Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1__Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1 Roscovitine
Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1
Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1__Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1 AMG-337
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Protein G Purified
Clonality
Monoclonal
Clone Number
6G3
Isotype
IgG1
Specificity
Specific for 3,5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine.
Cite This Product
Mouse Anti-Dibromo-tyrosine Monoclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SMC-506)
Certificate of Analysis
A 1:1000 dilution of SMC-506 was sufficient for detection of 3,5-Dibromotyrosine in 1 µg of 3, 5-Dibromotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120475
Alternative Names
3,5-Dibromo-tyrosine Antibody, 3,5-dibromotyrosine Antibody, Dibromotyrosine Antibody, Dibromo-tyrosine Antibody, DiBrY Antibody, Dibromotyrosine (DiBrY) Antibody, Dibromo-Tyrosine (DiBrY) Antibody, Dibromo-tyrosine DiBrY Antibody
Research Areas
Cancer, Cell Signaling, Oxidation, Oxidative Stress, Post-translational Modifications, Protein Oxidation
Diazoxide
Diazoxide__K-ATP channel activator AS-605240
Product Name
Diazoxide
Description
K-ATP channel activator
Purity
>98% (HPLC); NMR (conforms)
CAS No.
364-98-7
Molecular Formula
C8H7ClN2O2S
Molecular Weight
230.7
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Product Type
Activator
Solubility
May be dissolved in DMSO (20 mg/ml)
Source
Synthetic
Appearance
White powder
SMILES
CC1=NS(=O)(=O)C2=C(N1)C=CC(=C2)Cl
InChI
InChI=1S/C8H7ClN2O2S/c1-5-10-7-3-2-6(9)4-8(7)14(12,13)11-5/h2-4H,1H3,(H,10,11)
InChIKey
GDLBFKVLRPITMI-UHFFFAOYSA-N
Safety Phrases
WHMIS Classification:
D2B Toxic Material Causing Other Toxic – Effects Moderate skin irritant, Moderate respiratory irritant, Moderate eye irritant
GHS Classification:
Acute toxicity, Oral (Category 4)
Skin irritation (Category 2)
Eye irritation (Category 2A)
Specific target organ toxicity – single exposure (Category 3)
Signal word: Warning
Hazard statement(s):
H302 Harmful if swallowed.
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H335 May cause respiratory irritation.
Precautionary statement(s):
P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264 Wash skin thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P271 Use only outdoors or in a well-ventilated area.
P280 Wear protective gloves/ eye protection/ face protection.
P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312 Call a POISON CENTER or doctor/ physician if you feel unwell.
P321 Specific treatment (see supplemental first aid instructions on this label).
P330 Rinse mouth.
P332 + P313 If skin irritation occurs: Get medical advice/ attention.
P337 + P313 If eye irritation persists: Get medical advice/ attention.
P362 Take off contaminated clothing and wash before reuse.
P403 + P233 Store in a well-ventilated place. Keep container tightly closed.
P405 Store locked up.
P501 Dispose of contents/ container to an approved waste disposal plant.
Cite This Product
Diazoxide (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-548)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120359
Alternative Names
7-chloro-3-methyl-4H-1$l^{6},2,4-benzothiadiazine 1,1-dioxide
Research Areas
Ion Channels, Neuroscience, Potassium Channels
PubChem ID
3019
K-ATP channel activator
D2B Toxic Material Causing Other Toxic – Effects Moderate skin irritant, Moderate respiratory irritant, Moderate eye irritant
GHS Classification:
Acute toxicity, Oral (Category 4)
Skin irritation (Category 2)
Eye irritation (Category 2A)
Specific target organ toxicity – single exposure (Category 3)
Signal word: Warning
Hazard statement(s):
H302 Harmful if swallowed.
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H335 May cause respiratory irritation.
Precautionary statement(s):
P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264 Wash skin thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P271 Use only outdoors or in a well-ventilated area.
P280 Wear protective gloves/ eye protection/ face protection.
