Anti-FGFR1 Antibody__Rabbit Anti-Human FGFR1 Polyclonal CP-673451
Anti-FGFR1 Antibody
Anti-FGFR1 Antibody__Rabbit Anti-Human FGFR1 Polyclonal BMS-303141
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 91.868 (120-145) kDa.
Cite This Product
Rabbit Anti-Human FGFR1 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1146)
Certificate of Analysis
A 1:250 dilution of SPC-1146 was sufficient for detection of FGFR1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124169
Alternative Names
Basic fibroblast growth factor receptor 1 Antibody, bFGF-R-1 Antibody, BFGFR Antibody, FGFR1 Antibody, CD331 Antibody, CEK Antibody, FGFBR Antibody, FGFR1 Antibody, FGFR1/PLAG1 fusion Antibody, fibroblast growth factor receptor 1 Antibody, FLG Antibody, FLT2 Antibody, Fms-like tyrosine kinase 2 Antibody, HBGFR Antibody, heparin-binding growth factor receptor Antibody, HH2 Antibody, HRTFDS Antibody, hydroxyaryl-protein kinase Antibody, KAL2 Antibody, N-SAM Antibody, OGD Antibody, Proto-oncogene c-Fgr Antibody
Cellular Localization
Cytoplasm, Cell membrane, Cytoplasmic Vesicle, Cytosol, Nucleus
Accession Number
NP_075598
Gene ID
2260
Swiss Prot
P11362
Scientific Background
FGFR1 is a receptor-tyrosine kinase that is activated upon binding fibroblast growth factor (FGF). It is a key player in many numerous cell signalling pathways including MAPK/AKT/PLC/STAT/NF-kB. FGF and the FGFR1 is a stimulator of cell differentiation, proliferation, repair, angiogenesis, pro-survival, anti-apoptosis, etc. Overexpression leads to disregulated cell growth which forms the basis of early oncogenesis. This occurs through amplification, gene duplication, or translocation leading to constitutive activation of the receptor.
Anti-FES Antibody (pTyr713)
Anti-FES Antibody (pTyr713)__Rabbit Anti-Human FES (pTyr713) Polyclonal CY5-SE
Product Name
FES Antibody (pTyr713)
Description
Rabbit Anti-Human FES (pTyr713) Polyclonal
Species Reactivity
Human
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr713 of human FES (AA710-716)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa
Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet
Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:
Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:
Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:
Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:
Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:
Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:
Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol
Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:
Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:
Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:
Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:
Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 93.471 kDa.
Cite This Product
Rabbit Anti-Human FES (pTyr713) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-979)
Certificate of Analysis
A 1:250 dilution of SPC-979 was sufficient for detection of FES (pTyr713) in 10 µg of human placenta lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124113
Alternative Names
FPS Antibody, c-fes/fps protein Antibody, cFES Antibody, FES Antibody, Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog Antibody, feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami Antibody, Feline sarcoma oncogene Antibody, FES proto oncogene tyrosine kinase Antibody, FES_HUMAN Antibody, Oncogene FES Antibody, P93c fes Antibody, Proto oncogene tyrosine protein kinase, Fes/Fps Antibody, Proto-oncogene c-Fes Antibody, Proto-oncogene c-Fps Antibody, Tyrosine-protein kinase Fes/Fps Antibody
Cellular Localization
Cytoplasm, Cell Junctions, Cell membrane, Cytosol, Focal Adhesion, Golgi apparatus
Accession Number
NP_001996
Gene ID
2242
Swiss Prot
P07332
Scientific Background
FES is a protein-tyrosine kinase is expressed in macrophages and is thought to be involved in the regulation of myeloid differentiation. Fes has several characteristics typical of a cytoplasmic class of protein tyrosine kinases, including an SH2 domain and autophosphorylation capabilities. Fes has been shown to associate with IL-4 and several hematopoietic cytokine receptors, as well as with BCR. Phosphorylation of BCR by Fes induces the association of BCR with the Ras guanine nucleotide exchange factor complex GRB2/Sos.
