of Physiology, Faculty of CYP3 Inhibitor Compound Medicine, University of Pecs, Pecs, Hungary, P s, HungaryBackground: Wnt4 plays a essential role in promoting the development and halting the ageing with the thymus. Through ageing Wnt4 is downregulated, although PPAR is up-regulated and triggers adipose involution. However, miR27b was described to suppress PPAR. Our objective was to prove the presence of Wnt4 in exosomes, to detect its impact and stick to its path both in vitro and in vivo. Techniques: Exosomes have been harvested from control and Wnt4 overexpressing TECs (thymic epithelial cells) for further experiments. Exosomes had been visualized by transmission electron microscopy. Exosomal miR27b levels had been measured by TaqMan qPCR, though Wnt4 protein content was assayed by ELISA. DiI-labelled exosomes were applied on mouse and human thymus sections and also iv-injected into mouse for in vivo tracking. Results: Transmission electron microscopy showed exosomes ranging 50100 nm in size. TaqMan miRNA assay measured elevated miR27b levels, when ELISA showed higher Wnt4-content in Wnt4-exosomes when compared with manage exosomes. For functional research steroid (Dx)-induced TECs wereBackground: Cellular senescence has evolved from an in vitro model technique to study ageing to a multifaceted phenomenon of in vivo importance as senescent cell removal delays t.