P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312 Call a POISON CENTER or doctor/ physician if you feel unwell.
P321 Specific treatment (see supplemental first aid instructions on this label).
P330 Rinse mouth.
P332 + P313 If skin irritation occurs: Get medical advice/ attention.
P337 + P313 If eye irritation persists: Get medical advice/ attention.
P362 Take off contaminated clothing and wash before reuse.
P403 + P233 Store in a well-ventilated place. Keep container tightly closed.
P405 Store locked up.
P501 Dispose of contents/ container to an approved waste disposal plant.
Dexamethasone
Dexamethasone__Glucocorticoid receptor ligand Maytansinol
Product Name
Dexamethasone
Description
Glucocorticoid receptor ligand
Purity
>98%
CAS No.
50-02-2
Molecular Formula
C22H29FO5
Molecular Weight
392.47
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inducer
Solubility
Soluble to 100 mM in DMSO
Source
Synthetic
Appearance
White solid
SMILES
CC@@H1Cemail protected2C@@H3CCC4=CC(=O)C=CC@@4(C@3(email protected(CC@@2(C@1(C(=O)CO)O)C)O)F)C
InChI
InChI=1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,2
InChIKey
UREBDLICKHMUKA-CXSFZGCWSA-N
Safety Phrases
Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R62 – Possible risk of impaired fertility
R68- Possible risk of irreversible effects
Hazard Phrases:
H315-H317-H319-H334-H335
Precautionary Phrases:
P261-P280-P305 + P351 + P338-P342 + P311
Cite This Product
Dexamethasone (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-259)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120339
Alternative Names
(11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione
Research Areas
Cancer, Apoptosis
PubChem ID
5743
Scientific Background
Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs. It is an anti-inflammatory and immunosuppressant. In cancer patients, it can be give to augment the antiemetic effect of 5-HT3 receptor antagonists, and is also used to counteract the development of edema (1). In a diagnostic setting, it is used to suppress the natural pituitary-adrenal axis (2).
References
1. Roila F., and Fatigoni S. (2006) Ann Oncol.17 (Suppl2): ii96-ii100.
2. Hasan E.A., Jessop D.S., Power L.L. Monk P.T. and Kirwan J.R. (2009) Int J Endocrinol. 2009: 391284.
Glucocorticoid receptor ligand
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R62 – Possible risk of impaired fertility
R68- Possible risk of irreversible effects
Hazard Phrases:
H315-H317-H319-H334-H335
Precautionary Phrases:
P261-P280-P305 + P351 + P338-P342 + P311
2. Hasan E.A., Jessop D.S., Power L.L. Monk P.T. and Kirwan J.R. (2009) Int J Endocrinol. 2009: 391284.
Deprenyl
Deprenyl__Apoptosis Inhibitor ICG-001
Product Name
Deprenyl
Description
Apoptosis Inhibitor
Purity
>99%
CAS No.
14611-52-0
Molecular Formula
C13H17N·HCl
Molecular Weight
223.74
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble to 100 mM in water, and to 100 mM in ethanol
Source
Synthetic
Appearance
White Solid
SMILES
Cemail protected(CC1=CC=CC=C1)N(C)CC#C
InChI
InChI=1S/C13H17N.ClH/c1-4-10-14(3)12(2)11-13-8-6-5-7-9-13;/h1,5-9,12H,10-11H2,2-3H3;1H/t12-;/m1./s1
InChIKey
IYETZZCWLLUHIJ-UTONKHPSSA-N
Safety Phrases
Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R62- Possible risk of impaired fertility
Cite This Product
Deprenyl (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-209)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120324
Alternative Names
N-Methyl-N-(2R)-1-phenyl-2-propanyl-2-propyn-1-amine, Selegiline
Research Areas
Cancer, Apoptosis
PubChem ID
26757
Scientific Background
Deprenyl or Selegiline is a drug used for the treatment of early stage Parkinson’s (1), depression and senile dementia (2). It is a selective irreversable inhibitor of monoamine oxidase B (MAO-b).
References
1. Lachenmayer L., and Laux G. (2004) Curr Med Chem. 11(15): 2033-2043.
2. FDA US Food and Drug Administration. Press Announcements Feb 28, 2006. www.fda.gov/newsevents/newsroom/pressannouncements/2006/ucm108607.htm
Apoptosis Inhibitor
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Risk Phrases:
R62- Possible risk of impaired fertility
2. FDA US Food and Drug Administration. Press Announcements Feb 28, 2006. www.fda.gov/newsevents/newsroom/pressannouncements/2006/ucm108607.htm