Rabbit Anti-Human FES (pTyr713) Polyclonal
WB
,
AM
| APC (Allophycocyanin) | ||
Overview:
|
|
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
| ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
|
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
| ATTO 488 | ||
| Overview: | |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
| ATTO 565 | ||
| Overview: | |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
| ATTO 594 | ||
| Overview: | |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
| ATTO 633 | ||
| Overview: | |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
| ATTO 655 | ||
| Overview: | |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
| ATTO 680 | ||
| Overview: | |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
| ATTO 700 | ||
Overview:
|
|
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
| FITC (Fluorescein) | ||
| Overview: | |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
| PE/ATTO 594 | ||
| PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
| Overview: | |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
| PerCP | ||
| Overview: | |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
| R-PE (R-Phycoerythrin) | ||
| Overview: | |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Anti-FES Antibody (pTyr713 + pSer716)
Anti-FES Antibody (pTyr713 + pSer716)__Rabbit Anti-Human FES (pTyr713 + pSer716) Polyclonal VX-809
Product Name
FES Antibody (pTyr713 + pSer716)
Description
Rabbit Anti-Human FES (pTyr713 + pSer716) Polyclonal
Species Reactivity
Human
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr713 and Ser716 of human FES (AA711-720)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa
Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet
Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:
Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:
Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:
Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:
Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:
Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:
Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol
Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:
Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:
Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:
Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:
Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 93.471 kDa.
Cite This Product
Rabbit Anti-Human FES (pTyr713 + pSer716) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-980)
Certificate of Analysis
A 1:250 dilution of SPC-980 was sufficient for detection of FES (pTyr713 + pSer716) in 10 µg of human placenta lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124109
Alternative Names
FPS Antibody, c-fes/fps protein Antibody, cFES Antibody, FES Antibody, Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog Antibody, feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami Antibody, Feline sarcoma oncogene Antibody, FES proto oncogene tyrosine kinase Antibody, FES_HUMAN Antibody, Oncogene FES Antibody, P93c fes Antibody, Proto oncogene tyrosine protein kinase, Fes/Fps Antibody, Proto-oncogene c-Fes Antibody, Proto-oncogene c-Fps Antibody, Tyrosine-protein kinase Fes/Fps Antibody
Cellular Localization
Cytoplasm, Cell Junctions, Cell membrane, Cytosol, Focal Adhesion, Golgi apparatus
Accession Number
NP_001996
Gene ID
2243
Swiss Prot
P07332
Scientific Background
FES is a protein-tyrosine kinase is expressed in macrophages and is thought to be involved in the regulation of myeloid differentiation. Fes has several characteristics typical of a cytoplasmic class of protein tyrosine kinases, including an SH2 domain and autophosphorylation capabilities. Fes has been shown to associate with IL-4 and several hematopoietic cytokine receptors, as well as with BCR. Phosphorylation of BCR by Fes induces the association of BCR with the Ras guanine nucleotide exchange factor complex GRB2/Sos.
Rabbit Anti-Human FES (pTyr713 + pSer716) Polyclonal
WB
,
AM
| APC (Allophycocyanin) | ||
Overview:
|
|
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
| ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
|
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
| ATTO 488 | ||
| Overview: | |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
| ATTO 565 | ||
| Overview: | |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
| ATTO 594 | ||
| Overview: | |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
| ATTO 633 | ||
| Overview: | |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
| ATTO 655 | ||
| Overview: | |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
| ATTO 680 | ||
| Overview: | |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
| ATTO 700 | ||
Overview:
|
|
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
| FITC (Fluorescein) | ||
| Overview: | |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
| PE/ATTO 594 | ||
| PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
| Overview: | |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
| PerCP | ||
| Overview: | |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
| R-PE (R-Phycoerythrin) | ||
| Overview: | |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Ferrostatin-1
Ferrostatin-1__Ferroptosis Inhibitor SAR405838
Product Name
Ferrostatin-1
Description
Ferroptosis Inhibitor
Purity
>98% (TLC); NMR (Conforms)
CAS No.
347174-05-4
Molecular Formula
C15H22N2O2
Molecular Weight
262.4
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in DMSO (100 mg/ml) or ethanol (100 mg/ml)
Source
Synthetic
Appearance
Tan powder
SMILES
CCOC(=O)c1ccc(c(c1)N)NC2CCCCC2
Safety Phrases
Classification:
Skin irritation (Category 2), H315
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H315 Causes skin irritation.
H319 Causes serious eye irritation.
Precautionary statements:
P260 Do not breathe dust/fume/gas/mist/vapours/spray P264 Wash skin thoroughly after handling.
