Ferrostatin-1

Ferrostatin-1__Ferroptosis Inhibitor SAR405838

Product Name Ferrostatin-1
Description

Ferroptosis Inhibitor
Purity >98% (TLC); NMR (Conforms)
CAS No. 347174-05-4
Molecular Formula C15H22N2O2
Molecular Weight 262.4
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble in DMSO (100 mg/ml) or ethanol (100 mg/ml)
Source Synthetic
Appearance Tan powder
SMILES CCOC(=O)c1ccc(c(c1)N)NC2CCCCC2
Safety Phrases Classification:
Skin irritation (Category 2), H315

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.

Hazard statements:
H315 Causes skin irritation.
H319 Causes serious eye irritation.
Precautionary statements:
P260 Do not breathe dust/fume/gas/mist/vapours/spray P264 Wash skin thoroughly after handling.
P280 Wear protective gloves.
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + 340 If INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
P305 + 351+ + 338 IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do so- continue rinsing.
P333 + 313 If skin irritation or a rash occurs: Get medical advice/attention
Cite This Product Ferrostatin-1 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-525)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124089,12742553,12374125,11373172,10804275,10735553,10068507,9746749,9723863,9584832,9551662,8985669,8410813,8182577,7629694,7561720,7069664,7013465,7013462,6816927,6780311,6774387,6766424,6681867,6615970,6418612,6374134,6138185,3968657,3783416,3644887,3221154,3216859,2887340,2868829,2809481,2621713,2476350,2373977,1968808,1936924,1795219,1679702,1552486,1404108,1390618,630473,571734,529129

Alternative Names Ethyl 3-amino-4-(cyclohexylamino)benzoate
Research Areas Cancer, Apoptosis, Cell Signaling, Neuroscience
Scientific Background Ferrostatin-1 inhibits ferroptosis (EC50=60 nM), an iron-dependent form of nonapoptotic cell death. It is especially potent of ferroptosis in cancer cells and glutamate-induced cell death in organotypic rat brain slices (1). It also blocks the cytotoxic effects of sorafenib in hepatocellular carcinoma cells (2), and inhibits oxidative lipid damage and cell death in diverse disease models (3).
References 1. Dixon S.J., et al. (2012) Cell. 149: 1060.
2. Louandre C., et al. (2013) Int. J. Cancer. 133: 1732.
3. Skouta R., et al. (2014) J. Am. Chem. Soc. 136: 4551.

Felodipine

Felodipine__L-type Ca channel blocker Celastrol

Product Name Felodipine
Description

L-type Ca channel blocker
Purity >98% (TLC); NMR (Conforms)
CAS No. 72509-76-3
Molecular Formula C18H19Cl2NO4
Molecular Weight 384.25
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble to 100 mM in ethanol and to 100 mM in DMSO
Source Synthetic
Appearance Light Yellow Solid
SMILES CCOC(=O)C1=C(NC(=C(C1C2=C(C(=CC=C2)Cl)Cl)C(=O)OC)C)C
InChI InChI=1S/C18H19Cl2NO4/c1-5-25-18(23)14-10(3)21-9(2)13(17(22)24-4)15(14)11-7-6-8-12(19)16(11)20/h6-8,15,21H,5H2,1-4H3
InChIKey RZTAMFZIAATZDJ-UHFFFAOYSA-N
Safety Phrases Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with shin and eyes
Risk Phrases:
R20/21/22 – Harmful by inhilation, in contact with skin and if swallowed
R62 – Possible risk of impaired fertility
Hazard Phrases:
H302
Cite This Product Felodipine (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-323)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124046

Alternative Names Ethyl methyl 4-(2,3-dichlorophenyl)-2,6-dimethyl-1,4-dihydro-3,5-pyridinedicarboxylate, Plendil
Research Areas Calcium Channels, Ion Channels, Neuroscience, Voltage-Gated Calcium Channels
PubChem ID 3333
Scientific Background Felodipine is an L-type Ca2+ channel blocker (1). In isolated rat ventricular myocytes, using a whole-cell patch-clamp technique, it displays a Ki of 11 nM (2). It is a clinically useful antihypertensive agent (3) which attenuates the hypertrophic effect of cyclosporine on transplanted hearts (4).
References 1. Furakawa T., et al. (2005) J Cardiovasc. Pharmacol. 45(3): 241-246.
2. Zahradnikova Z., Minarovic I., Zahradnik I. (2007) J Pharmacol Exp. Ther. 322(2): 638-645.
3. Manzo B.A., Matalka M.S., Ravnan S.L. (2003) Pharmacotherapy. 23(11): 1508-1512.
4. Schwitter J., et al. (1999) J Heart Lung Transplant. 18(10): 1003-1013.

