LH 846

LH 846__Casein kinase 1d inhibitor Integrin Antagonist 2 (hydrochloride)

Product Name LH 846
Description

Casein kinase 1d inhibitor
Purity >98% (HPLC)
CAS No. 639052-78-1
Molecular Formula C16H13ClN2OS
Molecular Weight 316.8
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble to 100 mM in DMSO
Source Synthetic
Appearance White to beige powder
SMILES CC1=CC2=C(C=C1Cl)N=C(S2)NC(=O)CC3=CC=CC=C3
InChI InChI=1S/C16H13ClN2OS/c1-10-7-14-13(9-12(10)17)18-16(21-14)19-15(20)8-11-5-3-2-4-6-11/h2-7,9H,8H2,1H3,(H,18,19,20)
InChIKey DYHAMRNAHTWYKY-UHFFFAOYSA-N
Safety Phrases Classification:
Acute toxicity, Oral (Category 4)
Chronic aquatic toxicity(Category 4)

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.

Hazard statements:
H302 Harmful if swallowed.
H413 May cause long lasting harmful effects to aquatic life.
Cite This Product LH 846 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-503)
Alternative Names LH846, LH-846, N-(5-Chloro-6-methyl-2-benzothiazolyl)benzeneacetamide
Research Areas Cell Signaling
PubChem ID 851474
Scientific Background LH 846 is a potent and selective inhibitor of Casein Kinase CK1δ. Specifically, it inhibits CK1δ-dependent phosphorylation and degradation of PER1 protein. CK1δ is a known regulator of circadian rhythm.
References 1. Lee J.W., et al. (2011) Angewandte Chemie. 50(45): 10608–10611.

Anti-LGI1 Antibody S283-7

Anti-LGI1 Antibody
S283-7__Mouse Anti-Mouse LGI1 Monoclonal IgG2a Taltobulin

Product Name LGI1 Antibody
Description

Mouse Anti-Mouse LGI1 Monoclonal IgG2a
Species Reactivity Human, Mouse, Rat
Applications ,
WB
,
IHC
,
ICC/IF
Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Mouse
Immunogen Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S283-7
Isotype IgG2a
Specificity Detects ~60kDa.
Cite This Product Mouse Anti-Mouse LGI1 Monoclonal, Clone S283-7 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SMC-461)
Certificate of Analysis 1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Alternative Names ADLTE Antibody, ADPAEF Antibody, ADPEAF Antibody, Epitempin 1 Antibody, EPT Antibody, ETL1 Antibody, IB1099 Antibody, leucine rich glioma inactivated 1 Antibody, OTTHUMP00000020121 Antibody, OTTHUMP00000020122
Antibody
Research Areas Cancer, Cell Signaling, Epigenetics, Neurodegeneration, Neuroscience
Cellular Localization Cell Junction, Endoplasmic Reticulum, Golgi apparatus
Accession Number NP_064674.1
Gene ID 56839
Swiss Prot Q9JIA1.1
Scientific Background The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.
References 1. Chernova O.B., Somerville R.P. and Cowell J.K. (1998) Oncogene. 17:2873-81.
2. Fialka F., et al. (2008) Oral Oncol.
3. Gu W., et al. (2005) Mol. Biol. Evol. 22:2209-16.
4. Fukata Y., et al. (2006) Science. 313:1792-5.

LFM A13

LFM A13__BTK kinase inhibitor CFI-400946 (free base)

Product Name LFM A13
Description

BTK kinase inhibitor
Purity >97% (TLC)
CAS No. 62004-35-7
Molecular Formula C11H8Br2N2O2
Molecular Weight 360.0
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble in DMSO (10 mg/ml) or ethanol (0.3 mg/ml)
Source Synthetic
Appearance Yellow solid
SMILES C/C(=C(C#N)/C(=O)NC1=C(C=CC(=C1)Br)Br)/O
InChI InChI=1S/C11H8Br2N2O2/c1-6(16)8(5-14)11(17)15-10-4-7(12)2-3-9(10)13/h2-4,16H,1H3,(H,15,17)
InChIKey UVSVTDVJQAJIFG-UHFFFAOYSA-N
Safety Phrases Classification:
Not a hazardous substance or mixture.

