Product Name :
NEDD8 (human), (recombinant) (biotin conjugate)
Sequence:
Purity:
Molecular Weight:
~11kDa (based on Bt3-NEDD8 as predominant form present).
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Use/Stability :
Stable for at least 12 months after receipt when stored at -20°C.
Description:
Biotinylated NEDD8 is a useful reagent for studying NEDDylation in vitro, where it represents a readily detectable alternative to radio-iodinated NEDD8. Biotinylated NEDD8 is activated by NEDD8 E1, is a competent substrate for the NEDD8 E2 conjugating enzyme UbcH12 and can be conjugated to target proteins in the presence of a suitable NEDD8 E3 ligase.{{16980-89-5} site|{16980-89-5} Technical Information|{16980-89-5} In Vivo|{16980-89-5} supplier} The biotinyl moiety also provides a potential route to affinity purification of conjugated substrates (or thiol ester-linked enzymes) on avidin/streptavidin matrices.{{1219168-18-9} medchemexpress|{1219168-18-9} Technical Information|{1219168-18-9} In stock|{1219168-18-9} custom synthesis} NEDD8 contains 9 lysine residues, any/many of which are likely to be biotinylated in this product. These modification sites have not been mapped. Biotinylated NEDD8 is prepared from recombinant human His-tagged NEDD8 . Western blot of biotinylated-NEDD8 thioester assay. Bt-NEDD8 conjugated to UbcH12 in the presence of NEDD8 E1 .PMID:31334949 Bt-NEDD8 conjugated species detected with streptavidin-HRP. Western blot of biotinylated-NEDD8 thioester assay. Bt-NEDD8 conjugated to UbcH12 in the presence of NEDD8 E1 . Bt-NEDD8 conjugated species detected with streptavidin-HRP.
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Additional Information :
| Formulation Lyophilized. Contains PBS. | MW ~11kDa (based on Bt3-NEDD8 as predominant form present). | Reconstitution Reconstitute with sterile distilled water. | Source Produced in E. coli. | Technical Info / Product Notes Biotinylated mature protein for NEDD8 conjugation and NEDDylation pathway studies. Suggested uses: Ready detection of NEDD8 modification of specific proteins in vitro.Demonstration that novel proteins are potential targets for NEDDylation under in vitro conditions.Purification of NEDD8-conjugates via streptavidin affinity chromatography. | UniProt ID Q15843