Deguelin
Deguelin__Akt inhibitor GW 4064
Product Name
Deguelin
Description
Akt inhibitor
Purity
>98%
CAS No.
522-17-8
Molecular Formula
C23H22O6
Molecular Weight
394.42
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inducer
Solubility
Soluble to 50 mM in ethanol and to 100 mM in DMSO
Source
Synthetic
Appearance
Cream Solid
SMILES
CC1(C=CC2=C(O1)C=CC3=C2OC@@H4COC5=CC(=C(C=C5C@@H4C3=O)OC)OC)C
InChI
InChI=1S/C23H22O6/c1-23(2)8-7-12-15(29-23)6-5-13-21(24)20-14-9-17(25-3)18(26-4)10-16(14)27-11-19(20)28-22(12)13/h5-10,19-20H
InChIKey
ORDAZKGHSNRHTD-UXHICEINSA-N
Safety Phrases
Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Cite This Product
Deguelin (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-251)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120321
Alternative Names
(7aS,13aS)-9,10-Dimethoxy-3,3-dimethyl-13,13a-dihydro-3H-chromeno3,4-bpyrano2,3-hchromen-7(7aH)-one
Research Areas
Cancer, Apoptosis
PubChem ID
107935
Scientific Background
Deguelin is a rotenoid, and a naturally occurring insecticide. Deguelin displays anti-cancer activity by inhibiting the growth of pre-cancerous and cancerous cells (1). High doses may have negative effects on the heart, lungs and nerves however. Deguelin has also been shown to have a correlation with Parkinson’s disease in rats (2).
References
1. Lee H.Y. (2004) Biochem Pharmacol. 68(6): 1119-1124.
2. Caboni P., et al. (2004) Chem Res. Toxicol. 17(11): 1540-1548.
Akt inhibitor
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
2. Caboni P., et al. (2004) Chem Res. Toxicol. 17(11): 1540-1548.
Decitabine
Decitabine__DNA hypomethylation agent Iguratimod
Product Name
Decitabine
Description
DNA hypomethylation agent
Purity
>98% (TLC); NMR (Conforms)
CAS No.
2353-33-5
Molecular Formula
C8H12N4O4
Molecular Weight
228.2
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in 25 mg/ml DMSO, 11 mg/ml Water or Ethanol
Source
Synthetic
Appearance
White Solid
SMILES
C1C@@H(email protected(Oemail protected1N2C=NC(=NC2=O)N)CO)O
InChI
InChI=1S/C8H12N4O4/c9-7-10-3-12(8(15)11-7)6-1-4(14)5(2-13)16-6/h3-6,13-14H,1-2H2,(H2,9,11,15)/t4-,5+,6+/m0/s1
InChIKey
XAUDJQYHKZQPEU-KVQBGUIXSA-N
Safety Phrases
Classification: Harmful – May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
Hazard Statements:
H341 – Suspected of causing genetic defects
H360 – May damage fertility or the unborn child
Precautionary Statements:
P280 – Wear protective gloves/protective clothing/ eye protection/ face protection
P308+313 – If exposed or concerned: Get Medical Advise/Attention
Cite This Product
Decitabine (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-352)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120312
Alternative Names
5-Aza-2’-deoxycytidine; NSC-127716, 2'-Deoxy-5-azacytidine; 4-Amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,3,5-triazin-2(1H)-one
Research Areas
Cancer, Cell Signaling, Epigenetics
PubChem ID
451668
Scientific Background
Inhibits DNA methyltransferase. Induces demethylation and reactivation of silenced genes. Increases stem cell reprogramming efficiency. Stimulates osteogenic differentiation of human bone marrow stromal cells. Antitumor agent. Cell permeable.
References
1. Bender C.M., et al. (1998) Cancer Res. 58: 95.
2. El-Serafi A.T., et al. (2011) Differentiation 81: 35.
DNA hypomethylation agent
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
Hazard Statements:
H341 – Suspected of causing genetic defects
H360 – May damage fertility or the unborn child
Precautionary Statements:
P280 – Wear protective gloves/protective clothing/ eye protection/ face protection
P308+313 – If exposed or concerned: Get Medical Advise/Attention
2. El-Serafi A.T., et al. (2011) Differentiation 81: 35.
Anti-DDX4 Antibody
Anti-DDX4 Antibody__Rabbit Anti-Human DDX4 Polyclonal Everolimus
Product Name
DDX4 Antibody
Description
Rabbit Anti-Human DDX4 Polyclonal
Species Reactivity
Human, Mouse, Rat
Applications
,
WB
Antibody Dilution
WB (1:1000); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
Peptide sequence around aa.273~277(T-I-L-V-E)derived from Human DDX4.
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa

Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet

Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:

Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:

Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:

Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:

Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:

Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:

Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol

Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:

Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:

Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:

Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS, pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Affinity Purified
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous level of total DDX4 protein.
Cite This Product
Rabbit Anti-Human DDX4 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1322)
Certificate of Analysis
A 1:1000 dilution of SPC-1322 was sufficient for detection of DDX4 in 10 µg of HeLa, K562, 3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120285
Alternative Names
DDX 4 antibody, Ddx4 antibody, DDX4_HUMAN antibody, DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody, DEAD box protein 4 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody, MVH antibody, Probable ATP dependent RNA helicase DDX4 antibody, Probable ATP-dependent RNA helicase DDX4 antibody, VASA antibody, Vasa homolog antibody
Cellular Localization
Cytoplasm, Perinuclear Region
Accession Number
NP_077726.1
Gene ID
54514
Swiss Prot
Q9NQI0
Scientific Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.
References
1. Castrillon D.H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:9585-9590.
2. Schmutz J. et al. (2004) Nature 431:268-274.
Rabbit Anti-Human DDX4 Polyclonal
WB
APC (Allophycocyanin) | ||
Overview:
|
![]() |
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
![]() |
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
ATTO 488 | ||
Overview: | ![]() |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
ATTO 565 | ||
Overview: | ![]() |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
ATTO 594 | ||
Overview: | ![]() |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
ATTO 633 | ||
Overview: | ![]() |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
ATTO 655 | ||
Overview: | ![]() |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
ATTO 680 | ||
Overview: | ![]() |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
ATTO 700 | ||
Overview:
|
![]() |
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
FITC (Fluorescein) | ||
Overview: | ![]() |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
PE/ATTO 594 | ||
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
Overview: | ![]() |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
PerCP | ||
Overview: | ![]() |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
R-PE (R-Phycoerythrin) | ||
Overview: | ![]() |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
2. Schmutz J. et al. (2004) Nature 431:268-274.
DBeQ
DBeQ__p97 inhibitor Fexaramine
Product Name
DBeQ
Description
p97 inhibitor
Purity
>98%
Molecular Formula
C22H20N4
Molecular Weight
340.4
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in 25 mg/ml DMSO
Source
Synthetic
Appearance
Yellow Powder
SMILES
C1=CC=C(C=C1)CNC2=NC(=NC3=CC=CC=C32)NCC4=CC=CC=C4
InChI
InChI=1S/C22H20N4/c1-3-9-17(10-4-1)15-23-21-19-13-7-8-14-20(19)25-22(26-21)24-16-18-11-5-2-6-12-18/h1-14H,15-16H2,(H2,23,24,25,26)
InChIKey
QAIMUUJJAJBPCL-UHFFFAOYSA-N
Safety Phrases
Classification: Toxic Material Causing other toxic effects- moderate eye irritant.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
Hazard Statements:
H302 – Harmful if swallowed
Precautionary Statements:
P305 + P351 + P338 – If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Cite This Product
DBeQ (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-334)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120276
