Tion was assessed by using several regression. We calculated geometric mean and its 95 self-confidence intervals (CIs) of homocysteine concentrations (nmol/mL) for each and every tertile of serum fatty acid. The confounding variables regarded as were age (y, continuous), sex, workplace [A (surveyed in July) or B (surveyed in November)], BMI (kg/m2, continuous), smoking status (non-smoker or current smoker), alcohol consumption (non-drinker, drinker consuming 20 g of ethanol/d, or drinker consuming 20 g of ethanol/d), occupational physical activity (sedentary operate or active work), non-occupational physical activity (0, 0 to five or 5 MET-h/wk), log-transformed serum folate (ng/mL, continuous), vitamin B6 intake (mg/1000 kcal, continuous) and log-transformed vitamin B12 intake (g/1000 kcal, continuous). The very first model was adjusted for age, sex and workplace (model 1); as well as the second model was additional adjusted for BMI, smoking, alcohol consumption, occupational physical activity, non-occupational physical activity, serum folate, vitamin B6 intake and vitamin B12 intake (model 2). We adjusted for serum folate alternatively of folate intake because inside the present population, serum folate had a higher impact on the adjustments in odds ratio than did dietary folate. We made use of dietary information on vitamin B6 and vitamin B12 since we’ve serum B6 data only for any subgroup on the study population and no information on serum vitamin B12 concentrations. There had been no statistically significant interactions by sex (p for interaction 0.1 for all fatty acids), and therefore final results have been presented for men and females combined. Two-sided P values less than 0.05 were regarded as statistically substantial. All analyses were performed working with STATA version 12.0 (STATA Corp., College Station, TX, USA).Variations in the proportions and means of potential confounding variables across tertile categories of n-3 PUFA and n-6 PUFA had been assessed by utilizing the Mantel-Haenszel chi-square test for categorical variables and linear regression evaluation for continuous variables, withResults Qualities of study subjects according to tertile category of serum n-3 and n-6 PUFA in CE are shown in Table 1. Participants with larger serum n-3 PUFA were older, a lot more probably to become male, smokers and heavy drinkers and had larger levels of BMI, serum folate and dietary vitamin B12 intake, compared with those with decrease n-3 PUFA. Participants with larger serum n-6 PUFA levels had been younger, less probably to be male, smokers and heavy drinkers, had reduce levels of BMI, nonoccupational physical activity and dietary vitamin B12 intake, but had greater concentrations of serum folate, compared with these with reduced n-6 PUFA.Sacubitril/Valsartan Equivalent associations had been observed when participants had been categorized in line with serum n-3 and n-6 PUFAs in phospholipids (information not shown).Spartalizumab Pearson correlation coefficient was calculated to assess correlation between each and every PUFA.PMID:23756629 In phospholipids,Kume et al. Nutrition Metabolism 2013, ten:41 http://www.nutritionandmetabolism/content/10/1/Page 4 ofTable 1 Qualities in the subjects in line with tertile of n-3 PUFA and n-6 PUFA in serum cholesterol estern-3 PUFA T1 (two.74)1 No. of subjects Age (y, mean SD) Sex (male, ) Workplace (A, )n-6 PUFA T3 (5.79) 165 47.7 9.8 68.5 29.7 23.0 3.two 32.7 58.two 83.six 30.three 0.001 0.001 0.75 0.01 0.02 0.001 0.12 0.60 0.002 P-trend2 T1 (58.36) 166 47.9 ten.1 79.5 29.five 23.5 3.5 43.4 64.five 81.9 36.eight T2 (62.58) 165 41.four ten.four 57.0 31.five 22.3 3.5 26.1 35.two 84.two 30.3 T3 (66.18) P-trend 165.