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2-APB

2-APB__TRP blocker PF-3084014

Product Name 2-APB

Description
TRP blocker

Purity >98%

CAS No. 524-95-8

Molecular Formula C14H16BNO

Molecular Weight 225.1

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble to 100 mM in DMSO and to 10 mM in ethanol

Source Synthetic

Appearance White solid

SMILES B(C1=CC=CC=C1)(C2=CC=CC=C2)OCCN

InChI InChI=1S/C14H16BNO/c16-11-12-17-15(13-7-3-1-4-8-13)14-9-5-2-6-10-14/h1-10H,11-12,16H2

InChIKey BLZVCIGGICSWIG-UHFFFAOYSA-N

Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Hazard Phrases:
H302-H315-H317-H318-H335
Precautionary Phrases:
P261-P280-P305 + P351 + P338

Cite This Product 2-APB (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-316)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19110265

Alternative Names 2-Aminoethoxydiphenylborane

Research Areas Ion Channels, Neuroscience, Transient Receptor Potential Channels

PubChem ID 1598

Scientific Background 2-APB is a chemical that acts to inhibit IP3 receptors (1) and TRP channels (2). It is used to manipulate intracellular release of calcium ions and modify TRP channel activity. There is also evidence that 2-APB acts directly to inhibit gap junctions made of connexin26 or connexin32 (3).

References 1. Diver J.M., Sage S.O., Rosado J.A. (2001) Cell Calcium. 30(5): 323-329
2. Xu S.Z., et al. (2005) Br J Pharamcol. 145(4): 320-328.
3. Tao L., and Harris A.L. (2007) Mol Pharmacol. 71(2): 570-579.

17-GMB-APA-GA

17-GMB-APA-GA__Hsp90 inhibitor ARS-853

Product Name 17-GMB-APA-GA

Description
Hsp90 inhibitor

Purity >98%

Molecular Formula C39H53N5O11

Molecular Weight 767.9

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO (>25 mg/ml) and ethanol (10 mg/ml)

Source Synthetic

Appearance Purple Solid

SMILES O=C/1C2=C(/C(=O)C=C1NC(=O)C(=CC=C[[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/* ]]> /](OC)[C@@H](OC(=O)N)C(=C/[[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/ ]]> /](C)[C@@H](O)[C@@H](OC)C[[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/ ]]> */](C)C2)/C)C)NCCCNC(=O)CCCN3C(=O)C=C/C3=O

InChI InChI=1S/C39H53N5O11/c1-22-18-26-34(42-16-9-15-41-31(46)12-8-17-44-32(47)13-14-33(44)48)28(45)21-27(36(26)50)43-38(51)23(2)10-7-11-29(53-5)37(55-39(40)52)25(4)20-24(3)35(49)30(19-22)54-6/h7,10-11,13-

InChIKey OPWAYYOLUIAKCJ-DAJJPBRBSA-N

Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection

Cite This Product 17-GMB-APA-GA (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-115)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19110213

Alternative Names [(3R,5S,6R,7S,10S,11S)-21-[3-[4-(2,5-dioxopyrrol-1-yl)butanoylamino]propylamino]-6-hydroxy-5,11-dimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

Research Areas Cancer, Heat Shock

PubChem ID 57370016

Scientific Background An analog of geldanamycin containing a maleimido moiety suitable for preparation of geldanamycin immunoconjugates (1-3). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References 1. Mandler R., et al. (2004) Cancer Res. 64: 1460.
2. Mandler R., et al. (2002) Bioconj. Chem. 13: 786.
3. Mandler R., et al. (2000) J. Natl. Cancer Inst. 92: 1573.

