Anti-AHA2 Antibody

Anti-AHA2 Antibody__Rabbit Anti-Human AHA2 Polyclonal AZD-9291

Product Name	AHA2 Antibody
Description	Rabbit Anti-Human AHA2 Polyclonal
Species Reactivity	Human, Mouse, Rat
Applications	, WB , ICC/IF
Antibody Dilution	WB (1:1000), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
Host Species	Rabbit
Immunogen Species	Human
Immunogen	Aha2
Concentration	1 mg/ml
Conjugates	Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated APC (Allophycocyanin) Overview: High quantum yield Large phycobiliprotein 6 chromophores per molecule Isolated from red algae Molecular Weight: 105 kDa   Optical Properties: λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5     ATTO 390 Overview: High fluorescence yield Large Stokes-shift (89 nm) Good photostability Moderately hydrophilic Good solubility in polar solvents Coumarin derivate, uncharged Low molar mass: 343.42 g/mol  ATTO 390 Datasheet Optical Properties: λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm     ATTO 488 Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation New dye with net charge of -1 Molar Mass: 804 g/mol     Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC    ATTO 565 Overview: High fluorescence yield High thermal and photostability Good solubility in polar solvents Excellent solubility in water Very little aggregation Rhodamine dye derivative Molar Mass: 611 g/mol   Optical Properties: λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC    ATTO 594 Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation New dye with net charge of -1 Molar Mass: 1137 g/mol   Optical Properties: λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red®    ATTO 633 Overview: High fluorescence yield High thermal and photostability Moderately hydrophilic Good solubility in polar solvents Stable at pH 4 – 11 Cationic dye, perchlorate salt Molar Mass: 652.2 g/mol Optical Properties: λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5    ATTO 655 Overview: High fluorescence yield High thermal and photostability Excellent ozone resistance Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 634 g/mol Optical Properties: λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5    ATTO 680 Overview: High fluorescence yield Excellent thermal and photostability Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 631 g/mol   Optical Properties: λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5    ATTO 700 Overview: High fluorescence yield Excellent thermal and photostability Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 575 g/mol   Optical Properties: λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5     FITC (Fluorescein) Overview: Excellent fluorescence quantum yield High rate of photobleaching Good solubility in water Broad emission spectrum pH dependent spectra Molecular formula: C20H12O5 Molar mass: 332.3 g/mol Optical Properties: λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC    PE/ATTO 594 PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation Optical Properties: λex = 535 nm λem = 627 nm Laser = 488 to 561 nm    PerCP  Overview: Peridinin-Chlorophyll-Protein Complex Small phycobiliprotein Isolated from red algae Large stokes shift (195 nm) Molecular Weight: 35 kDa   Optical Properties: λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm     R-PE (R-Phycoerythrin) Overview: Broad excitation spectrum High quantum yield Photostable Member of the phycobiliprotein family Isolated from red algae Excellent solubility in water Molecular Weight: 250 kDa   Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC   AP (Alkaline Phosphatase) Properties: Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation Catalyzes the conversion of: Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products Molecular weight: 140 kDa Applications: Western blot, immunohistochemistry, and ELISA HRP (Horseradish peroxidase) Properties: Enzymatic activity is used to amplify weak signals and increase visibility of a target Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors Catalyzes the conversion of: Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL) Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products High turnover rate enables rapid generation of a strong signal 44 kDa glycoprotein Extinction coefficient: 100 (403 nm) Applications: Western blot, immunohistochemistry, and ELISA Biotin Properties: Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity Molar mass: 244.31 g/mol Formula: C10H16N2O3S Applications: Western blot, immunohistochemistry, and ELISA Streptavidin Properties: Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity Molecular weight: 53 kDa Formula: C10H16N2O3S Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein A purified
Clonality	Polyclonal
Specificity	Cross reacts with Aha1.
Cite This Product	Rabbit Anti-Human AHA2 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-196)
Certificate of Analysis	1 µg/ml of SPC-196 was sufficient for detection of Aha2 in 20 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19112384

Alternative Names	ATPase hydrogen-exporting ATPase 2 Antibody
Research Areas	Cancer, Heat Shock
Accession Number	NP_689605.1
Gene ID	130872
Swiss Prot	Q719I0
Scientific Background	The Arabidopsis protein RIN4 is a well known regulator of plant immunity. The plasma membrane H+-ATPases Aha1 and Aha2 are one class of RIN4-associated proteins (1-5). Aha1 and Aha2 play a crucial role in resisting pathogen invasion- plants use RIN4 to regulate H+-ATPase activity during immune responses, thereby controlling stomatal apertures during pathogen attack (1). Wild type AHA2 has been found to be localized to the plasma membrane, and has also been found in the ER (4).
References	1. Liu J., Elmore J.M., and Coaker G. (2009) Plant Signal Behav. 4(12): 1107-1110. 2. Harper J.F., Surowy T.K. and Sussman M.R. (1989) Proc Natl Acad. Sci USA . 86: 1234-1238. 3. Harper J. F., et al. (1990) J Biol Chem. 265: 13601-13608. 4. Harper J.F., Manney L., and Sussman M.R. (1994) Mol Gen Genet. 243: 572-587. 5. Regenberg B., Villalba J.M., Lanfermeijer F.C., and Palmgren M.G. (1995) Plant Cell. 7: 1655-1666.