P280 Wear protective gloves.
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + 340 If INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
P305 + 351+ + 338 IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do so- continue rinsing.
P333 + 313 If skin irritation or a rash occurs: Get medical advice/attention
Cite This Product
Ferrostatin-1 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-525)
Alternative Names
Ethyl 3-amino-4-(cyclohexylamino)benzoate
Research Areas
Cancer, Apoptosis, Cell Signaling, Neuroscience
Scientific Background
Ferrostatin-1 inhibits ferroptosis (EC50=60 nM), an iron-dependent form of nonapoptotic cell death. It is especially potent of ferroptosis in cancer cells and glutamate-induced cell death in organotypic rat brain slices (1). It also blocks the cytotoxic effects of sorafenib in hepatocellular carcinoma cells (2), and inhibits oxidative lipid damage and cell death in diverse disease models (3).
References
1. Dixon S.J., et al. (2012) Cell. 149: 1060.
2. Louandre C., et al. (2013) Int. J. Cancer. 133: 1732.
3. Skouta R., et al. (2014) J. Am. Chem. Soc. 136: 4551.
Ferroptosis Inhibitor
Skin irritation (Category 2), H315
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H315 Causes skin irritation.
H319 Causes serious eye irritation.
Precautionary statements:
P260 Do not breathe dust/fume/gas/mist/vapours/spray P264 Wash skin thoroughly after handling.
P280 Wear protective gloves.
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + 340 If INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
P305 + 351+ + 338 IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do so- continue rinsing.
P333 + 313 If skin irritation or a rash occurs: Get medical advice/attention
2. Louandre C., et al. (2013) Int. J. Cancer. 133: 1732.
3. Skouta R., et al. (2014) J. Am. Chem. Soc. 136: 4551.
Felodipine
Felodipine__L-type Ca channel blocker Celastrol
Product Name
Felodipine
Description
L-type Ca channel blocker
Purity
>98% (TLC); NMR (Conforms)
CAS No.
72509-76-3
Molecular Formula
C18H19Cl2NO4
Molecular Weight
384.25
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble to 100 mM in ethanol and to 100 mM in DMSO
Source
Synthetic
Appearance
Light Yellow Solid
SMILES
CCOC(=O)C1=C(NC(=C(C1C2=C(C(=CC=C2)Cl)Cl)C(=O)OC)C)C
InChI
InChI=1S/C18H19Cl2NO4/c1-5-25-18(23)14-10(3)21-9(2)13(17(22)24-4)15(14)11-7-6-8-12(19)16(11)20/h6-8,15,21H,5H2,1-4H3
InChIKey
RZTAMFZIAATZDJ-UHFFFAOYSA-N
Safety Phrases
Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with shin and eyes
Risk Phrases:
R20/21/22 – Harmful by inhilation, in contact with skin and if swallowed
R62 – Possible risk of impaired fertility
Hazard Phrases:
H302
Cite This Product
Felodipine (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-323)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124046
Alternative Names
Ethyl methyl 4-(2,3-dichlorophenyl)-2,6-dimethyl-1,4-dihydro-3,5-pyridinedicarboxylate, Plendil
Research Areas
Calcium Channels, Ion Channels, Neuroscience, Voltage-Gated Calcium Channels
PubChem ID
3333
Scientific Background
Felodipine is an L-type Ca2+ channel blocker (1). In isolated rat ventricular myocytes, using a whole-cell patch-clamp technique, it displays a Ki of 11 nM (2). It is a clinically useful antihypertensive agent (3) which attenuates the hypertrophic effect of cyclosporine on transplanted hearts (4).