FCR1

FCR1__FRET ratiometric fluorescence-based redox sensor GSK1324726A

Product Name FCR1
Description

FRET ratiometric fluorescence-based redox sensor
Molecular Formula C34H37N7O6
Molecular Weight 639
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Product Type Redox Probe
Solubility Soluble in DMSO
Source Synthetic
Appearance Orange Solid
Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Cite This Product FCR1 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-180)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124024/

Alternative Names 7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzogpteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide (FCR1)
Research Areas Cancer, Oxidative Stress
Scientific Background FCR1 or flavin coumarin redox sensor 1, is a novel ratiometric fluorescent redox sensor utilized in fluorescence lifetime imaging microscopy and flow cytometry. In the oxidized form, excitation of FCR1 at 405nm results in a green fluorescence with max emissions at 525nm. Treatment with a mild reducing agent (including sodium cyanoborohydride, DTT, and glutathione) reduces the flavin thereby decreasing the green fluorescence intensity, and increasing the blue. Re-oxidation can be achieved using mild oxidizing agents. In general, the FCR1 probe can be used to observe changes in oxidative capacity without interference from background effects.
References 1. Kaur A., Haghighatbin M.A., Hogan C.F., and New E.J. (2015) Chem. Commun. Epub.

FCCP

FCCP__MPTP inhibitor GANT 61

Product Name FCCP
Description

MPTP inhibitor
Purity >98%
CAS No. 370-86-5
Molecular Formula C10H5F3N4O
Molecular Weight 254.17
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inducer
Solubility Soluble to 100 mM in DMSO
Source Synthetic
Appearance Yellow solid
SMILES C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F
InChI InChI=1S/C2H6FP/c3-1-2-4/h1-2,4H2
InChIKey GYQDBNYYNSTQTM-UHFFFAOYSA-N
Safety Phrases Classification: Toxic. May be harmful or fatal if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R23/24/25- Toxic by inhalation, in contact with skin, and if swallowed
Hazard Phrases:
H301-H317-H319-H413
Precautionary Phrases:
P280-P301 + P310-P305 + P351 + P338
Cite This Product FCCP (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-222)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124024

Alternative Names {4-(Trifluoromethoxy)phenylhydrazono}malononitrile
Research Areas Cancer, Apoptosis
PubChem ID 3330
Scientific Background FCCP is a tool for inhibiting mitochondrial oxidative phosphorylation (1, 2).
References 1. Collins T.J., et al. (2000) Biochem J. 347: 593.
2. Keij J.F., et al. (2000) Cytometry 39: 203.

Fasudil

Fasudil__ROCK inhibitor STING agonist-1

Product Name Fasudil
Description

ROCK inhibitor
Purity >98% (TLC); NMR (Conforms)
CAS No. 103745-39-7
Molecular Formula C14H17N3O2S•HCl
Molecular Weight 327.83
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility May be dissolved in DMSO (75 mg/ml) or water (100 mg/ml)
Source Synthetic
Appearance Powder
SMILES C1CNCCN(C1)S(=O)(=O)C2=CC=CC3=C2C=CN=C3
InChI InChI=1S/C14H17N3O2S/c18-20(19,17-9-2-6-15-8-10-17)14-4-1-3-12-11-16-7-5-13(12)14/h1,3-5,7,11,15H,2,6,8-10H2
InChIKey NGOGFTYYXHNFQH-UHFFFAOYSA-N
Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Cite This Product Fasudil (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-371)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124014

Alternative Names 1-(5-Isoquinolinylsulfonyl) homopiperazine hydrochloride; 5-(1,4-Diazepan-1-ylsulfonyl)isoquinoline
Research Areas Cell Signaling, Neuroscience
PubChem ID 3547
Scientific Background Fasudil is a ROCK2 inhibitor with some action on PRK2 and MSK1 (IC50 values are 1.9 µM, 4 µM and 5 µM respectively). It is also shown to inhibit RSK1, RSK2, PKA, AMPK and PKd1. It is also a calcium channel antagonist and vasodilator.
References 1. Shirotani M., et al. (1991) J. Pharmacol. Exp. Ther. 259(2): 738-744.
2. Arai M., Nozawa R., et al. (1993) Biochem. Pharmacol. 46(8): 1487-1490.
3. Satoh S., et al. (1996) Br. J. Pharmacol. 118(7): 1592-1606.
4. Sward K., et al. (2000) J. Physiol. 522: 33-49.

