Versity in gut microbiota in comparison to the model group.BT 1 BT two O T1 O T2 D T1 D TFoods 2021, ten,12 ofTable two. The effects of teas on gut microbial community richness and diversity. mTOR Modulator web groups CTRL EtOH BT1 BT2 OT1 OT2 DT1 DT2 Chao 1 11.33 1.70 27.83 two.62 18.97 three.64 19.23 5.26 16.83 two.49 # 17.83 1.55 # 17.03 2.90 # 12.17 2.72 ### Simpson 0.23 0.02 0.12 0.00 0.14 0.02 0.13 0.01 0.21 0.02 ## 0.22 0.03 ## 0.23 0.04 ### 0.32 0.02 ### Shannon 0.78 0.04 1.02 0.01 1.02 0.03 0.97 0.04 0.95 0.02 0.87 0.05 ## 0.91 0.04 # 0.86 0.04 ##Note: CTRL, the control group; EtOH, the model group; BT1, Dianhong Tea; BT2, Yingde Black Tea; OT1, Tieguanyin Tea; OT2, Fenghuang Danzong Tea; DT1, Fu Brick Tea; DT2, Selenium-Enriched Dark Tea. Chao 1 reflects community richness of intestinal flora; Simpson and Shannon reflect community diversity of intestinal flora. p 0.001, the model group compared with all the control group; # p 0.05, ## p 0.01, ### p 0.001, the tea extract supplementary groups compared with the model group.Subsequently, to investigate the similarities in the structure and composition on the gut microbial community among various fecal samples or groups, beta-diversity evaluation and species RIPK2 Inhibitor Compound abundance cluster analysis had been carried out, respectively. Seen from Figure 7A, beta-diversity analysis depending on PCoA evaluation indicated that the microbial structure was remarkably disturbed by chronic alcohol exposure when compared with the handle group, however the influence may be inhibited and remedied to typical status by means of the treatment of Tieguanyin Tea (OT1), Fenghuang Danzong Tea (OT2), Fu Brick Tea (DT1), and SeleniumEnriched Dark Tea (DT2) extracts. Even so, the microbial structures within the fecal 25 samples of Foods 2021, ten, x FOR PEER Critique 13 of Dianhong Tea (BT1) and Yingde Black Tea (BT2) groups have been in accordance together with the model group, and also the 3 groups had been clearly separated from other groups along PCoA1 (38.6 ). It was also indicated that Dianhong Tea (BT1) and Yingde Black structure triggered by didn’t Tea (BT2) extracts did not substantially inhibit the transform of microbial Tea (BT2) extracts significantly inhibit the alter of microbial structure triggered by chronic alcohol intake. chronic alcohol intake.Figure 7.Figure 7. The effects ofon the structure ofof microbiota in mice impacted by chronic alcohol exposure. (A) PCoA evaluation. analysis. The effects of teas teas around the structure microbiota in mice affected by chronic alcohol exposure. (A) PCoA (B) species abundance cluster analysis in the phylum level. (C) species abundance cluster evaluation in the genus level. CTRL, (B) species abundance cluster evaluation in the phylum level. Tea; species abundance clusterTieguanyin at the genus level. the handle group; EtOH, the model group; BT1, Dianhong (C) BT2, Yingde Black Tea; OT1, evaluation Tea; OT2, Fenghuang Danzong Tea; DT1, model group; BT1, Dianhong Tea; BT2, Yingde Black Tea; OT1, Tieguanyin Tea; OT2, CTRL, the handle group; EtOH, theFu Brick Tea; DT2, Selenium-Enriched Dark Tea. Fenghuang Danzong Tea; DT1, Fu Brick Tea; DT2, Selenium-Enriched Dark Tea.As expected, the results of species abundance cluster evaluation determined by both phylum and genus levels showed that overt alter in the composition of gut microbiota was observed within the model group compared together with the control group as displayed in Figure 7B,C. Furthermore, Tieguanyin Tea (OT1), Fenghuang Danzong Tea (OT2), Fu Brick Tea (DT1), Selenium-Enriched Dark Tea (DT2), and control groups had sim.