17-DMAG

17-DMAG__Hsp90 inhibitor SKF-96365 (hydrochloride)

Product Name 17-DMAG

Description
Hsp90 inhibitor

Purity >98% (TLC); NMR (Conforms)

CAS No. 467214-20-6

Molecular Formula C32H48N4O8

Molecular Weight 616.8

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO (30 mg/ml) and ethanol (10 mg/ml)

Source Synthetic

Appearance Purple Solid

SMILES C[[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/* ]]> /]1C[C@@H]([C@@H]([[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/ ]]> /](/C=C(/[C@@H]([[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/ ]]> */](/C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)NCCN(C)C)/C)OC)OC(=O)N)C)C)O)OC

InChI InChI=1S/C32H48N4O8/c1-18-14-22-27(34-12-13-36(5)6)24(37)17-23(29(22)39)35-31(40)19(2)10-9-11-25(42-7)30(44-32(33)41)21(4)16-20(3)28(38)26(15-18)43-8/h9-11,16-18,20,25-26,28,30,34,38H,12-15H2,1-8H3,(

InChIKey KUFRQPKVAWMTJO-LMZWQJSESA-N

Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection

Cite This Product 17-DMAG (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-114)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19110212

Alternative Names Alvespimycin, 17DMAG, 17-(dimethylaminoethylamino)-17-demethoxy-geldanamycin, 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin, 17-desmethoxy-17-n,n-dimethylaminoethylamino-geldanamycin, 17-dimethylaminoethylamino-17-demethoxy-geldanamycin, 17-DMAG, 17-Dimethylaminoethylamino, 17-Demethoxygeldanamycin, [(3R,5S,6R,7S,8E,10S,11S,12Z,14E)-21-[2-(dimethylamino)ethylamino]-6-hydroxy-5,11-dimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

Research Areas Cancer, Heat Shock

PubChem ID 5288674

Scientific Background 17-DMAG is a water soluble & cell-permeable analog of Geldanamycin and 17-AAG (1). It binds to the APTase site of human Hsp90a with high affinity, has cytotoxic activity against many cancer cell lines (2), and acts as angiogenesis inhibitor (3). This Hsp90 inhibitor shows promise in preclinical models. 17-DMAG has excellent bioavailability, is widely distributed to tissues, and is quantitatively metabolized much less than is 17-AAG.

References 1. Bull E.E., et al.(2004) Clin. Cancer Res. 10: 8077.
2. Gossett D.R. et al.(2005) Gynecol. Oncol. 96: 381.
3. Kaur G. et al.(2004) Clin. Cancer Res. 10: 4813.

17-AAG

17-AAG__Hsp90 inhibitor PKC412

Product Name 17-AAG

Description
Hsp90 inhibitor

Purity >98% (TLC); NMR (Conforms)

CAS No. 75747-14-7

Molecular Formula C31H43N3O8

Molecular Weight 585.7

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO (>50 mg/ml) or ethanol (5 mg/ml)

Source Synthetic

Appearance Red to dark red powder

SMILES C[[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/* ]]> /]1C[C@@H]([C@@H]([[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/ ]]> /](/C=C(/[C@@H]([[email protected]function(t,e,r,n,c,a,p){try{t=document.currentScript||function(){for(t=document.getElementsByTagName(_script_),e=t.length;e–;)if(t[e].getAttribute(_data-cfhash_))return t[e]}();if(t&&(c=t.previousSibling)){p=t.parentNode;if(a=c.getAttribute(_data-cfemail_)){for(e=__,r=_0x_+a.substr(0,2)|0,n=2;a.length-n;n+=2)e+=_%_+(_0_+(_0x_+a.substr(n,2)^r).toString(16)).slice(-2);p.replaceChild(document.createTextNode(decodeURIComponent(e)),c)}p.removeChild(t)}}catch(u){}}()/ ]]> */](/C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)NCC=C)/C)OC)OC(=O)N)C)C)O)OC

InChI InChI=1S/C31H43N3O8/c1-8-12-33-26-21-13-17(2)14-25(41-7)27(36)19(4)15-20(5)29(42-31(32)39)24(40-6)11-9-10-18(3)30

InChIKey AYUNIORJHRXIBJ-TXHRRWQRSA-N

Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection

Cite This Product 17-AAG (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-100)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19110202

Alternative Names 17-(Allylamino)-17-demethoxygeldanamycin, 17-(Allylamino)geldanamycin, 17-Demethoxy-17-allylamino geldanamycin, 17-AAG, CP 127374, Geldanamycin,17-demethoxy-17-(2-propenylamino)-, NSC 330507, Tanespimycin

