On NextSeq Higher Output single-end sequencing run. Benefits: Administration of AFSC-EVs enhanced terminal bud density and surface area of lung explants back to handle levels and promoted lung epithelial cell differentiation in lung organoids (elevated SPC andPF12.10=OWP2.HIV-specific antibody-mediated targeting of ENV+ tissues by exosomes Zou Xue, Yuan M’eng, Zheng Nan and Wu Zhiwei Nanjing University, Nanjing, China (People’s Republic)Introduction: ART (Antiretroviral Therapy) can effectively suppress HIV replication in the peripheral blood to an undetectable level. On the other hand, efforts to eradicate the latent virus in reservoirs remain a challenge and are a major obstacle inside the treatment of HIV patients. Exosomes exhibit substantial guarantee as an endogenous drug delivery nanosystem for delivering drugs to reservoir tissues provided their special properties, like low immunogenicity, innate stability, high delivery efficiency and mainly importantly the capability to penetrate strong tissues as a consequence of their lipophilic properties. Approaches: Within this study, we engineered and expressed the ScFv of a high affinity HIV-specific monoclonal antibody, 10E8, on exosome surface. Exosomes from 293T cells have been loaded with curcumin by means of saponin, with effective up to 34 . 10E8ScFv-expressing exosomes (10E8-Exo) showed highly effective targeting of and curcumin delivery to CHO cell that expresses a trimeric gp140 on its surface (ENV+ cells) in vitro as demonstrated by confocal imaging and flow cytometry. We showed that 10E8-Exo could correctly bind to CHO cell that expresses a trimeric gp140 on its surface. The exosomes loaded with curcumin, a chemical that was shown to kill HIV-infected cells, showed certain killing on the trimeric gp140-expressing CHO cells. In an NCG mouse model that was grafted using the tumourigenic gp140-CHO cells and MNK Purity & Documentation created strong tissue tumours intravenously injected 10E8-Exo targeted the ENV-expressing tissues and delivered curcumin to induce a strong suppression in the ENV+ tumour growth having a low toxicity. Benefits: Our benefits demonstrated that engineered exosomes can deliver anti-HIV agents to strong tissues byISEV2019 ABSTRACT 5-HT4 Receptor Antagonist site BOOKspecifically targeting cells expressing viral ENV and induce cell killings. Summary/conclusion: It suggesting that such an strategy could be created for eradicating virusinfected cells in tissue reservoir Funding: This study was supported by The National Crucial Study and Development Program of China(2016YFC1201000), Nature Science Foundation of Jiangsu Province (BY2015069-02) and National Nature Science Foundation of China (81672020). The funders had no role in study design, data collection and evaluation, selection to publish, or preparation of the manuscript.JOURNAL OF EXTRACELLULAR VESICLESLate breaking- EVs and cancer Chairs: Sonia Melo; Golnaz Morad Place: Level three, Hall A 15:306:LBF01.Exosomes from LNCaP cells market the activity of osteoblasts through the transfer of mir-375 Yun Yea and Su-liang Liba Prostate Cancer, Xi’an, China (People’s Republic); bCancer, Xi’an, China (People’s Republic)for Cancer Research, Tokyo, Japan; cCancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Study, Tokyo, JapanIntroduction: Studies have shown that exosomes influence tumour metastasis, diagnosis and therapy. It has been located that exosomal miRNAs are closely linked for the metastatic microenvironment. Nonetheless, the regulatory role of exosomes from prostate cancer (PCa) cells in.