Hilic fungus Rhizomucor pusillus. Planet J Microb Biot 2004, 20(1):10509. 30. Singh B, Kaur P, Satyanarayana T: Fungal phytases in ameliorating nutritional status of foods and combating environmental phosphorus pollution. In Microbes: Well being and Environment. Edited by Chauhan AK, Verma A. New Delhi, India: IK International Publishers; 2006:28926. 31. Lassen SF, Breinholt J, tergaard PR, Brugger R, Bischoff A, Wyss M, Fuglsang CC: Expression, gene cloning, and characterization of five novel phytases from 4 Basidiomycete Fungi: Peniophora lycii, Agrocybe pediades, Ceriporia sp., and Trametes pubescens. Appl Environ Microb 2001, 67(ten):4701707. 32. Maenz DD, Engele-Schaan CM, Newkirk RW, Classen HL: The impact of minerals and mineral chelators on the formation of phytase-resistant and phytase-susceptible types of phytic acid in answer and in a slurry of canola meal. Anim Feed Sci Tech 1999, 81(3):17792. 33. Oh BC, Choi WC, Park S, Kim YO, Oh TK: Biochemical properties and substrate specificities of alkaline and histidine acid phytases. Appl Microb Biotech 2004, 63(four):36272. 34. Wyss M, Brugger R, Kronenberger A, R y R, Fimbel R, Oesterhelt G, Lehmann M, van Loon APGM: Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. Appl Environ Microb 1999, 65(2):36773. 35. Cao L, Wang WM, Yang CT, Yang Y, Diana J, Yakupitiyage A, Luo Z, Li DP: Application of microbial phytase in fish feed. Enzyme Microb Tech 2007, 40(4):49707. 36. Kerovuo J, Lappalainen I, Reinikainen T: The metal dependence of Bacillus subtilis phytase. Biochem Biophy Res Comm 2000, 268(2):36569. 37. Kim DH, Oh BC, Choi WC, Lee JK, Oh TK: Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive. Biotech Lett 1999, 21(11):92527. 38. Boyce A, Walsh G: Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer. J Biotech 2007, 132(1):827. 39. Fujita J, Yamane YI, Fukuda H, Kizaki Y, Wakabayashi S, Shigeta S, Suzuki O, Ono K: Production and properties of phytase and acid phosphatase from a sake koji mold, Aspergillus oryzae. J Biosci Bioeng 2003, 95(four):34853. 40. Greiner R, Konietzny U: Phytase for food application. Meals Tech Biotech 2006, 44(2):12540. 41. Quan CS, Tian WJ, Fan SD, Kikuchi JI: Purification and properties of a low- molecular-weight phytase from Cladosporium sp. FP-1. J Biosci Bioeng 2004, 97(4):26066. 42. In MJ, Search engine marketing SW, Kim DC, Oh NS: Purification and biochemical properties of an extracellular acid phytase created by the Saccharomyces cerevisiae CY strain.Brincidofovir Course of action Biochem 2009, 44(1):12226.LCS-1 43.PMID:23833812 Yu P, Chen YR: Screening and identification of novel phytase-producing Bacillus strain from soil. J Chin Inst Food Sci Tech 2010, 10:11621. 44. Laemmli UK: Cleavage of structural proteins throughout the assembly on the head of bacteriophage T4. Nature 1970, 227:68085. 45. Engelen AJ, Vander HFC, Randsdorp PHG, Smit EL: Simple and speedy determination of phytase activity. J AOAC Int 1994, 77:76064.46. Bradford MM: A fast and sensitive technique for the quantization of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 1976, 72:24854. 47. Fernandez J, DeMott M, Atherton D, Mische SM: Internal protein sequence evaluation: enzymatic digestion for significantly less than ten micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal Biochem 1992, 201(two):25564.doi:10.1186/1472-6750-13-78 Cite this.