Research Areas Cancer, Heat Shock

PubChem ID 6505803

Scientific Background Glendanamycin (GA), a benzoquinone ansamycin antibiotic, interferes with the action of Hsp90 leading to degradation of Hsp90 client proteins. GA itself however has undesirable properties such as poor aqueous solubility and liver toxicity; therefore, numerous analogs have been synthesized, such as 17-AAG(1). 17-AAG is an HSP-90 inhibitor that displays a 100-fold higher affinity for HSP-90 derived from tumor cells compared to HSP-90 from normal cells(2). 17-AAG inhibits Akt activation and expression in tumors and synergizes with a number of antitumor agents such as taxol(3), cisplatin(4) and UCN-01 (400 nM 17-AAG, U937 cells)(5). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References 1. Neckers L. (2002) Trends Mol Med. 84: S55-61.
2. Kamal A., et al.(2003) Nature. 425: 407.
3. Solit D.B., et al.(2003) Cancer Res. 63: 2139.
4. Vasilevskaya I.A., et al. (2003) Mol.Pharmacol. 65: 235.
5. Jia W., et al.(2003) Blood. 102: 1824.

AAL 993

AAL 993__VEGFR inhibitor ABT-199

Product Name AAL 993

Description
VEGFR inhibitor

Purity >98% (TLC)

CAS No. 269390-77-4

Molecular Formula C20H16F3N3O

Molecular Weight 371.4

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO (>25 mg/ml) or ethanol (15 mg/ml)

Source Synthetic

Appearance Pale yellow solid

SMILES C1=CC=C(C(=C1)C(=O)NC2=CC=CC(=C2)C(F)(F)F)NCC3=CC=NC=C3

InChI InChI=1S/C20H16F3N3O/c21-20(22,23)15-4-3-5-16(12-15)26-19(27)17-6-1-2-7-18(17)25-13-14-8-10-24-11-9-14/h1-12,25H,13H2,(H,26,27)

InChIKey BLAFVGLBBOPRLP-UHFFFAOYSA-N

Safety Phrases Classification:
Not a hazardous substance or mixture.

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.

Cite This Product AAL 993 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-424)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19111409

Alternative Names 2-[(4-Pyridyl)methyl]amino-N-[3-(trifluoromethyl)phenyl]benzamide

Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cardiovascular System, Cell Signaling, Growth Factor Receptor Inhibitors

PubChem ID 6398883

Scientific Background AAL-993 is a very potent inhibitor of VEGFR-1, VEGFR-2 and VEGFR-3, as well as PDGFR-β, c-Kit and CSF-1R at higher concentrations.

References 1. Manley P.W., et al. (2004) Biochimica Biophysica Acta. 1697(1-2): 17–27.
2. Ban H.S., Uno M., & Nakamura H. (2010) Cancer Letters. 296(1): 17–26.

Anti-AAK1 Antibody (pSer637)

Anti-AAK1 Antibody (pSer637)__Rabbit Anti-Human AAK1 (pSer637) Polyclonal PAK4-IN-1

Product Name AAK1 Antibody (pSer637)

Description
Rabbit Anti-Human AAK1 (pSer637) Polyclonal

Species Reactivity Human

Applications ,
WB
,
AM

Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen A phospho-specific peptide corresponding to residues surrounding Ser637 of human AAK1 (AA634-640)

Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)

Overview:

High quantum yield

Large phycobiliprotein

6 chromophores per molecule

Isolated from red algae

Molecular Weight: 105 kDa

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

ATTO 390

Overview:

High fluorescence yield

Large Stokes-shift (89 nm)

Good photostability

Moderately hydrophilic

Good solubility in polar solvents

Coumarin derivate, uncharged

Low molar mass: 343.42 g/mol

ATTO 390 Datasheet
Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

ATTO 488

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 804 g/mol

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 565

Overview:

High fluorescence yield

High thermal and photostability

Good solubility in polar solvents

Excellent solubility in water

Very little aggregation

Rhodamine dye derivative

Molar Mass: 611 g/mol

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 594

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 1137 g/mol

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 633

Overview:

High fluorescence yield

High thermal and photostability

Moderately hydrophilic

Good solubility in polar solvents

Stable at pH 4 – 11

Cationic dye, perchlorate salt

Molar Mass: 652.2 g/mol

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 655

Overview:

High fluorescence yield

High thermal and photostability

Excellent ozone resistance

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 634 g/mol

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 680

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 631 g/mol

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 700

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 575 g/mol

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield

High rate of photobleaching

Good solubility in water

Broad emission spectrum

pH dependent spectra

Molecular formula: C₂₀H₁₂O₅

Molar mass: 332.3 g/mol

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex

Small phycobiliprotein

Isolated from red algae

Large stokes shift (195 nm)

Molecular Weight: 35 kDa

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum

High quantum yield

Photostable

Member of the phycobiliprotein family

Isolated from red algae

Excellent solubility in water

Molecular Weight: 250 kDa

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols

Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation

Catalyzes the conversion of:

Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products

Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products

Molecular weight: 140 kDa

Applications: Western blot, immunohistochemistry, and ELISA

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target

Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors

Catalyzes the conversion of:

Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products

Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)

Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products

High turnover rate enables rapid generation of a strong signal

44 kDa glycoprotein

Extinction coefficient: 100 (403 nm)

Applications: Western blot, immunohistochemistry, and ELISA

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity

Molar mass: 244.31 g/mol

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity

Molecular weight: 53 kDa

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Storage Temperature -20ºC

Shipping Temperature Blue Ice or 4ºC

Purification Peptide Affinity Purified

Clonality Polyclonal

Specificity Detects 93.578 kDa.

Cite This Product Rabbit Anti-Human AAK1 (pSer637) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-901)

Certificate of Analysis A 1:250 dilution of SPC-901 was sufficient for detection of AAK1 (pSer637) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19111386

Alternative Names AAK1_HUMAN Antibody, Adaptor associated kinase 1 Antibody, AP2 associated kinase 1 AAntibody, DKFZp686F03202 Antibody, DKFZp686K16132 Antibody

Cellular Localization Membrane

Accession Number NP_002813

Gene ID 22848

Swiss Prot Q2M2I8

Scientific Background AAK1 is a serine/threonine kinase that is thought to coordinate the recruitment of AP-2 to receptors containing tyrosine-based internalization motifs by phosphorylating the µ2 subunit. There is strong evidence that this protein is the endogenous mu 2 kinase and plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

A83 01

A83 01__ALK kinase inhibitor Arginase inhibitor 1

Product Name A83 01

Description
ALK kinase inhibitor

Purity >98% (HPLC)

CAS No. 909910-43-6

Molecular Formula C25H19N5S

Molecular Weight 421.5

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble to 50 mM in DMSO

Source Synthetic

Appearance White to beige powder

SMILES CC1=NC(=CC=C1)C2=NN(C=C2C3=CC=NC4=CC=CC=C34)C(=S)NC5=CC=CC=C5

InChI InChI=1S/C25H19N5S/c1-17-8-7-13-23(27-17)24-21(19-14-15-26-22-12-6-5-11-20(19)22)16-30(29-24)25(31)28-18-9-3-2-4-10-18/h2-16H,1H3,(H,28,31)

InChIKey HIJMSZGHKQPPJS-UHFFFAOYSA-N

Safety Phrases Classification:
Acute toxicity, Oral (Category 4) Skin irritation (Category 2) Eye irritation (Category 2A) Specific target organ toxicity-single exposure (Category 3), Respiratory system

Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.

Hazard statements:
H302 Harmful if swallowed.
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H335 May cause respiratory irritation..

Precautionary statements:
P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Cite This Product A83 01 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-423)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19111362

Alternative Names 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide, A-83-01, A83-01

Research Areas Cancer, Apoptosis, Cancer Growth Inhibitors, Cell Signaling, Tyrosine Kinase Inhibitors

PubChem ID 16218924

Scientific Background A83-01 is a selective inhibitor of TGF-β type I receptor ALK5 kinase. It blocks phosphorylation of Smad2 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. A 83-01 also inhibits the transcriptional activity induced by TGFβ type I receptor ALK-5, activin type IB receptor ALK-4 and nodal type I receptor ALK-7.

References 1. Li W., et al. (2009) Stem Cell 4(1): 16–19.
2. Cherukuri P., et al. (2012). PloS One. 7(11): e50066.

Anti-A6 Antibody (pTyr309)

Anti-A6 Antibody (pTyr309)__Rabbit Anti-Human A6 (pTyr309) Polyclonal LMI070

Product Name A6 Antibody (pTyr309)

Description
Rabbit Anti-Human A6 (pTyr309) Polyclonal

Species Reactivity Human

Applications ,
WB
,
AM

Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen A phospho-specific peptide corresponding to residues surrounding Tyr309 of human A6 (AA 306-312)

Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

APC (Allophycocyanin)

Overview:

High quantum yield

Large phycobiliprotein

6 chromophores per molecule

Isolated from red algae

Molecular Weight: 105 kDa

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

ATTO 390

Overview:

High fluorescence yield

Large Stokes-shift (89 nm)

Good photostability

Moderately hydrophilic

Good solubility in polar solvents

Coumarin derivate, uncharged

Low molar mass: 343.42 g/mol

ATTO 390 Datasheet
Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

ATTO 488

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 804 g/mol

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 565

Overview:

High fluorescence yield

High thermal and photostability

Good solubility in polar solvents

Excellent solubility in water

Very little aggregation

Rhodamine dye derivative

Molar Mass: 611 g/mol

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 594

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

New dye with net charge of -1

Molar Mass: 1137 g/mol

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 633

Overview:

High fluorescence yield

High thermal and photostability

Moderately hydrophilic

Good solubility in polar solvents

Stable at pH 4 – 11

Cationic dye, perchlorate salt

Molar Mass: 652.2 g/mol

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 655

Overview:

High fluorescence yield

High thermal and photostability

Excellent ozone resistance

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 634 g/mol

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 680

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 631 g/mol

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 700

Overview:

High fluorescence yield

Excellent thermal and photostability

Quenched by electron donors

Very hydrophilic

Good solubility in polar solvents

Zwitterionic dye

Molar Mass: 575 g/mol

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield

High rate of photobleaching

Good solubility in water

Broad emission spectrum

pH dependent spectra

Molecular formula: C₂₀H₁₂O₅

Molar mass: 332.3 g/mol

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield

High photostability

Very hydrophilic

Excellent solubility in water

Very little aggregation

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex

Small phycobiliprotein

Isolated from red algae

Large stokes shift (195 nm)

Molecular Weight: 35 kDa

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum

High quantum yield

Photostable

Member of the phycobiliprotein family

Isolated from red algae

Excellent solubility in water

Molecular Weight: 250 kDa

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols

Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation

Catalyzes the conversion of:

Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products

Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products

Molecular weight: 140 kDa

Applications: Western blot, immunohistochemistry, and ELISA

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target

Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors

Catalyzes the conversion of:

Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products

Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)

Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products

High turnover rate enables rapid generation of a strong signal

44 kDa glycoprotein

Extinction coefficient: 100 (403 nm)

Applications: Western blot, immunohistochemistry, and ELISA

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity

Molar mass: 244.31 g/mol

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity

Molecular weight: 53 kDa

Formula: C₁₀H₁₆N₂O₃S

Applications: Western blot, immunohistochemistry, and ELISA

Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Storage Temperature -20ºC

Shipping Temperature Blue Ice or 4ºC

Purification Peptide Affinity Purified

Clonality Polyclonal

Specificity Detects ~40kDa.

Cite This Product Rabbit Anti-Human A6 (pTyr309) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-900)

Certificate of Analysis A 1:250 dilution of SPC-900 was sufficient for detection of A6 (pTyr309) in 10 µg of human glioblastoma cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19111334

Alternative Names A6 antibody, A6 protein tyrosine kinase antibody, Protein A6 antibody, Protein tyrosine kinase 9 antibody, PTK9 antibody, PTK9 protein tyrosine kinase 9 antibody, TWF 1 antibody, twf1 antibody, TWF1_HUMAN antibody, Twinfilin 1 antibody, Twinfilin actin binding protein, homolog 1 antibody, Twinfilin antibody, Twinfilin-1 antibody

Cellular Localization Cytoplasm, Cytoskeleton

Accession Number NP_002737

Gene ID 5756

Swiss Prot Q12792

Scientific Background A6 appears to be an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, probably by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. It seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

A-967079

A-967079__TRPA1 blocker GSK126

Product Name A-967079

Description
TRPA1 blocker

Purity >98%

CAS No. 1170613-55-4

Molecular Formula C12H14FNO

Molecular Weight 207.3

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble in DMSO (25 mg/ml)

Source Synthetic

Appearance White solid

SMILES CC/C(=NO)/C(=C/C1=CC=C(C=C1)F)/C

InChI InChI=1S/C12H14FNO/c1-3-12(14-15)9(2)8-10-4-6-11(13)7-5-10/h4-8,15H,3H2,1-2H3/b9-8+,14-12+

InChIKey HKROEBDHHKMNBZ-CHBKHGQFSA-N

Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Hazard Phrases:
H302-H315-H317-H318-H335
Precautionary Phrases:
P261-P280-P305 + P351 + P338

Cite This Product A-967079 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-301)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19111245

Alternative Names (1E,3E)-1-(4-Fluorophenyl)-N-hydroxy-2-methyl-1-penten-3-imine

Research Areas Ion Channels, Neuroscience, Transient Receptor Potential Channels

PubChem ID 42641861

Scientific Background A-967079 is a TRPA1 antagonist (1) and cell permeable. It has been found to be a potent blocker of TRPA1 channels. It is an oxime analogue and resembles AP18 from a structural perspective, although it has a methyl addition and a chlorine–ﬂuorine substitution. However, compared with AP18 and HC-030031, A-967079 is signiﬁcantly more potent (100-fold on human and 24-fold on rat TRPA1) and has a much improved pharmacokinetic proﬁle. A-967079 is also more selective, with >1000-fold selectivity over other TRP channels and >150-fold selectivity over 75 other tested proteins (2).

References 1. McGaraughty S., et al. (2010) Mol Pain. 6: 14. 2. Chen et al. (2011) PAIN. 152: 1165–1172.

A-803467

A-803467__NaV1.8 blocker RG7388

Product Name A-803467

Description
NaV1.8 blocker

Purity >98%

CAS No. 944261-79-4

Molecular Formula C19H16ClNO4

Molecular Weight 357.79

Storage Temperature -20ºC

Shipping Temperature Shipped Ambient

Product Type Inhibitor

Solubility Soluble to 100 mM in DMSO and to 25 mM in ethanol

Source Synthetic

Appearance Beige Solid

SMILES COC1=CC(=CC(=C1)NC(=O)C2=CC=C(O2)C3=CC=C(C=C3)Cl)OC

InChI InChI=1S/C19H16ClNO4/c1-23-15-9-14(10-16(11-15)24-2)21-19(22)18-8-7-17(25-18)12-3-5-13(20)6-4-12/h3-11H,1-2H3,(H,21,22)

InChIKey VHKBTPQDHDSBSP-UHFFFAOYSA-N

Safety Phrases Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Hazard Phrases: H3012

Cite This Product A-803467 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-304)

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19111223

Alternative Names 5-(4-Chlorophenyl)-N-(3,5-dimethoxyphenyl)-2-furamide

Research Areas Ion Channels, Neuroscience, Sodium Channels

PubChem ID 16038374

Scientific Background A selective blocker of Nav1.8 channels. A-803467 potently reduced thermal hyperalgesia in inflammatory models of pain and mechanical allodynia in neuropathic models of pain, but was relatively ineffective in a skin-incision model of postoperative pain, a chemotherapy induced pain, and in visceral pain (1).

References 1. Rush A.M., Cummins T.R. (2007) Mol Interv. 7(4): 192-195.