Rabbit anti-Nurr1 Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Synonym Nur-ralated factor 1; Nuclear receptor-related factor 1; Orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; Regenerating liver nuclear receptor 1; RNR-1; SL-322; Nuclear orphan receptor HZF-3; T-cell nuclear receptor NOT; HZF 3; HZF3; Immediate-early response protein NOT; Intermediate early receptor protein; NGFI B/nur77 beta type transcription factor homolog; NOT; NR4A2; NR4A2_HUMAN; Nuclear receptor subfamily 4 group A member 2; Nur related protein 1 homolog; Nurr 1; RNR 1; RNR1; TINUR; Transcriptionally inducible nuclear receptor; Transcriptionally inducible nuclear receptor related; Transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor |Host Rabbit |Specificity Nurr1 |Species Reactivity Human, Rat |Predicted Reactivity Mouse, Pig, Cow, Rabbit |Applications WB=1:500-2000 |Immunogen KLH conjugated synthetic peptide derived from human Nurr1:301-400/598 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Chromatin and nuclear signaling Neurobiology & have spent Metabolism Epigenetics |Target |Background Nuclear receptor and transcription factor; plays a role in development and maintenance of neurons synthesizing the neurotransmitter dopamine This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full length nature has not been determined. |Cellular localization Cell nucleus; |UniProt P43354 | Sample: Raji Cell (Human) Lysate at 40 ugPrimary: Anti- Nurr1 (abs119757) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 67 kDObserved band size: 65 kD| Sample: MCF-7 Cell (Human) Lysate at 40 ugHepG2 Cell (Human) Lysate at 40 ugPrimary: Anti- Nurr1 (abs119757) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 67 kDObserved band size: 70 kD| Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (Nurr1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.