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Genistein

Genistein__Tyrosine kinase inhibitor ABT-737

Product Name Genistein

Description
Tyrosine kinase inhibitor

Purity >98% (HPLC)

CAS No. 446-72-0

Molecular Formula C15H10O5

Molecular Weight 270.2

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO (30 mg/ml), 100% ethanol (4 mg/ml) or dilute aqueous base; insoluble in water

Source Synthetic

Appearance Creamy off-white solid

SMILES C1=C(O)C=C(C2=C1OC=C(C2=O)C3=CC=C(O)C=C3)O

InChI InChI=1S/C15H10O5/c16-9-3-1-8(2-4-9)11-7-20-13-6-10(17)5-12(18)14(13)15(11)19/h1-7,16-18H

InChIKey TZBJGXHYKVUXJN-UHFFFAOYSA-N

Safety Phrases Classification:
Acute toxicity, Oral (Category 4), H302

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.

Hazard statements:
H302 Harmful if swallowed

Precautionary statements:
P264 Wash skin thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P330 Rinse mouth.
P501 Dispose of contents/ container to an approved waste disposal plant.

Cite This Product Genistein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-446)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125640

Alternative Names 4',5,7-Trihydroxyisoflavone, 5,7-Dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one, 4′,5,7-Trihydroxyisoflavone, 5,7-Dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one

Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors

PubChem ID 5280961

Scientific Background Genistein has a wide range of biological actions. It is an inhibitor of tyrosine protein kinase, including epidermal growth factor receptor kinase. It also inhibits DNA topoisomerase and α-glucosidase. It produces cell cycle arrest and apoptosis, and inhibits endocytosis, as well as, inhibits insulin-induced glucose uptake in adipocytes.

References 1. Akiyama T., et al. (1987) J Biol. Chem. 262(12): 5592–5595.
2. Bazuine M., van den Broek P.J.A., & Maassen J. A. (2005) Biochem. Biophys. Res. Comm. 326(2): 511–514.
3. Linassier C., Pierre M., Le Pecq J.B., & Pierre J. (1990) Biochem. Pharm. 39(1): 187–193.
4. Dean N.M., Kanemitsu M., & Boynton A.L. (1989) Biochem. Biophys. Res. Comm. 165(2): 795–801.

Geldanamycin- FITC

Geldanamycin- FITC__Hsp90 inhibitor AR-C155858

Product Name Geldanamycin- FITC

Description
Hsp90 inhibitor

Purity >98%

CAS No. 30562-34-6

Molecular Formula C55H63N5O13S

Molecular Weight 560.6

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO

Source Synthetic

Appearance Purple Solid

SMILES Cemail protected1CC@@H(C@@H(email protected(/C=C(/C@@H(email protected(/C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)/C)OC)OC(=O)N)C)C)O)OC

InChI InChI=1S/C29H40N2O9/c1-15-11-19-25(34)20(14-21(32)27(19)39-7)31-28(35)16(2)9-8-10-22(37-5)26(40-29(30)36)18(4)13-17(3)24(33)23(12-15)38-6/h8-10,13-15,17,22-24,26,33H,11-12H2,1-7H3,(H2,30,36)(H,31,35)

InChIKey QTQAWLPCGQOSGP-KSRBKZBZSA-N

Safety Phrases Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R68- Possible risk of irreversible effects

Cite This Product Geldanamycin- FITC (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-113)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125636

Alternative Names (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo16.3.1
docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Research Areas Cancer, Heat Shock

PubChem ID 5288382

Scientific Background Geldanamycin is a benzoquinoid ansamycin produced by Streptomyces hygroscopicus. It binds specifically to heat shock protein HSP90 and downregulates target proteins including tyrosine kinases, steroid receptors, transcription factors and cell cycle regulatory kinases (1,2). It induces the inactivation, destabilization and eventual degradation of HIF-1α (3). It is also an inhibitor of pp60src tyrosine kinase and of c-myc gene expression in murine lymphoblastoma cells. It inhibits the transforming activity of abl, erbB, fps, src, and yes (4). Geldanamycin is capable of destabilizing several oncogene and proto-oncogene products; it is a potent inhibitor of the nuclear hormone receptor family (5). It protects against α-synuclein toxicity to dopaminergic neurons in Drosophila, and destabilizes mutant p53 protein from a number of breast, leukemic, and prostate cell lines (6). Inhibits basal and hypoxia-induced expression of c-Jun (IC50=75nM) and abolishes hypoxia-induced increase in c-Jun N-terminal kinase (JNK) activity. Inhibits telomerase activity through inhibition of HSP90, a chaperone required for the assembly and activation of telomerase in human cells (6). It is ~10-fold more potent than herbimycin A. Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References 1. Whitesell L., et al. (1994) Proc. Natl. Acad. Sci. USA 91:8324.
2. Neckers L. (2002) Trends Mol. Med. 8: S55.
3. Mabjeesh N.J., et al. (2002) Cancer Res. 62: 2478.
4. Chavany C., et al. 1996) Amer. Society Biochem Mol Bio. 9: 4974-4977.
5. Villa R., et al. (2003) Carcinogenesis. 24(5): 851-9.
6. Yamaki H., Iguchi-Ariga S.M., and Ariga H. (1989) J Antibiot (Tokyo). 42(4): 604-10.