Anti-FAK Antibody (pTyr577)

Anti-FAK Antibody (pTyr577)__Rabbit Anti-Human FAK (pTyr577) Polyclonal Iopamidol

Product Name FAK Antibody (pTyr577)
Description

Rabbit Anti-Human FAK (pTyr577) Polyclonal
Species Reactivity Human
Applications ,
WB
,
AM
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen A phospho-specific peptide corresponding to residues surrounding Tyr577 of human FAK (AA574-580)
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet
Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects 119.233 kDa.
Cite This Product Rabbit Anti-Human FAK (pTyr577) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-978)
Certificate of Analysis A 1:250 dilution of SPC-978 was sufficient for detection of FAK (pTyr577) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124005

Alternative Names FADK1 Antibody, FADK Antibody, FAK related non kinase polypeptide Antibody, FAK1 Antibody, FAK Antibody, Focal adhesion kinase 1 Antibody, Focal adhesion kinase isoform FAK Del33 Antibody, Focal adhesion kinase related nonkinase Antibody, FRNK Antibody, p125FAK Antibody, PPP1R71 Antibody, Protein phosphatase 1 regulatory subunit 71 Antibody, Protein tyrosine kinase 2 Antibody, Ptk2 Antibody, PTK2 protein tyrosine kinase 2 Antibody
Cellular Localization Cell Junctions, Cell membrane, Focal Adhesion
Accession Number NP_722560
Gene ID 5747
Swiss Prot Q05397
Scientific Background FAK, also known as PTK2, is a non-receptor protein-tyrosine kinase that is activated by tyrosine-phosphorylation in response to integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands. It serves as adaptor protein. It is linked to the MAPK pathway via Grb2, and is also linked to PI3K and PLC gamma pathways. It plays a role as a scaffold for MDM2-mediated p53 degradation, and has been implicated in regulation of cell-cell contacts, microtubule stability and control of gene transcription. FAK has high expression levels in various human cancers as it promotes tumour cell motility, matrix degradation, angiogenesis and suppresses apoptosis, anoikis and senescence.

EX-527

EX-527__SIRT1 inhibitor AZD-8055

Product Name EX-527
Description

SIRT1 inhibitor
Purity >98% (TLC); NMR (Conforms)
CAS No. 49843-98-3
Molecular Formula C13H13ClN2O
Molecular Weight 248.7
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble in 18 mg/ml DMSO or 10 mg/ml Ethanol
Source Synthetic
Appearance Light Yellow Solid
SMILES C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N
InChI InChI=1S/C13H13ClN2O/c14-7-4-5-11-10(6-7)8-2-1-3-9(13(15)17)12(8)16-11/h4-6,9,16H,1-3H2,(H2,15,17)
InChIKey FUZYTVDVLBBXDL-UHFFFAOYSA-N
Safety Phrases Classification: Harmful- May be harmful if inhaled, swallowed, or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
Hazard Statements:
H301 – Toxic if swallowed
H319 – Causes serious eye irritation
Precautionary Statements:
P301 + P310 – If swallowed: Immediately call a POSION Center
P305 + P351 + P338: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Cite This Product EX-527 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-353)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19123978

Alternative Names 6-Chloro-2,3,4,9-tetrahydro-1Hcarbazole-1-carboxamide (racemic)
Research Areas Cell Signaling, Epigenetics
PubChem ID 5113032
Scientific Background Selective SIRT1 inhibitor (IC50=98 nM). Does not inhibit other HDACs or SIRT family members. Increases p53 acetylation following DNA damage. Cell permeable.
References 1. Solomon J.M., et al., (2006) Mol. Cell. Biol. 26: 28.
2. Anderson J.L., et al., (2011) Mol.Cell 43: 834.

Etoposide

Etoposide__Topoisomerase II inhibitor Regorafenib

Product Name Etoposide
Description

Topoisomerase II inhibitor
Purity >98%
CAS No. 33419-42-0
Molecular Formula C29H32O13
Molecular Weight 588.56
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inducer
Solubility Soluble to 100 mM in DMSO
Source Synthetic
Appearance White solid
SMILES CC@@H1OCC@@H2C@@H(O1)C@@H(email protected(C@@H(O2)Oemail protected3email protected4COC(=O)C@@H4C@@H(C5=CC6=C(C=C35)OCO6)C7=CC(=C(C(=C7)OC)O)OC)O)O
InChI InChI=1S/C29H32O13/c1-11-36-9-20-27(40-11)24(31)25(32)29(41-20)42-26-14-7-17-16(38-10-39-17)6-13(14)21(22-15(26)8-37-28(22)33)12-4-18(34-2)23(30)1
InChIKey VJJPUSNTGOMMGY-MRAJQDPKSA-N
Safety Phrases Classification: Toxic. May be harmful or fatal if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S53 – Avoid exposure – obtain special instruction before use
Risk Phrases:
R20/21/22 – Harmful by inhilation, in contact with skin and if swallowed
R45 – May cause cancer
R62 – Possible risk of impaired fertility
R68 – Possible risk of irreversible effects
Hazard Phrases:
H302-H350
Precautionary Phrases:
P201-P308 + P313
Cite This Product Etoposide (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-244)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19123941

Alternative Names (5S,5aR,8aR,9R)-9-(4-Hydroxy-3,5-dimethoxyphenyl)-8-oxo-5,5a,6,8,8a,9-hexahydrofuro3',4':6,7naphtho2,3-d1,3dioxol-5-yl 4,6-O-(1R)-ethylidene-β-D-glucopyranoside
Research Areas Cancer, Apoptosis
PubChem ID 36462
Scientific Background Etoposide inhibits the enzyme topoisomerase II, which unwinds DNA, and be doing so causes the DNA strand to break. Cancer cells are then less likely to be able to repair this damage (1).
References 1. Gordaliza M., et al. (2004) Toxicon. 44(4): 441-459.

Anti-ESR1 Antibody

Anti-ESR1 Antibody__Rabbit Anti-Human ESR1 Polyclonal LXR-623

Product Name ESR1 Antibody
Description

Rabbit Anti-Human ESR1 Polyclonal
Species Reactivity Human
Applications ,
WB
,
ICC/IF
Antibody Dilution WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthetic peptide from the mid-protein of Human ESR1
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects ~66 kDa.
Cite This Product Rabbit Anti-Human ESR1 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-708)
Certificate of Analysis A 1:1000 dilution of SPC-708 was sufficient for detection of ESR1 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19123923

Alternative Names Estrogen receptor alpha Antibody, ER alpha Antibody, ER-alpha Antibody, Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform Antibody, NR3A1 Antibody, Nuclear receptor subfamily 3 group A member 1 Antibody, Estrogen receptor alpha delta 4*,5,6,7*/654 isoform Antibody, ESR1_HUMAN Antibody, ESTRR Antibody, Era Antibody, Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform Antibody, Estrogen receptor 1 Antibody, Estrogen receptor alpha 3*,4,5,6,7*/822 isoform Antibody, DKFZp686N23123 Antibody, Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform Antibody, Estrogen receptor Antibody, Estrogen receptor alpha E1 E2 1 2 Antibody, ESR1 Antibody, Estrogen receptor alpha E1 N2 E2 1 2 Antibody, Estrogen nuclear receptor alpha Antibody, ESR Antibody, ER Antibody, ESRA Antibody, Estrogen receptor alpha delta 4 +49 isoform Antibody, Estradiol receptor Antibody
Research Areas Cancer, Cell Signaling, Domain Families, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, Receptors, Transcription, Tumor Biomarkers, Zinc finger
Cellular Localization Cytoplasm, Cell membrane, Cytoplasmic Side, Nucleus, Peripheral membrane protein
Accession Number NP_000116.2
Gene ID 2099
Swiss Prot P03372

Erlotinib Hydrochloride

Erlotinib Hydrochloride__EGFR Kinase inhibitor Tadalafil

Product Name Erlotinib Hydrochloride
Description

EGFR Kinase inhibitor
Purity >99%
CAS No. 183319-69-9
Molecular Formula C22H24ClN3O4
Molecular Weight 429.9
Storage Temperature -20ºC
Shipping Temperature Shipped Ambient
Product Type Inhibitor
Solubility Soluble in DMSO at 18 mg/ml with warming; very poorly soluble in ethanol; very poorly soluble in water; maximum solubility in plain water is estimated to be about 5-20 µM; buffers, serum, or other additives may increase or decrease the aqueous solubility
Source Synthetic
Appearance Solid powder
SMILES H+.C1=C(OCCOC)C(=CC2=NC=NC(=C12)NC3=CC(=CC=C3)C#C)OCCOC.Cl-
InChI InChI=1S/C22H23N3O4.ClH/c1-4-16-6-5-7-17(12-16)25-22-18-13-20(28-10-8-26-2)21(29-11-9-27-3)14-19(18)23-15-24-22;/h1,5-7,12-15H,8-11H2,2-3H3,(H,23,24,25);1H
InChIKey GTTBEUCJPZQMDZ-UHFFFAOYSA-N
Safety Phrases Classification:
Not a hazardous substance or mixture.

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Cite This Product Erlotinib Hydrochloride (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-444)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19123864

Alternative Names N-(3-ethynylphenyl)-6,7-bis(2-methoxyethoxy)quinazolin-4-amine hydrochloride
Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors
PubChem ID 176871
Scientific Background Erlotinib Hydrochloride inhibits the human epidermal growth factor receptor (HER-1/EGFR) tyrosine kinase.
References 1. Ali S., et al. (2008) Mol. Cancer Ther. 7(6): 1708–1719.