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Cite This Product LFM A13 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-461)
Alternative Names 2-Cyano-N-(2,5-dibromophenyl)-3-hydroxy-2-butenamide
Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors
PubChem ID 54686938
Scientific Background LFM A13 is a potent and selective inhibitor of Bruton's tyrosine kinase (BTK).
References 1. Vassilev A., Ozer Z., Navara C., Mahajan S., & Uckun F.M. (1999). J. Biol. Chem. 274(3): 1646–1656.

Lestaurtinib

Lestaurtinib__FLT3, TrkA kinase inhibitor Grapiprant

Product Name Lestaurtinib
Description

FLT3, TrkA kinase inhibitor
Purity >98% (HPLC)
CAS No. 111358-88-4
Molecular Formula C26H21N3O4
Molecular Weight 439.5
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble to 100 mM in DMSO and to 25 mM in ethanol
Source Synthetic
Appearance White powder
SMILES C@@67(N2C1=C5C(=C4C(=C1C3=C2C=CC=C3)CNC4=O)C8=C(N5email protected(O6)CC@7(O)CO)C=CC=C8)C
InChI InChI=1S/C26H21N3O4/c1-25-26(32,12-30)10-18(33-25)28-16-8-4-2-6-13(16)20-21-15(11-27-24(21)31)19-14-7-3-5-9-17(14)29(25)23(19)22(20)28/h2-9,18,30,32H,10-12H2,1H3,(H,27,31)/t18-,25+,26+/m1/s1
InChIKey UIARLYUEJFELEN-LROUJFHJSA-N
Safety Phrases Classification:
Not a hazardous substance or mixture.

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Cite This Product Lestaurtinib (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-460)
Alternative Names KT5555, CEP-701, (9S,10S,12R)-2,3,9,10,11,12-Hexahydro-10-hydroxy-10-(hydroxymethyl)-9-methyl-9,12-epoxy-1H-diindolo1,2,3-fg:3',2',1'-klpyrrolo3,4-i1,6benzodiazocin-1-one
Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors
PubChem ID 126565
Scientific Background Lestaurtinib is potent JAK2, FLT3 and TrkA receptor tyrosine kinase inhibitor. Specifically, it prevents STAT5 phosphorylation. Lestaurtinib exhibits antiproliferative activity in vitro.
References 1. Weisel K.C., et al. (2007) Ann. NY Acad. Sci. 1106: 190–196.
2. Hexner E.O., et al. (2008) Blood. 111(12): 5663–5671.

Leptomycin B

Leptomycin B__Nuclear export inhibitor Lu AE58055 (Hydrochloride)

Product Name Leptomycin B
Description

Nuclear export inhibitor
Purity >99% (HPLC); NMR (Conforms)
CAS No. 87081-35-4
Molecular Formula C33H48O6
Molecular Weight 540.7
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility May be dissolved into DMSO or ethanol. Use solutions made with DMSO within 24 hours.
Source Synthetic
Appearance Solution in ethanol (0.2 mg/ml)
SMILES CCC(/C=C/email protected1OC(=O)C=CC@@H1C)=C/email protected(C)CC=CC(C)=CC@@H(C)C(=O)C@@H(C)email protected(O)C@@H(C)CC(C)=CC(O)=O
InChI 1S/C33H48O6/c1-9-28(14-15-29-24(5)13-16-31(36)39-29)19-22(3)12-10-11-21(2)17-25(6)32(37)27(8)33(38)26(7)18-23(4)20-30(34)35/h10-11,13-17,19-20,22,24-27,29,33,38H,9,12,18H2,1-8H3,(H,34,35)/b11-10+,15-
InChIKey YACHGFWEQXFSBS-NRQIOVFOSA-N
Safety Phrases Classification:
B2 Flammable liquid – Flammable liquid
D1B Toxic Material Causing Immediate and Serious Toxic Effects – Toxic by ingestion, Toxic by skin absorption

Hazard statement(s):
H225 Highly flammable liquid and vapour.
H301 + H311 Toxic if swallowed or in contact with skin
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H331 Toxic if inhaled.
H370 Causes damage to organs.