Geldanamycin- Biotin

Geldanamycin- Biotin__Hsp90 inhibitor WAY-362450

Product Name Geldanamycin- Biotin

Description
Hsp90 inhibitor

Purity >98%

CAS No. 30562-34-6

Molecular Formula C55H87N7O17S

Molecular Weight 560.6

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO

Source Synthetic

Appearance Purple Solid

SMILES Cemail protected1CC@@H(C@@H(email protected(/C=C(/C@@H(email protected(/C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)/C)OC)OC(=O)N)C)C)O)OC

InChI InChI=1S/C29H40N2O9/c1-15-11-19-25(34)20(14-21(32)27(19)39-7)31-28(35)16(2)9-8-10-22(37-5)26(40-29(30)36)18(4)13-17(3)24(33)23(12-15)38-6/h8-10,13-15,17,22-24,26,33H,11-12H2,1-7H3,(H2,30,36)(H,31,35)

InChIKey QTQAWLPCGQOSGP-KSRBKZBZSA-N

Safety Phrases Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R68- Possible risk of irreversible effects

Cite This Product Geldanamycin- Biotin (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-112)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125518

Alternative Names (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo16.3.1
docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Research Areas Cancer, Heat Shock

PubChem ID 5288382

Scientific Background Geldanamycin is a benzoquinoid ansamycin produced by Streptomyces hygroscopicus. It binds specifically to heat shock protein HSP90 and downregulates target proteins including tyrosine kinases, steroid receptors, transcription factors and cell cycle regulatory kinases (1,2). It induces the inactivation, destabilization and eventual degradation of HIF-1α (3). It is also an inhibitor of pp60src tyrosine kinase and of c-myc gene expression in murine lymphoblastoma cells. It inhibits the transforming activity of abl, erbB, fps, src, and yes (4). Geldanamycin is capable of destabilizing several oncogene and proto-oncogene products; it is a potent inhibitor of the nuclear hormone receptor family (5). It protects against α-synuclein toxicity to dopaminergic neurons in Drosophila, and destabilizes mutant p53 protein from a number of breast, leukemic, and prostate cell lines (6). Inhibits basal and hypoxia-induced expression of c-Jun (IC50=75nM) and abolishes hypoxia-induced increase in c-Jun N-terminal kinase (JNK) activity. Inhibits telomerase activity through inhibition of HSP90, a chaperone required for the assembly and activation of telomerase in human cells (6). It is ~10-fold more potent than herbimycin A. Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References 1. Whitesell L., et al. (1994) Proc. Natl. Acad. Sci. USA 91:8324.
2. Neckers L. (2002) Trends Mol. Med. 8: S55.
3. Mabjeesh N.J., et al. (2002) Cancer Res. 62: 2478.
4. Chavany C., et al. 1996) Amer. Society Biochem Mol Bio. 9: 4974-4977.
5. Villa R., et al. (2003) Carcinogenesis. 24(5): 851-9.
6. Yamaki H., Iguchi-Ariga S.M., and Ariga H. (1989) J Antibiot (Tokyo). 42(4): 604-10.

Geldanamycin

Geldanamycin__Hsp90 inhibitor Exherin (trifluoroacetate)

Product Name Geldanamycin

Description
Hsp90 inhibitor

Purity >95%

CAS No. 30562-34-6

Molecular Formula C29H40N2O9

Molecular Weight 560.6

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Slightly soluble in methanol, chloroform or DMSO (10 mg/ml); insoluble in water

Source Produced by fermentation

Appearance Yellow Solid

SMILES Cemail protected1CC@@H(C@@H(email protected(/C=C(/C@@H(email protected(/C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)/C)OC)OC(=O)N)C)C)O)OC

InChI InChI=1S/C29H40N2O9/c1-15-11-19-25(34)20(14-21(32)27(19)39-7)31-28(35)16(2)9-8-10-22(37-5)26(40-29(30)36)18(4)13-17(3)24(33)23(12-15)38-6/h8-10,13-15,17,22-24,26,33H,11-12H2,1-7H3,(H2,30,36)(H,31,35)

InChIKey QTQAWLPCGQOSGP-KSRBKZBZSA-N

Safety Phrases Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R68- Possible risk of irreversible effects

Cite This Product Geldanamycin (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-111)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125299

Alternative Names (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo16.3.1
docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Research Areas Cancer, Heat Shock

PubChem ID 5288382

Scientific Background Glendanamycin (GA), a benzoquinone ansamycin antibiotic, interferes with the action of Hsp90 leading to degradation of Hsp90 client proteins. GA itself however has undesirable properties such as poor aqueous solubility and liver toxicity; therefore, numerous analogs have been synthesized, such as 17-AAG(1). 17-AAG is an HSP-90 inhibitor that displays a 100-fold higher affinity for HSP-90 derived from tumor cells compared to HSP-90 from normal cells(2). 17-AAG inhibits Akt activation and expression in tumors and synergizes with a number of antitumor agents such as taxol(3), cisplatin(4) and UCN-01 (400 nM 17-AAG, U937 cells)(5). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References 1. Whitesell L., et al. (1994) Proc. Natl. Acad. Sci. USA 91:8324.
2. Neckers L. (2002) Trends Mol. Med. 8: S55.
3. Mabjeesh N.J., et al. (2002) Cancer Res. 62: 2478.
4. Chavany C., et al. 1996) Amer. Society Biochem Mol Bio. 9: 4974-4977.
5. Villa R., et al. (2003) Carcinogenesis. 24(5): 851-9.
6. Yamaki H., Iguchi-Ariga S.M., and Ariga H. (1989) J Antibiot (Tokyo). 42(4): 604-10.

Gefitinib

Gefitinib__EGFR Kinase inhibitor Savolitinib

Product Name Gefitinib

Description
EGFR Kinase inhibitor

Purity >99%

CAS No. 184475-35-2

Molecular Formula C22H24ClFN4O3

Molecular Weight 446.9

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO (89 mg/ml at 25 °C), methanol (20 mg/ml), ethanol (4 mg/ml at 25 °C), DMF (20 mg/ml), and water (<1 mg/ml at 25 °C)

Source Synthetic

Appearance Solid

SMILES C1=C3C(=CC(=C1OC)OCCCN2CCOCC2)C(=NC=N3)NC4=CC=C(C(=C4)Cl)F

InChI InChI=1S/C22H24ClFN4O3/c1-29-20-13-19-16(12-21(20)31-8-2-5-28-6-9-30-10-7-28)22(26-14-25-19)27-15-3-4-18(24)17(23)11-15/h3-4,11-14H,2,5-10H2,1H3,(H,25,26,27)

InChIKey XGALLCVXEZPNRQ-UHFFFAOYSA-N

Safety Phrases Classification:
Not a hazardous substance or mixture.

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.

Cite This Product Gefitinib (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-445)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125249

Alternative Names N-(3-chloro-4-fluorophenyl)-7-methoxy-6-(3-morpholin-4-ylpropoxy)quinazolin-4-amine

Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors

PubChem ID 123631

Scientific Background Gefitinib is a selective inhibitor of EGFR tyrosine kinase by blocking EGFR autophosphorylation.

References 1. Ciardiello F., et al. (2000) Clin. Cancer Res. 6(5): 2053–2063.

Anti-GDNF Antibody

Anti-GDNF Antibody__Rabbit Anti-Human GDNF Polyclonal Sobetirome

Product Name GDNF Antibody

Description
Rabbit Anti-Human GDNF Polyclonal

Species Reactivity Human, Rat

Applications ,
WB
,
ICC/IF

Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen Synthetic peptide from the mid-protein of Human GDNF

Concentration 1 mg/ml

Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)

Overview:

High quantum yield

Large phycobiliprotein

6 chromophores per molecule

Isolated from red algae

Molecular Weight: 105 kDa

APC Datasheet
Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

ATTO 390

Overview:

High fluorescence yield

Large Stokes-shift (89 nm)

Good photostability

Moderately hydrophilic

Good solubility in polar solvents

Coumarin derivate, uncharged

Low molar mass: 343.42 g/mol

ATTO 390 Datasheet
Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

ATTO 488

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 804 g/mol

ATTO 488 Datasheet
Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 565

Overview:

High fluorescence yield

High thermal and photostability

Good solubility in polar solvents

Excellent solubility in water

Very little aggregation

Rhodamine dye derivative

Molar Mass: 611 g/mol

ATTO 565 Datasheet
Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 594

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 1137 g/mol

ATTO 594 Datasheet
Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 633

Overview:

High fluorescence yield

High thermal and photostability

Moderately hydrophilic

Good solubility in polar solvents

Stable at pH 4 – 11

Cationic dye, perchlorate salt

Molar Mass: 652.2 g/mol

ATTO 633 Datasheet
Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 655

Overview:

High fluorescence yield

High thermal and photostability

Excellent ozone resistance

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 634 g/mol

ATTO 655 Datasheet
Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 680

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 631 g/mol

ATTO 680 Datasheet
Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 700

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 575 g/mol

ATTO 700 Datasheet
Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield

High rate of photobleaching

Good solubility in water

Broad emission spectrum

pH dependent spectra

Molecular formula: C₂₀H₁₂O₅

Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate
Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

PE/ATTO 594 Datasheet
Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex

Small phycobiliprotein

Isolated from red algae

Large stokes shift (195 nm)

Molecular Weight: 35 kDa

PerCP Datasheet
Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum

High quantum yield

Photostable

Member of the phycobiliprotein family

Isolated from red algae

Excellent solubility in water

Molecular Weight: 250 kDa

R-PE Datasheet
Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols

Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation

Catalyzes the conversion of:

Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products

Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products

Molecular weight: 140 kDa

Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target

Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors

Catalyzes the conversion of:

Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products

Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)

Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products

High turnover rate enables rapid generation of a strong signal

44 kDa glycoprotein

Extinction coefficient: 100 (403 nm)

Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity

Molar mass: 244.31 g/mol

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity

Molecular weight: 53 kDa

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Storage Buffer PBS, 50% glycerol, 0.09% sodium azide

Storage Temperature -20ºC

Shipping Temperature Blue Ice or 4ºC

Purification Peptide Affinity Purified

Clonality Polyclonal

Specificity Detects ~24 kDa.

Cite This Product Rabbit Anti-Human GDNF Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-710)

Certificate of Analysis A 1:1000 dilution of SPC-710 was sufficient for detection of GDNF in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125196

Alternative Names Glial cell line-derived neurotrophic factor Antibody, Astrocyte-derived trophic factor Antibody, Astrocyte derived trophic factor 1 Antibody, Glial cell derived neurotrophic factor Antibody, ATF 1 Antibody, ATF 2 Antibody, Glial Cell Line Derived Neurotrophic Factor Antibody, ATF Antibody, Atf Antibody, HSCR3 Antibody, Astrocyte derived trophic factor Antibody, ATF2 Antibody, GDNF_HUMAN Antibody, GDNF Antibody, Glial derived neurotrophic factor Antibody, ATF1 Antibody, gdnf Antibody, HFB1 GDNF Antibody, hGDNF Antibody

Research Areas ALS Disease, Neurodegeneration, Neuroscience, Parkinson_s Disease

Cellular Localization Secreted

Accession Number NP_000505.1

Gene ID 2668

Swiss Prot P39905

GDC 0941

GDC 0941__pan-PI3K kinase inhibitor Elamipretide

Product Name GDC 0941

Description
pan-PI3K kinase inhibitor

Purity >98%

CAS No. 957054-30-7

Molecular Formula C23H27N7O3S2

Molecular Weight 513.63

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO

Source Synthetic

Appearance White solid

SMILES CS(=O)(=O)N1CCN(CC1)CC2=CC3=C(S2)C(=NC(=N3)C4=C5C=NNC5=CC=C4)N6CCOCC6

InChI InChI=1S/C23H27N7O3S2/c1-35(31,32)30-7-5-28(6-8-30)15-16-13-20-21(34-16)23(29-9-11-33-12-10-29)26-22(25-20)17-3-2-4-19-18(17)14-24-27-19/h2-4,13-14H,5-12,15H2,1H3,(H,24,27)

InChIKey LHNIIDJUOCFXAP-UHFFFAOYSA-N

Safety Phrases Classification:
Not a hazardous substance or mixture.

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.

