Anti-AKT3 Antibody

Anti-AKT3 Antibody__Rabbit Anti-Human AKT3 Polyclonal ALW-II-41-27

Product Name	AKT3 Antibody
Description	Rabbit Anti-Human AKT3 Polyclonal
Species Reactivity	Human, Mouse
Applications	, WB , AM
Antibody Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species	Rabbit
Immunogen Species	Human
Immunogen	Synthetic peptide of human Akt3 (AA402-416)
Conjugates	Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated APC (Allophycocyanin) Overview: High quantum yield Large phycobiliprotein 6 chromophores per molecule Isolated from red algae Molecular Weight: 105 kDa APC Datasheet   Optical Properties: λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5     ATTO 390 Overview: High fluorescence yield Large Stokes-shift (89 nm) Good photostability Moderately hydrophilic Good solubility in polar solvents Coumarin derivate, uncharged Low molar mass: 343.42 g/mol  ATTO 390 Datasheet Optical Properties: λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm     ATTO 488 Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation New dye with net charge of -1 Molar Mass: 804 g/mol  ATTO 488 Datasheet    Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC    ATTO 565 Overview: High fluorescence yield High thermal and photostability Good solubility in polar solvents Excellent solubility in water Very little aggregation Rhodamine dye derivative Molar Mass: 611 g/mol ATTO 565 Datasheet   Optical Properties: λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC    ATTO 594 Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation New dye with net charge of -1 Molar Mass: 1137 g/mol ATTO 594 Datasheet   Optical Properties: λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red®    ATTO 633 Overview: High fluorescence yield High thermal and photostability Moderately hydrophilic Good solubility in polar solvents Stable at pH 4 – 11 Cationic dye, perchlorate salt Molar Mass: 652.2 g/mol ATTO 633 Datasheet Optical Properties: λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5    ATTO 655 Overview: High fluorescence yield High thermal and photostability Excellent ozone resistance Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 634 g/mol ATTO 655 Datasheet Optical Properties: λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5    ATTO 680 Overview: High fluorescence yield Excellent thermal and photostability Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 631 g/mol ATTO 680 Datasheet   Optical Properties: λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5    ATTO 700 Overview: High fluorescence yield Excellent thermal and photostability Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 575 g/mol ATTO 700 Datasheet   Optical Properties: λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5     FITC (Fluorescein) Overview: Excellent fluorescence quantum yield High rate of photobleaching Good solubility in water Broad emission spectrum pH dependent spectra Molecular formula: C20H12O5 Molar mass: 332.3 g/mol FITC-Fluorescent-conjugate Optical Properties: λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC    PE/ATTO 594 PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation PE/ATTO 594 Datasheet Optical Properties: λex = 535 nm λem = 627 nm Laser = 488 to 561 nm    PerCP  Overview: Peridinin-Chlorophyll-Protein Complex Small phycobiliprotein Isolated from red algae Large stokes shift (195 nm) Molecular Weight: 35 kDa PerCP Datasheet   Optical Properties: λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm     R-PE (R-Phycoerythrin) Overview: Broad excitation spectrum High quantum yield Photostable Member of the phycobiliprotein family Isolated from red algae Excellent solubility in water Molecular Weight: 250 kDa R-PE Datasheet   Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC   AP (Alkaline Phosphatase) Properties: Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation Catalyzes the conversion of: Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products Molecular weight: 140 kDa Applications: Western blot, immunohistochemistry, and ELISA AP Datasheet HRP (Horseradish peroxidase) Properties: Enzymatic activity is used to amplify weak signals and increase visibility of a target Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors Catalyzes the conversion of: Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL) Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products High turnover rate enables rapid generation of a strong signal 44 kDa glycoprotein Extinction coefficient: 100 (403 nm) Applications: Western blot, immunohistochemistry, and ELISA HRP Datasheet Biotin Properties: Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity Molar mass: 244.31 g/mol Formula: C10H16N2O3S Applications: Western blot, immunohistochemistry, and ELISA Biotin Datasheet Streptavidin Properties: Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity Molecular weight: 53 kDa Formula: C10H16N2O3S Applications: Western blot, immunohistochemistry, and ELISA Streptavidin Datasheet

Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Peptide Affinity Purified
Clonality	Polyclonal
Specificity	Detects 55.775 kDa.
Cite This Product	Rabbit Anti-Human AKT3 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1102)
Certificate of Analysis	A 1:250 dilution of SPC-1102 was sufficient for detection of AKT3 in 10 µg of human A549 cell line lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19112586

Alternative Names	Akt3 antibody, AKT3 kinase antibody, AKT3_HUMAN antibody, PKB gamma antibody, PKBG antibody, PRKBG antibody, Protein kinase Akt-3 antibody, Protein kinase B gamma antibody, RAC gamma antibody, RAC gamma serine/threonine protein kinase antibody, RACPK Gamma antibody, Serine threonine protein kinase Akt 3 antibody, STK 2 antibody, STK-2 antibody, STK2 antibody, V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
Cellular Localization	Cytoplasm, Membrane
Accession Number	NP_005456
Gene ID	10000
Swiss Prot	Q9Y243
Scientific Background	AKT3 is Ser/Thr Kinase that is activated by PIP2 produced by PI3K, leading to translocation of AKT3 to the plasma membrane where it can be activated by S/T phosphorylation by PDK1/2. It is a regulator of cell signalling events such as proliferation, differentiation, apoptosis, tumourigenesis and also plays a role in brain development and in glucose metabolism, in response to insulin and a variety of growth factors such as PDGF and IGF-1. It is upregulated in a number of cancers.