Precautionary statement(s):
P210 Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
P260 Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
P280 Wear protective gloves/ protective clothing.
P301 + P310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/ physician.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P307 + P311 IF exposed: Call a POISON CENTER or doctor/ physician.
Cite This Product Leptomycin B (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-534)
Alternative Names Elactocin, LMB, Mantuamycin, NSC 364372
Research Areas Cancer, Cancer Growth Inhibitors
PubChem ID 6917907
Scientific Background Leptomycin B is an inhibitor of nuclear export proteins. It is an anti-fungal, and anti-tumor cytotoxin.

Lenalidomide

Lenalidomide__Ubiquitin ligase inducer Sumanirole (maleate)

Product Name Lenalidomide
Description

Ubiquitin ligase inducer
Purity >98% (TLC); NMR (Conforms)
CAS No. 191732-72-6
Molecular Formula C13H13N3O3
Molecular Weight 259.26
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inducer
Solubility Soluble in DMSO (200 mM), Water (<1 mM) and Ethanol (<1 mM).
Source Synthetic
Appearance Powder
SMILES C1=CC=C(C3=C1C(N(C2C(NC(=O)CC2)=O)C3)=O)N
InChI InChI=1S/C13H13N3O3/c14-9-3-1-2-7-8(9)6-16(13(7)19)10-4-5-11(17)15-12(10)18/h1-3,10H,4-6,14H2,(H,15,17,18)
InChIKey GOTYRUGSSMKFNF-UHFFFAOYSA-N
Safety Phrases Classification: Acute toxicity, Oral (Category 4), Acute toxicity, Dermal (Category 4), Acute toxicity, Inhalation (Category 4), Reproductive toxicity (Category 1B)
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H301- Harmful if swallowed.
H312- Harmful in contact with skin.
H332- Harmful if inhaled.
H360-May damage fertility or the unborn child.
Precautionary statements:
P261- Avoid breathing dust/fumes/gas/mist/vapors/spray.
P263- Avoid contact during pregnancy/while nursing.
P270- Do not eat, drink or smoke when using this product.
P280- Wear protective gloves/protective clothing.
P301+P310- IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P302+P352- IF ON SKIN: Wash with plenty of soap and water.
P304+P340- IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P308+P313- IF exposed or concerned: Get medical advice/attention.
P330- Rinse mouth.
P405- Store locked up.
P501- Dispose of contents/container to a licensed disposal company.
Cite This Product Lenalidomide (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-410)
Alternative Names 3-(7-amino-3-oxo-1H-isoindol-2-yl)piperidine-2,6-dione
Research Areas Cell Signaling, Post-translational Modifications
PubChem ID 216326
Scientific Background Clinically, lenalidomide has proven to be effective in the treatment of multiple myeloma and other B cell diseases. It has the ability to selectively bind and ubiquitinate two lymphoid transcription factors, IKZF1 and IKZF2, via the CRBN-CRL4 ubiquitin ligase. This ultimately leads to the degradation of IKZF1 and IKZF2, which are both essential in myeloma cell survival.
References 1. Krönke J., et al. (2014) Science. 343(6168): 301-5.
2. Lu G., et al. (2014) Science. 343(6168): 305-9.

