Anti-Bassoon Antibody__Rabbit Anti-Human Bassoon Polyclonal Bafetinib
Bafilomycin A1
Bafilomycin A1__Autophagy inhibitor Abiraterone (acetate)
Product Name
Bafilomycin A1
Description
Autophagy inhibitor
Purity
>95%
CAS No.
88899-55-2
Molecular Formula
C35H58O9
Molecular Weight
622.84
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in 100% ethanol, methanol or DMSO.
Source
Synthetic
Appearance
White powder to colorless film.
SMILES
C@1(OC@@H(email protected(email protected(O)C1)C)C(C)C)(O)email protected(email protected(O)C@@H(email protected2OC(=O)C(=C/C(=C/email protected(C@@H(O)email protected(CC(=C/C=C/C@@H2OC)
InChI
InChI=1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H
InChIKey
XDHNQDDQEHDUTM-JQWOJBOSSA-N
Safety Phrases
Classification: D2B- Toxic Material Causing Other Toxic Effects Irritant
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H315- Causes skin irritation.
H319- Causes serious eye irritation.
H335- May cause respiratory irritation.
Precautionary statements:
P261- Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264- Wash skin thoroughly after handling.
P271- Use only outdoors or in a well-ventilated area.
P280- Wear protective gloves/ eye protection/ face protection.
P302 + P352- IF ON SKIN: Wash with plenty of soap and water.
P304 + P340- IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for
breathing.
P305 + P351 + P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312- Call a POISON CENTER or doctor/ physician if you feel unwell.
P321- Specific treatment (see supplemental first aid instructions on this label).
P332 + P313- If skin irritation occurs: Get medical advice/ attention.
P337 + P313- If eye irritation persists: Get medical advice/ attention.
P362- Take off contaminated clothing and wash before reuse.
P403 + P233- Store in a well-ventilated place. Keep container tightly closed.
P405- Store locked up.
P501- Dispose of contents/ container to an approved waste disposal plant.
Cite This Product
Bafilomycin A1 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-391)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/1911608
Alternative Names
(3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-(1S,2R,3S)-2-hydroxy-1-methyl-3-(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-ylbutyl-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one
Research Areas
Cancer, Autophagy
PubChem ID
6436223
Scientific Background
Bafilomycin A1 is a macrolide antibiotic derived from Streptomyces griseus. It selectively inhibits vacuolar-type H+-ATPases, allowing it to be used to differentiate between different types of ATPases. Bafilomycin also acts to inhibit autophagy by either blocking autophagosome-lysosome fusion, or by blocking lysosomal degradation.
References
1. Gagliardi S., et al. (1999). Curr Med Chem. 6(12): 1197-212.
2. Fass E., et al. (2006). J Biol Chem. 281(47): 36303-16.
3. Yamamoto A., et al. (1998). Cell Struct Funct. 23(1): 33-42.
Autophagy inhibitor
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H315- Causes skin irritation.
H319- Causes serious eye irritation.
H335- May cause respiratory irritation.
Precautionary statements:
P261- Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264- Wash skin thoroughly after handling.
P271- Use only outdoors or in a well-ventilated area.
P280- Wear protective gloves/ eye protection/ face protection.
P302 + P352- IF ON SKIN: Wash with plenty of soap and water.
P304 + P340- IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for
breathing.
P305 + P351 + P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312- Call a POISON CENTER or doctor/ physician if you feel unwell.
P321- Specific treatment (see supplemental first aid instructions on this label).
P332 + P313- If skin irritation occurs: Get medical advice/ attention.
P337 + P313- If eye irritation persists: Get medical advice/ attention.
P362- Take off contaminated clothing and wash before reuse.
P403 + P233- Store in a well-ventilated place. Keep container tightly closed.
P405- Store locked up.
P501- Dispose of contents/ container to an approved waste disposal plant.
2. Fass E., et al. (2006). J Biol Chem. 281(47): 36303-16.
3. Yamamoto A., et al. (1998). Cell Struct Funct. 23(1): 33-42.
b-AP15 (NSC-687852)
b-AP15 (NSC-687852)__DUB inhibitor Volasertib
Product Name
b-AP15 (NSC-687852)
Description
DUB inhibitor
Purity
>98%
CAS No.
1009817-63-3
Molecular Formula
C22H17N3O6
Molecular Weight
419.4
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in 35 mg/ml DMSO
Source
Synthetic
Appearance
Pale Yellow Solid
SMILES
C=CC(=O)N1C/C(=CC2=CC=C(C=C2)N+(=O)O-)/C(=O)/C(=C/C3=CC=C(C=C3)N+(=O)O-)/C1
InChI
InChI=1S/C22H17N3O6/c1-2-21(26)23-13-17(11-15-3-7-19(8-4-15)24(28)29)22(27)18(14-23)12-16-5-9-20(10-6-16)25(30)31/h2-12H,1,13-14H2/b17-11+,18-12+
InChIKey
GFARQYQBWJLZMW-JYFOCSDGSA-N
Safety Phrases
Classification: Caution – Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
Cite This Product
b-AP15 (NSC-687852) (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-339)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19116074/
Alternative Names
(3E,5E)-1-Acryloyl-3,5-bis(4-nitrobenzylidene)-4-piperidinone
Research Areas
Cancer, Apoptosis
PubChem ID
5351435
Scientific Background
Abrogates the deubiquitinating activity of the 19S regulatory particle by inhibiting ubiquitin C-terminal hydrolase 5 (UCHL5) and ubiquitin-specific peptidase 14 (USP14) resulting in accumulation of polyubiquitin in cells. b-AP15 induces tumor cell apoptosis and tumor progression in four different solid tumor models in vivo.
References
1. D’Arcy P., et al. (2011) Nature Medicine 17: 1636.
DUB inhibitor
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25- Avoid contact with skin and eyes
AZD6244
AZD6244__ERK kinase inhibitor PNU-159682
Product Name
AZD6244
Description
ERK kinase inhibitor
Purity
>98%
CAS No.
606143-52-6
Molecular Formula
C17H15BrClFN4O3
Molecular Weight
457.7
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in DMSO (92 mg/ml at 25 °C), water (<1 mg/ml at 25 °C), and ethanol (<1 mg/ml at 25 °C)
Source
Synthetic
Appearance
White to off-white solid
SMILES
CN1C=NC2=C1C=C(C(=C2F)NC3=C(C=C(C=C3)Br)Cl)C(=O)NOCCO
InChI
InChI=1S/C17H15BrClFN4O3/c1-24-8-21-16-13(24)7-10(17(26)23-27-5-4-25)15(14(16)20)22-12-3-2-9(18)6-11(12)19/h2-3,6-8,22,25H,4-5H2,1H3,(H,23,26)
InChIKey
CYOHGALHFOKKQC-UHFFFAOYSA-N
Safety Phrases
Classification:
Serious Eye Damage/Eye Irritation, Category 1
Skin Sensitization, Category 1
Toxic To Reproduction, Category 2
Target Organ Systemic Toxicity (repeated exposure), Category 2
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H318: Causes serious eye damage.
H317: May cause an allergic skin reaction.
H361: Suspected of damaging fertility or the unborn child.
H373: May cause damage to {Liver, gastrointestinal tract and other major organs} through prolonged or repeated exposure.
Precautionary statements:
P280: Wear {protective gloves/protective clothing/eye protection/face protection}.
P261: Avoid breathing {dust/fume/gas/mist/vapours/spray}.
P272: Contaminated work clothing should not be allowed out of the workplace.
P362+364: Take off contaminated clothing and wash it before reuse.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P260: Do not breathe {dust/fume/gas/mist/vapours/spray}.
P305+351+338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P310: Immediately call a {POISON CENTER/doctor/…}.
P302+352: IF ON SKIN: Wash with plenty of soap and water.
P333+313: If skin irritation or rash occurs, seek medical advice/attention.
P308+313: IF exposed or concerned: Get medical attentiodvice.
P314: Get medical attentiodvice if you feel unwell.
Cite This Product
AZD6244 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-495)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19116064
Alternative Names
Selumetinib, AZD6244, 6-(4-bromo-2-chloroanilino)-7-fluoro-N-(2-hydroxyethoxy)-3-methylbenzimidazole-5-carboxamide
Research Areas
Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors
PubChem ID
10127622
Scientific Background
AZD6244 is a MEK-1 inhibitor. Specifically, AZD6244 inactivates the phosphorylation of ERK1/2.
References
1. Haasbach E., Hartmayer C., & Planz O. (2013) Antiviral Research. 98(2): 319–324.
ERK kinase inhibitor
Serious Eye Damage/Eye Irritation, Category 1
Skin Sensitization, Category 1
Toxic To Reproduction, Category 2
Target Organ Systemic Toxicity (repeated exposure), Category 2
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H318: Causes serious eye damage.
H317: May cause an allergic skin reaction.
H361: Suspected of damaging fertility or the unborn child.
H373: May cause damage to {Liver, gastrointestinal tract and other major organs} through prolonged or repeated exposure.
Precautionary statements:
P280: Wear {protective gloves/protective clothing/eye protection/face protection}.
P261: Avoid breathing {dust/fume/gas/mist/vapours/spray}.
P272: Contaminated work clothing should not be allowed out of the workplace.
P362+364: Take off contaminated clothing and wash it before reuse.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P260: Do not breathe {dust/fume/gas/mist/vapours/spray}.
P305+351+338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P310: Immediately call a {POISON CENTER/doctor/…}.
P302+352: IF ON SKIN: Wash with plenty of soap and water.
P333+313: If skin irritation or rash occurs, seek medical advice/attention.
P308+313: IF exposed or concerned: Get medical attentiodvice.
P314: Get medical attentiodvice if you feel unwell.
Axitinib
Axitinib__VEGFR kinase inhibitor Azeliragon
Product Name
Axitinib
Description
VEGFR kinase inhibitor
Purity
>98% (HPLC)
CAS No.
319460-85-0
Molecular Formula
C22H18N4OS
Molecular Weight
386.5
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble to 25 mM in DMSO
Source
Synthetic
Appearance
White to tan powder
SMILES
CNC(=O)C1=CC=CC=C1SC2=CC3=C(C=C2)C(=NN3)/C=C/C4=CC=CC=N4
InChI
InChI=1S/C22H18N4OS/c1-23-22(27)18-7-2-3-8-21(18)28-16-10-11-17-19(25-26-20(17)14-16)12-9-15-6-4-5-13-24-15/h2-14H,1H3,(H,23,27)(H,25,26)/b12-9+
InChIKey
RITAVMQDGBJQJZ-FMIVXFBMSA-N
Safety Phrases
Classification:
Acute toxicity, Oral (Category 4), H302
Acute aquatic toxicity (Category 1), H400
Chronic aquatic toxicity (Category 1), H410
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H302 Harmful if swallowed.
H410 Very toxic to aquatic life with long lasting effects
Precautionary statements:
P264 Wash skin thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P273 Avoid release to the environment.
P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P330 Rinse mouth.
P391 Collect spillage.
P501 Dispose of contents/ container to an approved waste disposal plant.
Cite This Product
Axitinib (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-494)
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19116032
Alternative Names
AG-013736, N-Methyl-2-((3-((1E)-2-(pyridin-2-yl)ethenyl)-1H-indazol-6-yl)sulfanyl)benzamide, N-Methyl-3(1E)-2-(2-pyridinyl)ethenyl-1H-indazol-6-ylthio-benzamide
Research Areas
Cancer, Apoptosis, Cancer Growth Inhibitors, Cardiovascular System, Cell Signaling, Tyrosine Kinase Inhibitors
PubChem ID
6450551
Scientific Background
Axitinib is a potent and selective tyrosine kinase inhibitor that blocks VEGF receptors 1, 2 and 3. It has also been shown to inhibit PDGFR-β and c-Kit.
References
1. Hu-Lowe D.D., et al. (2008) Clin. Cancer Res: J. Am. Ass. Cancer Res. 14(22): 7272–7283.
VEGFR kinase inhibitor
Acute toxicity, Oral (Category 4), H302
Acute aquatic toxicity (Category 1), H400
Chronic aquatic toxicity (Category 1), H410
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H302 Harmful if swallowed.
H410 Very toxic to aquatic life with long lasting effects
Precautionary statements:
P264 Wash skin thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P273 Avoid release to the environment.
P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P330 Rinse mouth.
P391 Collect spillage.
P501 Dispose of contents/ container to an approved waste disposal plant.
Anti-Aurora C Antibody (pSer193)
Anti-Aurora C Antibody (pSer193)__Rabbit Anti-Human Aurora C (pSer193) Polyclonal (-)-DHMEQ
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 35.591 kDa.
Cite This Product
Rabbit Anti-Human Aurora C (pSer193) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-921)
Certificate of Analysis
A 1:250 dilution of SPC-921 was sufficient for detection of Aurora C (pSer193) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19116009
Alternative Names
AURKC Antibody, AIE2 Antibody, ARK3 Antibody, Aurora/lpl1 related kinase 3 Antibody, Serine threonine protein kinase 13 Antibody, STK13 Antibody, SPGF5 Antibody, IPL-1/STK13/Aurora C Antibody
Cellular Localization
Cytoplasm, Chromosome, Cytoskeleton
Accession Number
NP_001015878
Gene ID
6795
Swiss Prot
Q9UQB9
Scientific Background
Aurora C is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
Anti-Aurora B Antibody (pSer227)
Anti-Aurora B Antibody (pSer227)__Rabbit Anti-Human Aurora B (pSer227) Polyclonal Ruxolitinib
Product Name
Aurora B Antibody (pSer227)
Description
Rabbit Anti-Human Aurora B (pSer227) Polyclonal
Species Reactivity
Human
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser227 of human Aurora B (AA222-228)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa

Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet

Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:

Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:

Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:

Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:

Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:

Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:

Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol

Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:

Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:

Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:

Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 39.28 kDa.
Cite This Product
Rabbit Anti-Human Aurora B (pSer227) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-920)
Certificate of Analysis
A 1:250 dilution of SPC-920 was sufficient for detection of Aurora B (pSer227) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19115993
Alternative Names
AIK2 Antibody, ARK2 Antibody, Aurora related Kinase 2 Antibody, IPL1 Antibody, STK1 Antibody, Aurora 1 Antibody
Cellular Localization
Cytoplasm, Cytoskeleton, Nucleus
Accession Number
NP_004208
Gene ID
9212
Swiss Prot
Q96GD4
Scientific Background
Aurora B is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
Rabbit Anti-Human Aurora B (pSer227) Polyclonal
WB
,
AM
APC (Allophycocyanin) | ||
Overview:
|
![]() |
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
![]() |
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
ATTO 488 | ||
Overview: | ![]() |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
ATTO 565 | ||
Overview: | ![]() |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
ATTO 594 | ||
Overview: | ![]() |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
ATTO 633 | ||
Overview: | ![]() |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
ATTO 655 | ||
Overview: | ![]() |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
ATTO 680 | ||
Overview: | ![]() |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
ATTO 700 | ||
Overview:
|
![]() |
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
FITC (Fluorescein) | ||
Overview: | ![]() |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
PE/ATTO 594 | ||
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
Overview: | ![]() |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
PerCP | ||
Overview: | ![]() |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
R-PE (R-Phycoerythrin) | ||
Overview: | ![]() |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Anti-Aurora B Antibody
Anti-Aurora B Antibody__Rabbit Anti-Human Aurora B Polyclonal NVP-TNKS656
Product Name
Aurora B Antibody
Description
Rabbit Anti-Human Aurora B Polyclonal
Species Reactivity
Human, Mouse
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
Synthetic peptide of human Aurora B (AA66-80)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa

Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet

Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:

Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:

Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:

Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:

Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:

Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:

Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol

Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:

Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:

Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:

Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 39.28 kDa.
Cite This Product
Rabbit Anti-Human Aurora B Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1108)
Certificate of Analysis
A 1:250 dilution of SPC-1108 was sufficient for detection of Aurora B in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19115957
Alternative Names
AIK2 Antibody, ARK2 Antibody, Aurora related Kinase 2 Antibody, IPL1 Antibody, STK1 Antibody, Aurora 1 Antibody
Cellular Localization
Cytoplasm, Cytoskeleton, Nucleus
Accession Number
NP_004208
Gene ID
9212
Swiss Prot
Q96GD4
Scientific Background
Aurora B is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
Rabbit Anti-Human Aurora B Polyclonal
WB
,
AM
APC (Allophycocyanin) | ||
Overview:
|
![]() |
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
![]() |
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
ATTO 488 | ||
Overview: | ![]() |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
ATTO 565 | ||
Overview: | ![]() |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
ATTO 594 | ||
Overview: | ![]() |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
ATTO 633 | ||
Overview: | ![]() |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
ATTO 655 | ||
Overview: | ![]() |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
ATTO 680 | ||
Overview: | ![]() |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
ATTO 700 | ||
Overview:
|
![]() |
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
FITC (Fluorescein) | ||
Overview: | ![]() |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
PE/ATTO 594 | ||
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
Overview: | ![]() |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
PerCP | ||
Overview: | ![]() |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
R-PE (R-Phycoerythrin) | ||
Overview: | ![]() |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Anti-Aurora B Antibody
Anti-Aurora B Antibody__Rabbit Anti-Human Aurora B Polyclonal PCI-32765
Product Name
Aurora B Antibody
Description
Rabbit Anti-Human Aurora B Polyclonal
Species Reactivity
Human, Mouse
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
Synthetic peptide of human Aurora B (AA2-16)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa

Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet

Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:

Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:

Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:

Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:

Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:

Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:

Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol

Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:

Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:

Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:

Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 39.28 kDa.
Cite This Product
Rabbit Anti-Human Aurora B Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1107)
Certificate of Analysis
A 1:250 dilution of SPC-1107 was sufficient for detection of Aurora B in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19115949
Alternative Names
AIK2 Antibody, ARK2 Antibody, Aurora related Kinase 2 Antibody, IPL1 Antibody, STK1 Antibody, Aurora 1 Antibody
Cellular Localization
Cytoplasm, Cytoskeleton, Nucleus
Accession Number
NP_004208
Gene ID
9212
Swiss Prot
Q96GD4
Scientific Background
Aurora B is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
Rabbit Anti-Human Aurora B Polyclonal
WB
,
AM
APC (Allophycocyanin) | ||
Overview:
|
![]() |
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
![]() |
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
ATTO 488 | ||
Overview: | ![]() |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
ATTO 565 | ||
Overview: | ![]() |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
ATTO 594 | ||
Overview: | ![]() |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
ATTO 633 | ||
Overview: | ![]() |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
ATTO 655 | ||
Overview: | ![]() |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
ATTO 680 | ||
Overview: | ![]() |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
ATTO 700 | ||
Overview:
|
![]() |
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
FITC (Fluorescein) | ||
Overview: | ![]() |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
PE/ATTO 594 | ||
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
Overview: | ![]() |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
PerCP | ||
Overview: | ![]() |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
R-PE (R-Phycoerythrin) | ||
Overview: | ![]() |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Anti-Aurora A Antibody (pThr287 + pThr288)
Anti-Aurora A Antibody (pThr287 + pThr288)__Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal Calicheamicin
Product Name
Aurora A Antibody (pThr287 + pThr288)
Description
Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal
Species Reactivity
Human
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr287 and Thr288 of human Aurora A (AA285-291)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa

Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet

Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:

Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:

Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:

Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:

Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:

Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:

Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol

Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:

Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:

Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:

Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects ~48kDa.
Cite This Product
Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-919)
Certificate of Analysis
A 1:250 dilution of SPC-919 was sufficient for detection of Aurora A (pThr287 + pThr288) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID::http://www.ncbi.nlm.nih.gov/pubmed/19115845
Alternative Names
Aurora 2 Antibody, AIK Antibody, ARK1 Antibody, BTAK Antibody
Cellular Localization
Cytoplasm, Cytoskeleton
Accession Number
NP_003591
Gene ID
6790
Swiss Prot
O14965
Scientific Background
Aurora A is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal
WB
,
AM
APC (Allophycocyanin) | ||
Overview:
|
![]() |
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
![]() |
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
ATTO 488 | ||
Overview: | ![]() |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
ATTO 565 | ||
Overview: | ![]() |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
ATTO 594 | ||
Overview: | ![]() |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
ATTO 633 | ||
Overview: | ![]() |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
ATTO 655 | ||
Overview: | ![]() |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
ATTO 680 | ||
Overview: | ![]() |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
ATTO 700 | ||
Overview:
|
![]() |
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
FITC (Fluorescein) | ||
Overview: | ![]() |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
PE/ATTO 594 | ||
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
Overview: | ![]() |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
PerCP | ||
Overview: | ![]() |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
R-PE (R-Phycoerythrin) | ||
Overview: | ![]() |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA