Rings from a complete dataset with the southeastern Alps (CRENODAT Project, Biodiversity assessment and integrity evaluation of springs of Trentino–Italian Alps–and long-term ecological study, 2004008 [9]) had been used. We looked for Planothidium angustilanceolatum sp. nov. inside the epibryon samples from ten CRENODAT springs that had been chosen because counts integrated no less than 100 valves of P. lanceolatum s.l. and because they had an ecomorphology/Pregnanediol Data Sheet hydrochemistry consistent with all the sort locality of this species. We also looked for Delicatophycus crassiminutus sp. nov. within the epibryon slides from the 5 LPS integrated inside the CRENODAT Project. All of the statistical analyses have been performed inside the R statistical atmosphere [60]. To find out much more concerning the ecological preferences of Eunotia crassiminor sp. nov. as when compared with Eunotia minor (K z.) Grunow, we thought of the 12 CRENODAT sites and also the 4 EBERs web-sites in which both species happen. If important, by revisiting the slide, we carefully checked the relative abundances of the two species in every single web site. We then calculated weighted typical, mode, percentiles, minimum, and maximum for each and every environmental parameter, tested variations amongst the two species for statistical significance making use of t-tests, and illustrated the preferences from the two species for every factor for which there was a substantial difference with box plots (Figure 3a). The R packages utilised for these Eunotia analyses had been corrplot, weights, and ENmisc. For the biometry a part of the study, all relevant morphologicalDiversity 2021, 13,five ofparameters listed in Table 2 were measured on 100 raphe-valves and one hundred rapheless-valves of Planothidium angustilanceolatum sp. nov. and P. lanceolatum (Br . ex K z.) Lange-Bert., respectively. The R package plotrix was utilised for this Planothidium evaluation.Diversity 2021, 13,three. Outcomes Eunotia crassiminor Lange-Bert. et Cantonati sp. nov. (Figures 1 and two)six Esfenvalerate web ofFigure 1. LM morphology of Eunotia crassiminor Lange-Bert. et Cantonati sp. nov. (a ,k): valve views. (h): girdle Figure 1. LM morphology of Eunotia crassiminor Lange-Bert. et Cantonati sp. nov. (a ,k): valve views. (h): girdle views. (a): initial cell. (j,k): Chromoplast morphology. (i,m): Eunotia minor minor specimens shown for comparison. All views. (a): initial cell. (j,k): Chromoplast morphology. (i,m): Eunotia specimens shown for comparison. All micrographs bright brightwith the exception of 12 which that is primarily based on chlorophyll autofluorescence. bar 10bar 10 . micrographs field, field, with the exception of 12 is primarily based on chlorophyll autofluorescence. Scale Scale .Diversity 2021, 13, 549 Diversity 2021, 13,6 of 20 7 ofFigure 2. (a). SEM photos of Eunotia crassiminor Lange-Bert. et Cantonati sp. nov. (a): External views. (f,g): internal Figure 2. (a). SEM pictures of Eunotia crassiminor Lange-Bert. et Cantonati sp. nov. (a): External views. (f,g): internal views. Scale bars 10 (a ,e,f), 4 (g), three (a ,e,f). views. Scale bars 10 (a ,e,f), four (g), three (a ,e,f).Synonymy. Eunotia minor sensu Lange-Bert. et al. [16], Figure 159: 1. Form material. HOLOTYPE. Diatom collection of your MUSE–Museo delle Scienze, To exclude from synonymy: Eunotia minor sensu Lange-Bert. et al. [16], Figure 159: Trento, Italy, TR, slide cLIM007 DIAT 1971 (Mt. Penna spring, bryophytes). Collected by 127. M. Cantonati on the 25th of July 2011. The holotype material is shown in Figures 1a ,jDifferential diagnosis versus Eunotia minor (K z.) Grunow [referred to Hima.