Leflunomide

Leflunomide__Immunosupressant Z-VAD-FMK

Product Name Leflunomide
Description

Immunosupressant
Purity >98% (TLC: 5:95, CH3OH/CH2Cl2; Rf=0.65); NMR (Conforms)
CAS No. 75706-12-6
Molecular Formula C12H9F3N2O2
Molecular Weight 270.21
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility May be dissolved in DMSO (30 mg/ml); or Ethanol (27 mg/ml)
Source Synthetic
Appearance White crystalline solid
SMILES CC1=C(C=NO1)C(=O)NC2=CC=C(C=C2)C(F)(F)F
InChI InChI=1S/C12H9F3N2O2/c1-7-10(6-16-19-7)11(18)17-9-4-2-8(3-5-9)12(13,14)15/h2-6H,1H3,(H,17,18)
InChIKey VHOGYURTWQBHIL-UHFFFAOYSA-N
Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R25 – Toxic if swallowed
R62 – Possible risk of impaired fertility
R63 – Possible risk of harm to the unborn child
Hazard Phrases:
H301 – Toxic if swallowed.
H315 – Causes skin irritation.
H319 – Causes serious eye irritation
H335 – May cause respiratory irritation
Precautionary Phrases:
P264 – Wash hands thoroughly after handling
P280 – Wear protective gloves/protective clothing/eye protection/ face protection
P301+310 – IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician
P308+313 – If exposed or concerned: Get medical advice/attention
Cite This Product Leflunomide (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-368)
Alternative Names 5-methyl-N-4-(trifluoromethyl) phenyl-isoxazole-4-carboxamide
Research Areas Cell Signaling, Neuroscience
PubChem ID 3899
Scientific Background Leflunomide is an Immuno-suppressant, it inhibits de novo synthesis of pyrimidine nucleotides and the activity of the protein kinase. It is a part of the known “disease-modifying anti-rheumatic drugs” (DMARD). It has been suggested that leflunomide modulates the expansion of activated autoimmune lymphocytes acting on the regression of rheumatoid conditions (1-2). It has been shown to have a double mechanism of action by slowing down and relieving the pain of rheumatoid arthritis. Studies have shown the lethal effects of Leflunomide after administration to mice in various doses (3).
References 1. Fukushima R., Kanamori S., Hirashiba M., et al. (2007) Reprod. Toxical. 24(3-4): 310-316.
2. Smolen JS., Emery P. (2000) Rheumatology. 39: 48-56.
3. Fox R.I., Herrman M.L., Franqou C.G., Wahl G.M., Morns R.E., Strand V. and Kirschbaum B.S. (1999) Clin Immunol 93(3): 198-208.

LDN-57444

LDN-57444__DUB inhibitor LDN-214118

Product Name LDN-57444
Description

DUB inhibitor
Purity >98%
CAS No. 668467-91-2
Molecular Formula C17H11Cl3N2O3
Molecular Weight 397.6
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble in 20 mg/ml DMSO
Source Synthetic
Appearance Yellow Powder
SMILES CC(=O)O/N=C1/C2=C(C=CC(=C2)Cl)N(C1=O)CC3=C(C=CC(=C3)Cl)Cl
InChI InChI=1S/C17H11Cl3N2O3/c1-9(23)25-21-16-13-7-12(19)3-5-15(13)22(17(16)24)8-10-6-11(18)2-4-14(10)20/h2-7H,8H2,1H3/b21-16-
InChIKey OPQRFPHLZZPCCH-PGMHBOJBSA-N
Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
Hazard Statements:
H302 – Harmful if swallowed
H317 – May cause an allergic skin reaction
Precautionary Statements:
P280 – Wear protective gloves.
Cite This Product LDN-57444 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-332)
Alternative Names 3-(O-acetyloxime), 5-chloro-1-(2,5-dichlorophenyl)methyl-1H-Indole-2,3-dione, C30, LDN 57444
Research Areas Cancer, Apoptosis
PubChem ID 16760696
Scientific Background Inhibits ubiquitin C-terminal hydrolase (UCH-L1) (Ki=0.4 M). Decreases proteasome activity and increases levels of ubiquitinated proteins. Induces apoptosis. Causes dramatic alterations in synaptic protein distribution and spine morphology in vivo. Cell permeable.
References 1. Liu Y., et al. (2003) Chem. Biol. 10: 837.
2. Tan Y.Y., et al. (2008) Mol. Cell. Biochem. 318: 109.
3. Cartier A.E., et al. (2009) J.Neurosci. 29: 7857.

