The positive effect of decreasing the oxygen HMPL-013 web concentration in this study
The positive effect of decreasing the oxygen concentration in this study was mostly seen when the embryo was cultured with pyruvate-lactate.ConclusionAn exposure of porcine embryos during the first 2 days of IVC to the increased glucose concentrations increased H2O2 production in Day 1 embryos and impaired the ability of the porcine zygote to cleave and develop to the blastocyst stage. Moreover, results from the this study suggest that the concentration of glucose in the medium that can be used by the day 1? emrbyos is limited to 3.5 mM and exposure to higher glucose concentrations does not improve embryo development. In addition, culturing the embryos under 5 oxygen tension improved embryonic development and embryo quality, especially when applied to early developmental stages cultured in pyruvate-lactate-containing medium.AcknowledgementsThe authors are grateful to Ms. T. Aoki, Ms. E. Ishii, Ms. A. Nakamura and Ms. M. Terui for technical assistance. This study was supported from the Japanese Society for the Promotion of Science (JSPS) by a grant-in-aid for JSPS Postdoctoral Fellowship for Foreign Researchers (P 04588).
Reproductive Biology and EndocrinologyResearchBioMed CentralOpen AccessWomen with endometriosis improved their peripheral antioxidant markers after the application of a high antioxidant dietJennifer Mier-Cabrera1, Tania Aburto-Soto2, Soraya Burrola-M dez2, Luis Jim ez-Zamudio3, Mari C Tolentino4, Esther Casanueva4 and C ar Hern dez-Guerrero*Address: 1Departamento de Biolog Celular, Instituto Nacional de Perinatolog “Isidro Espinosa de los Reyes”, Mexico City, Mexico, 2Departamento de Salud, Universidad Iberoamericana Campus Santa Fe, Mexico City, Mexico, 3Laboratorio de Inmunolog Cl ica I, Departamento de Inmunolog , Escuela Nacional de Ciencias Biol icas, Instituto Polit nico Nacional, Mexico City, Mexico and 4Subdirecci de Investigaci en Salud P lica, Instituto Nacional de Perinatolog PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25768400 “Isidro Espinosa de los Reyes”, Mexico City, Mexico Email: Jennifer Mier-Cabrera – [email protected]; Tania Aburto-Soto – [email protected]; Soraya BurrolaM dez – [email protected]; Luis Jim ez-Zamudio – [email protected]; Mari C Tolentino – [email protected]; Esther Casanueva – [email protected]; C ar Hern dezGuerrero* – [email protected] * Corresponding authorPublished: 28 May 2009 Reproductive Biology and Endocrinology 2009, 7:54 doi:10.1186/1477-7827-7-Received: 21 January 2009 Accepted: 28 MayThis article is available from: http://www.rbej.com/content/7/1/54 ?2009 Mier-Cabrera et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.AbstractBackground: Oxidative stress has been identified in the peritoneal fluid and peripheral blood of women with endometriosis. However, there is little information on the antioxidant intake for this group of women. The objectives of this work were 1) to compare the antioxidant intake among women with and without endometriosis and 2) to design and apply a high antioxidant diet to evaluate its capacity to reduce oxidative stress markers and improve antioxidant markers in the peripheral blood of women with endometriosis. Methods: Women with (WEN, n = 83) and without endometriosis (WWE, n = 80) wer.