Cite This Product GDC 0941 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-501)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125169

Alternative Names Pictrelisib, Pictilisib, GNE 0941, 2-(1H-indazol-4-yl)-6-4-(methylsulfonyl)-1-piperazinylmethyl-4-(4-morpholinyl)-thieno3,2-dpyrimidine

Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors

PubChem ID 17755052

Scientific Background GDC 0941 is a potent inhibitor of the PI 3-kinase family. Specifically, GDC 0941 has been shown to inhibit PI3Kα, PI3Kβ, PI3Kδ and PI3Kγ.

References 1. Zheng J., Wang H., Yao J., & Zou X. (2014) Die Pharmazie. 69(1): 38–42.

Garcinol

Garcinol__HAT inhibitor AICAR

Product Name Garcinol

Description
HAT inhibitor

Purity >98% (TLC); NMR (Conforms)

CAS No. 78824-30-3

Molecular Formula C38H50O6

Molecular Weight 602.8

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in 25 mg/ml DMSO or 25 mg/ml Ethanol

Source Synthetic

Appearance Yellow solid

SMILES CC(=CCC@@H1CC@@2(C(=C(C(=O)C@@(C2=O)(C1(C)C)CC=C(C)C)C(=O)C3=CC(=C(C=C3)O)O)O)Cemail protected(CC=C(C)C)C(=C)C)C

InChI InChI=1S/C38H50O6/c1-22(2)11-13-27(25(7)8)20-37-21-28(15-12-23(3)4)36(9,10)38(35(37)44,18-17-24(5)6)34(43)31(33(37)42)32(41)26-14-16-29(39)30(40)19-26/h11-12,14,16-17,19,27-28,39-41H,7,13,15,18,20-21

InChIKey DTTONLKLWRTCAB-UDFURZHRSA-N

Safety Phrases Classification: Caution- Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes

Cite This Product Garcinol (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-355)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125160

Alternative Names Camboginol, 3-(3,4-dihydroxybenzoyl)-4-hydroxy-8,8-dimethyl-1,7-bis(3-methyl-2-buten-1-yl)-5-(2S)-5-methyl-2-(1-methylethenyl)-4-hexen-1-yl-bicyclo3.3.1non-3-ene-2,9-dione

Research Areas Cell Signaling, Epigenetics

PubChem ID 5490884

Scientific Background Potent inhibitor of histone acetyl transferases p300 (IC50=7 mM) and pCAF (IC50=5 mM) (1). Induces apoptosis in various cell lines (2). Promotes expansion of human hematopoietic stem cells(3). Cell permeable.

References 1. Balasubramanyam K., et al., (2004) J. Biol. Chem. 279: 33716.
2. Parasramka M.A., et al. (2011) Nutr. Cancer. 63: 456.
3. Nishino T., et al. (2011) Nutr. PLoS One 6: e24298.

Anti-GAPDH Antibody

Anti-GAPDH Antibody__Rabbit Anti-Human GAPDH Polyclonal ACY-738

Product Name GAPDH Antibody

Description
Rabbit Anti-Human GAPDH Polyclonal

Species Reactivity Human

Applications ,
WB
,
ICC/IF

Antibody Dilution WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen Synthetic peptide from the mid-protein of Human GAPDH

Concentration 1 mg/ml

Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)

Overview:

High quantum yield

Large phycobiliprotein

6 chromophores per molecule

Isolated from red algae

Molecular Weight: 105 kDa

APC Datasheet
Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

ATTO 390

Overview:

High fluorescence yield

Large Stokes-shift (89 nm)

Good photostability

Moderately hydrophilic

Good solubility in polar solvents

Coumarin derivate, uncharged

Low molar mass: 343.42 g/mol

ATTO 390 Datasheet
Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

ATTO 488

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 804 g/mol

ATTO 488 Datasheet
Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 565

Overview:

High fluorescence yield

High thermal and photostability

Good solubility in polar solvents

Excellent solubility in water

Very little aggregation

Rhodamine dye derivative

Molar Mass: 611 g/mol

ATTO 565 Datasheet
Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 594

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 1137 g/mol

ATTO 594 Datasheet
Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 633

Overview:

High fluorescence yield

High thermal and photostability

Moderately hydrophilic

Good solubility in polar solvents

Stable at pH 4 – 11

Cationic dye, perchlorate salt

Molar Mass: 652.2 g/mol

ATTO 633 Datasheet
Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 655

Overview:

High fluorescence yield

High thermal and photostability

Excellent ozone resistance

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 634 g/mol

ATTO 655 Datasheet
Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 680

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 631 g/mol

ATTO 680 Datasheet
Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 700

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 575 g/mol

ATTO 700 Datasheet
Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield

High rate of photobleaching

Good solubility in water

Broad emission spectrum

pH dependent spectra

Molecular formula: C₂₀H₁₂O₅

Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate
Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

PE/ATTO 594 Datasheet
Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex

Small phycobiliprotein

Isolated from red algae

Large stokes shift (195 nm)

Molecular Weight: 35 kDa

PerCP Datasheet
Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum

High quantum yield

Photostable

Member of the phycobiliprotein family

Isolated from red algae

Excellent solubility in water

Molecular Weight: 250 kDa

R-PE Datasheet
Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols

Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation

Catalyzes the conversion of:

Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products

Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products

Molecular weight: 140 kDa

Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target

Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors

Catalyzes the conversion of:

Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products

Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)

Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products

High turnover rate enables rapid generation of a strong signal

44 kDa glycoprotein

Extinction coefficient: 100 (403 nm)

Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity

Molar mass: 244.31 g/mol

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity

Molecular weight: 53 kDa

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Storage Buffer PBS, 50% glycerol, 0.09% sodium azide

Storage Temperature -20ºC

Shipping Temperature Blue Ice or 4ºC

Purification Peptide Affinity Purified

Clonality Polyclonal

Specificity Detects ~36 kDa.

Cite This Product Rabbit Anti-Human GAPDH Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-689)

Certificate of Analysis A 1:1000 dilution of SPC-689 was sufficient for detection of GAPDH in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125127

Alternative Names CDABP0047 Antibody, GAPD Antibody, GAPDH Antibody, OK/SW-cl.12 Antibody, Peptidyl-cysteine S-nitrosylase GAPDH Antibody, Glyceraldehyde-3-phosphate dehydrogenase Antibody

Research Areas Cancer, Alzheimer_s Disease, Autophagy, Carbohydrate Metabolism, Cell Signaling, Energy Transfer Pathways, Metabolism, Neurodegeneration, Neuroscience

Cellular Localization Cytoplasm, Cytoskeleton, Cytosol, Membrane, Nucleus, Perinuclear Region

Accession Number NP_001243728.1

Gene ID 2597

Swiss Prot P04406

Galanthamine HBr

Galanthamine HBr__Acetylcholinesterase inhibitor GS967

Product Name Galanthamine HBr

Description
Acetylcholinesterase inhibitor

Purity >97% (HPLC); NMR (Conforms)

CAS No. 04-04-53

Molecular Formula C17H22BrNO3

Molecular Weight 368.3

Storage Temperature -20ºC

Shipping Temperature Blue Ice or 4ºC

Product Type Inhibitor

Solubility Soluble in water (12 mg/ml) or in DMSO (10 mg/ml)

Source Synthetic

Appearance White powder

SMILES Br-.COc1ccc2CN(C)CCC@@34C=Cemail protected(O)CC@@H3Oc1c24.H+

InChI 1S/C17H21NO3.BrH/c1-18-8-7-17-6-5-12(19)9-14(17)21-16-13(20-2)4-3-11(10-18)15(16)17;/h3-6,12,14,19H,7-10H2,1-2H3;1H/t12-,14-,17-;/m0./s1

InChIKey QORVDGQLPPAFRS-XPSHAMGMSA-N

Safety Phrases Classification:
D1B Toxic Material Causing Immediate and Serious Toxic Effects – Toxic by ingestion

Hazard statement(s):
H301 Toxic if swallowed.

Precautionary statement(s):
P301 + P310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/ physician.

Cite This Product Galanthamine HBr (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-530)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19125065

Alternative Names Galantamine hydrobromide, Galantamine HBr, Galanthamine hydrobromide, Galanthamine, Galanthamine hydrogen bromide, SPECTRUM1501202, 1,2,3,4,6,7,7a,11c-Octahydro-9-methoxy-2-methyl-benzofuro(4,3,2-efg)(2)benzazocin-6-ol HBr, LS-185021, Galanthamine, Nivalin, Reminyl, Jilkon hydrobromide, Lycoremine hydrobromide

Research Areas Cancer, Alzheimer_s Disease, Apoptosis, Neurodegeneration, Neuroscience

PubChem ID 121587

Scientific Background Galantamine hydrobromide is a long-acting, active acetylcholinesterase inhibitor (IC50 = 410 nM) and allosteric potentiator at neuronal nicotinic ACh receptors. It prevents β-amyloid-induced apoptosis in SH-SY5Y. Long-term administration in a mouse model of Alzheimer's disease reduces amyloid precursor protein deposition and neurodegeneration in these mice.