Anti-Lck Antibody (pTyr263+pTyr264)

Anti-Lck Antibody (pTyr263+pTyr264)__Rabbit Anti-Human Lck (pTyr263+pTyr264) Polyclonal BAY 80-6947

Product Name Lck Antibody (pTyr263+pTyr264)
Description

Rabbit Anti-Human Lck (pTyr263+pTyr264) Polyclonal
Species Reactivity Human
Applications ,
WB
,
AM
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen A phospho-specific peptide corresponding to residues surrounding Tyr263 and Tyr264 of human Lck (AA257-268)
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet
Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects 57.869 kDa.
Cite This Product Rabbit Anti-Human Lck (pTyr263+pTyr264) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1006)
Certificate of Analysis A 1:250 dilution of SPC-1006 was sufficient for detection of Lck (pTyr263+pTyr264) in 10 µg of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Alternative Names IMD22 Antibody, LCK Antibody, Lck p56 Antibody, LCK proto-oncogene, Src family tyrosine kinase Antibody, Leukocyte C-terminal Src kinase Antibody, LSK Antibody, Lymphocyte cell specific protein tyrosine kinase Antibody, Membrane associated protein tyrosine kinase Antibody, Oncogene lck Antibody, P56 LCK Antibody, p56lck Antibody, pp58 lck Antibody, Protein YT16 Antibody, Proto oncogene tyrosine protein kinase LCK Antibody, Proto-oncogene Lck Antibody, T cell specific protein tyrosine kinase Antibody, T lymphocyte specific protein tyrosine kinase p56lck Antibody, Tyrosine-protein kinase Lck Antibody, YT 16 Antibody
Cellular Localization Cytoplasm, Cell membrane
Accession Number NP_005347
Gene ID 3932
Swiss Prot P06239
Scientific Background LCK is a protein-tyrosine kinase involved in T-cell development and T-cell activation.

Anti-LC3C Antibody VB2

Anti-LC3C Antibody
VB2__Mouse Anti-Human LC3C Monoclonal IgG1 PSI-698

Product Name LC3C Antibody
Description

Mouse Anti-Human LC3C Monoclonal IgG1
Species Reactivity Human, Mouse, Rat
Applications ,
WB
,
IP
Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Peptide derived from C-terminal region of human LC3C protein
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet
Storage Buffer 10 mM sodium phosphate, pH7.2, 150 mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC, 4ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number VB2
Isotype IgG1
Specificity Specific for LC3C. Does not detect LC3A, LC3B, GABARAP, GABARAPL1 or GABARAPL2.
Cite This Product Mouse Anti-Human LC3C Monoclonal, Clone VB2 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SMC-212)
Certificate of Analysis A 1:20000 dilution of SMC-212 was sufficient for detection of LC3C in lysate from cells expressing recombinant GST-tagged LC3C by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Alternative Names MAP1LC3C Antibody, ATG8J Antibody, Autophagy-related protein LC3 C Antibody, Autophagy-related ubiquitin-like modifier LC3 C Antibody, LC3 like protein 2 Antibody, MAP1 light chain 3 like protein 2 Antibody, MAP1 light chain 3 like protein 3 Antibody, MAP1 light chain 3-like protein 3 Antibody, MAP1A/MAP1B LC3 C Antibody, MAP1A/MAP1B light chain 3 C Antibody, MAP1LC3C Antibody, Microtubule-associated protein 1 light chain 3 gamma Antibody, Microtubule-associated proteins 1A/1B light chain 3C Antibody, MLP3C_HUMAN Antibody
Research Areas Cancer, Autophagy
Cellular Localization Cytoplasm, Autophagosome, autophagosome membrane, Cytoplasmic Vesicle, Cytoskeleton
Accession Number NP_001004343.1
Gene ID 440738
Swiss Prot Q9BXW4
Scientific Background Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3C is one of three isoforms which exhibits the lowest expression relative to the other two isoforms. Its highest expression is seen in placenta, lung and ovary.
References 1. Tanida, I., Ueno, T., & Kominami, E. (2008). LC3 and Autophagy. Methods Mol Biol. 445: 77-88.
2. He, H., Dang, Y., Dai, F., Guo, Z., Wu, J., She, X., et al. (2003). J Biol Chem. 278(31): 29278-87.