References
1. Furakawa T., et al. (2005) J Cardiovasc. Pharmacol. 45(3): 241-246.
2. Zahradnikova Z., Minarovic I., Zahradnik I. (2007) J Pharmacol Exp. Ther. 322(2): 638-645.
3. Manzo B.A., Matalka M.S., Ravnan S.L. (2003) Pharmacotherapy. 23(11): 1508-1512.
4. Schwitter J., et al. (1999) J Heart Lung Transplant. 18(10): 1003-1013.
L-type Ca channel blocker
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with shin and eyes
Risk Phrases:
R20/21/22 – Harmful by inhilation, in contact with skin and if swallowed
R62 – Possible risk of impaired fertility
Hazard Phrases:
H302
2. Zahradnikova Z., Minarovic I., Zahradnik I. (2007) J Pharmacol Exp. Ther. 322(2): 638-645.
3. Manzo B.A., Matalka M.S., Ravnan S.L. (2003) Pharmacotherapy. 23(11): 1508-1512.
4. Schwitter J., et al. (1999) J Heart Lung Transplant. 18(10): 1003-1013.
FCR1
FCR1__FRET ratiometric fluorescence-based redox sensor GSK1324726A
Product Name
FCR1
Description
FRET ratiometric fluorescence-based redox sensor
Molecular Formula
C34H37N7O6
Molecular Weight
639
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Product Type
Redox Probe
Solubility
Soluble in DMSO
Source
Synthetic
Appearance
Orange Solid
Safety Phrases
Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Cite This Product
FCR1 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-180)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124024/
Alternative Names
7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzogpteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide (FCR1)
Research Areas
Cancer, Oxidative Stress
Scientific Background
FCR1 or flavin coumarin redox sensor 1, is a novel ratiometric fluorescent redox sensor utilized in fluorescence lifetime imaging microscopy and flow cytometry. In the oxidized form, excitation of FCR1 at 405nm results in a green fluorescence with max emissions at 525nm. Treatment with a mild reducing agent (including sodium cyanoborohydride, DTT, and glutathione) reduces the flavin thereby decreasing the green fluorescence intensity, and increasing the blue. Re-oxidation can be achieved using mild oxidizing agents. In general, the FCR1 probe can be used to observe changes in oxidative capacity without interference from background effects.
References
1. Kaur A., Haghighatbin M.A., Hogan C.F., and New E.J. (2015) Chem. Commun. Epub.
FRET ratiometric fluorescence-based redox sensor
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
FCCP
FCCP__MPTP inhibitor GANT 61
Product Name
FCCP
Description
MPTP inhibitor
Purity
>98%
CAS No.
370-86-5
Molecular Formula
C10H5F3N4O
Molecular Weight
254.17
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inducer
Solubility
Soluble to 100 mM in DMSO
Source
Synthetic
Appearance
Yellow solid
SMILES
C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F
InChI
InChI=1S/C2H6FP/c3-1-2-4/h1-2,4H2
InChIKey
GYQDBNYYNSTQTM-UHFFFAOYSA-N
Safety Phrases
Classification: Toxic. May be harmful or fatal if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R23/24/25- Toxic by inhalation, in contact with skin, and if swallowed
Hazard Phrases:
H301-H317-H319-H413
Precautionary Phrases:
P280-P301 + P310-P305 + P351 + P338
Cite This Product
FCCP (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-222)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124024
Alternative Names
{4-(Trifluoromethoxy)phenylhydrazono}malononitrile
Research Areas
Cancer, Apoptosis
PubChem ID
3330
Scientific Background
FCCP is a tool for inhibiting mitochondrial oxidative phosphorylation (1, 2).
References
1. Collins T.J., et al. (2000) Biochem J. 347: 593.
2. Keij J.F., et al. (2000) Cytometry 39: 203.
MPTP inhibitor
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R23/24/25- Toxic by inhalation, in contact with skin, and if swallowed
Hazard Phrases:
H301-H317-H319-H413
Precautionary Phrases:
P280-P301 + P310-P305 + P351 + P338
2. Keij J.F., et al. (2000) Cytometry 39: 203.
Fasudil
Fasudil__ROCK inhibitor STING agonist-1
Product Name
Fasudil
Description
ROCK inhibitor
Purity
>98% (TLC); NMR (Conforms)
CAS No.
