Ipponense was significantly greater than that in the other developmental stages
Ipponense was substantially greater than that in the other developmental stages (Figure 7); this showed that MnFtz-f1 may possibly play a vital role within the procedure of oocyte mitosis. A recent study in Drosophila revealed that Drosophila oocytes couldn’t undergo standard mitosis within the absence of Ftz-f1, suggesting that Ftz-f1 was crucial for oocyte division (57). In Drosophila, Ftz-f1 is divided into two subtypes: aFtz-f1 and bFtz-f1. The aFtz-f1 is mainly expressed within the early stage of embryogenesis, even though bFtz-f1 is expressed inside the late embryonic stage and pupal stage (58). In theFrontiers in Endocrinology | www.frontiersinDecember 2021 | Volume 12 | ArticleYuan et al.Identification S1PR1 Accession Functions of MnFtz-fFIGURE four | The 3D-structures of MnFtz-f1 and Ftz-f1 predicted by I-TASSER. (A) shows the 3D-structure of the MnFtz-f1 gene of M. nipponense. (B ) show the 3D-structures from the Ftz-f1 gene of P. vannamei, H. americanus and P. monodon, respectively. The DNA-binding domain is marked with a red circle.current study, MnFtz-f1 was extremely expressed inside the early stage of important embryogenesis (CS), on the 5th day immediately after hatching, and around the 5th day just after larvae (Figure 7). MnFtz-f1 might have a comparable function of aFtz-f1 and bFtz-f1 in the embryonic and hatching stages. Ftz-f1 is amongst the 20E responsive genes, plus the lower in 20E level induces a rise in bFtz-f1 expression level (15, 17, 59). Consistent with previous analysis, in vivo administration of 20E significantly inhibited the expression level of MnFtzf1 (Figure eight). RNAi causes post-transcriptional gene silencing via double-stranded RNA (dsRNA) (60). In M. nipponense, RNAi has been widely employed in gene function evaluation (41, 61, 62). Inside the present study, the expression of MnFtz-f1 in M. nipponense ovaries was substantially lowered by the in vivo administrationof dsRNA. To further study the mutual connection of regulation in between the genes, the expression levels of Mn-Spook, Phantom, and Vg have been determined after MnFtz-f1 knockdown. Spook and Phantom are critical members of the Halloween gene family members and regulate molting by catalyzing the conversion of cholesterol to 20E (3). Mn-Spook plays a pivotal function in the molting of M. nipponense by participating in 20E production (41). In JAK Formulation Schistocerca gregaria, silencing of Spook reduces ecdysteroid titer and results in delayed nymphal development and failure to molt. Phantom would be the enzyme essential by the prothoracic glands of Bombyx and Drosophila to synthesize ecdysteroid (63). In crustaceans, Vg supplies power for ovarian development, as well as the maturation of ovaries is dependent upon the synthesis and accumulation of Vg (64, 65). Generally, Mn-Spook, Phantom,Frontiers in Endocrinology | www.frontiersinDecember 2021 | Volume 12 | ArticleYuan et al.Identification Functions of MnFtz-fFIGURE five | Expression of MnFtz-f1 mRNA in various tissues of M. nipponense. O, ovary; H, heart; G, gill; E, eyestalk; He, hepatopancreas; M, muscle. Statistical analyses were performed by one-way ANOVA. Information (imply SEM, n = 6) had been expressed relative for the expression of the eukaryotic translation initiation issue 5A (EIF) gene. Bars with distinct letters indicate substantial differences (P 0.05).and Vg are closely associated towards the molting or ovarian development of crustaceans. Studying the regulatory relationship among MnFtz-f1 and these genes in M. nipponense is additional conducive to our understanding from the molting and ovarian development processes of M. nipponen.