Alternative Names
N,N'-Dibenzyl-2,4-quinazolinediamine, JRF 12, N2,N4-dibenzylquinazoline-2,4-diamine, N2,N4-Bis(phenylmethyl)-2,4-quinazolinediamine
Research Areas
Cancer, Apoptosis
PubChem ID
676352
Scientific Background
A potent, selective and reversible inhibitor of the AAA-ATP p97 (ATPase associated with diverse cellular activities). Ki=3.2 M. It blocks ubiquitin-dependent protein clearance pathways. Cell permeable.
References
1. Chou T.F., et al. 2011 PNAS 108:4834.
p97 inhibitor
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
Hazard Statements:
H302 – Harmful if swallowed
Precautionary Statements:
P305 + P351 + P338 – If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Dasatinib
Dasatinib__Kinase inhibitor KB-R7943 (mesylate)
Product Name
Dasatinib
Description
Kinase inhibitor
Purity
>98%
CAS No.
302962-49-8
Molecular Formula
C22H26ClN7O2S
Molecular Weight
488.0
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in DMSO (200 mg/ml), ethanol (very poorly ), and water (very poorly )
Source
Synthetic
Appearance
Crystalline solid
SMILES
C1=C(SC(=N1)NC2=CC(=NC(=N2)C)N3CCN(CC3)CCO)C(=O)NC4=C(C=CC=C4Cl)C
InChI
InChI=1S/C22H26ClN7O2S/c1-14-4-3-5-16(23)20(14)28-21(32)17-13-24-22(33-17)27-18-12-19(26-15(2)25-18)30-8-6-29(7-9-30)10-11-31/h3-5,12-13,31H,6-11H2,1-2H3,(H,28,32)(H,24,25,26,27)
InChIKey
ZBNZXTGUTAYRHI-UHFFFAOYSA-N
Safety Phrases
Classification:
Acute Toxicity: Oral, Category 3
Toxic To Reproduction, Category 1B
Target Organ Systemic Toxicity (single exposure), Category 2
Target Organ Systemic Toxicity (repeated exposure), Category 2
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H301: Toxic if swallowed.
H360: May damage fertility or the unborn child.
H370: Causes damage to organs {uterus, testicles,ovaries}.
H373: May cause damage to {uterus, testicles,ovaries} through prolonged or repeated exposure.
Precautionary statements:
P264: Wash {hands} thoroughly after handling.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P260: Do not breathe {dust/fume/gas/mist/vapours/spray}.
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P330: Rinse mouth.
P308+313: IF exposed or concerned: Get medical attentiodvice.
P312: Call a {POISON CENTER/doctor/…} if you feel unwell.
P314: Get medical attentiodvice if you feel unwell.
Cite This Product
Dasatinib (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-440)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19120272
Alternative Names
N-(2-chloro-6-methylphenyl)-2-6-4-(2-hydroxyethyl)piperazin-1-yl-2-methylpyrimidin-4-ylamino-1,3-thiazole-5-carboxamide
Research Areas
Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors
PubChem ID
3062316
Scientific Background
Dasatinib is a dual Src and Abl kinase inhibitor with ∼325 times more potency against BCR-ABL than imatinib. Specifically, it inhibits autophosphorylation and substrate phosphorylation in a concentration-dependent manner.
References
1. Benjamini O., et al. (2014) Am. J. Hematology. 89(3): 282–287.
Kinase inhibitor
Acute Toxicity: Oral, Category 3
Toxic To Reproduction, Category 1B
Target Organ Systemic Toxicity (single exposure), Category 2
Target Organ Systemic Toxicity (repeated exposure), Category 2
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H301: Toxic if swallowed.
H360: May damage fertility or the unborn child.
H370: Causes damage to organs {uterus, testicles,ovaries}.
H373: May cause damage to {uterus, testicles,ovaries} through prolonged or repeated exposure.
Precautionary statements:
P264: Wash {hands} thoroughly after handling.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P260: Do not breathe {dust/fume/gas/mist/vapours/spray}.
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P330: Rinse mouth.
P308+313: IF exposed or concerned: Get medical attentiodvice.
P312: Call a {POISON CENTER/doctor/…} if you feel unwell.
P314: Get medical attentiodvice if you feel unwell.