Gnificantly diminished in the CBZ93 rats. On the other hand, the
Gnificantly diminished in the CBZ93 rats. On the other hand, the absolute and relative weights of the ventral prostate as well as the prostatic volume and length of rats from PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25112874 the other CBZ-treated groups (CBZ43 and CBZ63) did not show significant statistical differences in comparison to their control groups (Table 1). Reductions of the volume of the ventral prostate glandular lumen, and of glandular epithelium and fibromuscular stroma were also observed in the CBZ93 group comparatively to their respective control rats (Table 2). buy Luteolin 7-O-��-D-glucoside Moreover, a decrease in the surface density (Sv) and surface (S) of the epithelium of the glandular tubules were also noted in the CBZ93 group (Figures 1 and 2). The volume and volume density of the ventral prostate lumen, epithelium of the glandular tubules and stroma compartments of the CBZ63 and CBZ43 did not show significant alterations in comparison with the control rats of the same age (data not shown).Histological analysis and numerical densities of mast cells and macrophagesAt least five histological sections were analyzed per group and AR positive nuclei were randomly selected by section (100 nuclei/group). The images of the selected nuclei were cut in a regular and constant rectangular form (always in central part of the nucleus, in view of the homogeneity of nuclear staining/reactivity for AR) and those “rectangles” were submitted to optical densitometry analysis by Scion Image for Windows Software ?. The integrated optical density (IOD) methodological proceedings were based on physical principles of quantification performed [39].Hormonal levelsTestosterone and estradiol plasmatic levels were determined by radioimmunoassay method using Coat-ACount Total Testosterone kit and Coat-A-Count Total Estradiol kit (Diagnostic Products Corp., LA, CA, USA). The sensitivity of all Kits utilized was 0.01 ng/mL.Statistical analysisData obtained from the treated and control groups were compared using two-way ANOVA; post hoc “t” parametric test (Student test) or the non-parametric MannWhitney tests were utilized. The differences were considered statistically significant when p 0.05.ResultsMorphometric and stereological evaluationTreatment with CBZ caused a significant decrease in the ventral prostate absolute weight of the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27663262 CBZ93 rats inThe sections of the rat ventral prostate of all control and CBZ-treated groups showed acini surrounded by a fibromuscular stroma, which frequently showed secretion within the lumen. In all groups, the mucosa of the ventral prostate was typically folded showing tall columnar secretory epithelial cells (Figures 3A, E). In the fibromuscular stroma, layers of smooth muscle fibers were observed involving the acini; outer and extensive connective tissue surrounded these structures (Figures 3A-F). Mast cells were found in the stroma of the control (Figures 3A, C, E) and CBZ-treated animals (Figures 3B, D, F and 4A-C). However, cytoplasm of mast cells showed accentuated granules in CBZ43 (Figure 3B) and CBZ63 groups (Figures 3D and 4B, C). Frequently, the granules appeared to be releasing into the connective tissue, suggesting an activation of the mast cells (Figure 3D and 4C). Nevertheless, the CBZ93 rats (Figure 3F) showed mast cells with similar morphological characteristics when compared to those of the C93 control group (Figure 3E). Moreover, the numerical density of mast cells was significantly higher in CBZ63 and CBZ93 groups in comparison to corresponding controls groups (Figure 5).