103745-39-7
Molecular Formula
C14H17N3O2S•HCl
Molecular Weight
327.83
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
May be dissolved in DMSO (75 mg/ml) or water (100 mg/ml)
Source
Synthetic
Appearance
Powder
SMILES
C1CNCCN(C1)S(=O)(=O)C2=CC=CC3=C2C=CN=C3
InChI
InChI=1S/C14H17N3O2S/c18-20(19,17-9-2-6-15-8-10-17)14-4-1-3-12-11-16-7-5-13(12)14/h1,3-5,7,11,15H,2,6,8-10H2
InChIKey
NGOGFTYYXHNFQH-UHFFFAOYSA-N
Safety Phrases
Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Cite This Product
Fasudil (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-371)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124014
Alternative Names
1-(5-Isoquinolinylsulfonyl) homopiperazine hydrochloride; 5-(1,4-Diazepan-1-ylsulfonyl)isoquinoline
Research Areas
Cell Signaling, Neuroscience
PubChem ID
3547
Scientific Background
Fasudil is a ROCK2 inhibitor with some action on PRK2 and MSK1 (IC50 values are 1.9 µM, 4 µM and 5 µM respectively). It is also shown to inhibit RSK1, RSK2, PKA, AMPK and PKd1. It is also a calcium channel antagonist and vasodilator.
References
1. Shirotani M., et al. (1991) J. Pharmacol. Exp. Ther. 259(2): 738-744.
2. Arai M., Nozawa R., et al. (1993) Biochem. Pharmacol. 46(8): 1487-1490.
3. Satoh S., et al. (1996) Br. J. Pharmacol. 118(7): 1592-1606.
4. Sward K., et al. (2000) J. Physiol. 522: 33-49.
ROCK inhibitor
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
2. Arai M., Nozawa R., et al. (1993) Biochem. Pharmacol. 46(8): 1487-1490.
3. Satoh S., et al. (1996) Br. J. Pharmacol. 118(7): 1592-1606.
4. Sward K., et al. (2000) J. Physiol. 522: 33-49.
Anti-FAK Antibody (pTyr577)
Anti-FAK Antibody (pTyr577)__Rabbit Anti-Human FAK (pTyr577) Polyclonal Iopamidol
Product Name
FAK Antibody (pTyr577)
Description
Rabbit Anti-Human FAK (pTyr577) Polyclonal
Species Reactivity
Human
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr577 of human FAK (AA574-580)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa
Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet
Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:
Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:
Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:
Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:
Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:
Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:
Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol
Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:
Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:
Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:
Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:
Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 119.233 kDa.
Cite This Product
Rabbit Anti-Human FAK (pTyr577) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-978)
Certificate of Analysis
A 1:250 dilution of SPC-978 was sufficient for detection of FAK (pTyr577) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19124005
Alternative Names
FADK1 Antibody, FADK Antibody, FAK related non kinase polypeptide Antibody, FAK1 Antibody, FAK Antibody, Focal adhesion kinase 1 Antibody, Focal adhesion kinase isoform FAK Del33 Antibody, Focal adhesion kinase related nonkinase Antibody, FRNK Antibody, p125FAK Antibody, PPP1R71 Antibody, Protein phosphatase 1 regulatory subunit 71 Antibody, Protein tyrosine kinase 2 Antibody, Ptk2 Antibody, PTK2 protein tyrosine kinase 2 Antibody
Cellular Localization
Cell Junctions, Cell membrane, Focal Adhesion
Accession Number
NP_722560
Gene ID
5747
Swiss Prot
Q05397
Scientific Background
FAK, also known as PTK2, is a non-receptor protein-tyrosine kinase that is activated by tyrosine-phosphorylation in response to integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands. It serves as adaptor protein. It is linked to the MAPK pathway via Grb2, and is also linked to PI3K and PLC gamma pathways. It plays a role as a scaffold for MDM2-mediated p53 degradation, and has been implicated in regulation of cell-cell contacts, microtubule stability and control of gene transcription. FAK has high expression levels in various human cancers as it promotes tumour cell motility, matrix degradation, angiogenesis and suppresses apoptosis, anoikis and senescence.
Rabbit Anti-Human FAK (pTyr577) Polyclonal
WB
,
AM
| APC (Allophycocyanin) | ||
Overview:
|
|
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
| ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
|
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
| ATTO 488 | ||
| Overview: | |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
| ATTO 565 | ||
| Overview: | |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
| ATTO 594 | ||
| Overview: | |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
| ATTO 633 | ||
| Overview: | |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
| ATTO 655 | ||
| Overview: | |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
| ATTO 680 | ||
| Overview: | |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
| ATTO 700 | ||
Overview:
|
|
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
| FITC (Fluorescein) | ||
| Overview: | |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
| PE/ATTO 594 | ||
| PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
| Overview: | |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
| PerCP | ||
| Overview: | |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
| R-PE (R-Phycoerythrin) | ||
| Overview: | |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA