Help. We thank K. Waki for constructing the pCMVdeltaR8.2/PRclone and.
Help. We thank K. Waki for constructing the pCMVdeltaR8.2/PRclone and. J. Burns for providing the VSV-G expression vector and R. Montelaro for anti-EIAV serum. HIV-Ig was obtained from the NIH AIDS Research and Reference Reagent Program. Author BAY1217389 manufacturer details Laboratory of Molecular Modeling Drug Design; Lindsley F. Kimball Research Institute of the New York Blood Center, 310 E 67th Street, New York, NY 10065, USA. 2New York Structural Biology Center, 89 Convent Avenue, New York, NY, 10027. USA. 3Virus-Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute-Frederick, Frederick, MD 21702, USA. 4School of Chemistry, Joseph Black Building, University of Glasgow,Cytotoxicity of peptides in MT-2 cells and PBMC was measured by the XTT [(sodium 3′-(1-(phenylamino)-Zhang et al. Retrovirology 2011, 8:28 http://www.retrovirology.com/content/8/1/Page 16 ofGlasgow G12 8QQ, U.K. 5Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, USA. Authors’ contributions HZ carried out in vitro and cell-based assays, cell penetration study, circular dichroism, electron microscopy, isothermal titration calorimetry and sedimentation equilibrium analyses and analyzed data; FC carried out the antiviral assays and analyzed data; XZ helped in preparing and purifying proteins; SB performed all NMR related studies, analyzed the data and written part of the manuscript; AAW performed the study on HIV and EIAV assembly and release, prepared the corresponding figures; AC, analyzed the ITC data and written part of the manuscript; DC, analyzed the NMR data and written part of the manuscript; EOF analyzed the data and written and edited the manuscript, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26104484 AKD conceived the study, designed all stapled and other peptides, planned experiments, analyzed data, written and edited the manuscript. All authors read and PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28724915 approved the final manuscript. Competing interests The authors declare that they have no competing interest Received: 20 September 2010 Accepted: 3 May 2011 Published: 3 May 2011 References 1. Briggs JA, Wilk T, Welker R, Krausslich HG, Fuller SD: Structural organization of authentic, mature HIV-1 virions and cores. EMBO J 2003, 22:1707-1715. 2. Briggs JA, Grunewald K, Glass B, Forster F, Krausslich HG, Fuller SD: The mechanism of HIV-1 core assembly: insights from three-dimensional reconstructions of authentic virions. Structure 2006, 14:15-20. 3. Li S, Hill CP, Sundquist WI, Finch JT: Image reconstructions of helical assemblies of the HIV-1 CA protein. Nature 2000, 407:409-413. 4. Ganser BK, Li S, Klishko VY, Finch JT, Sundquist WI: Assembly and analysis of conical models for the HIV-1 core. Science 1999, 283:80-83. 5. Ganser-Pornillos BK, Cheng A, Yeager M: Structure of full-length HIV-1 CA: a model for the mature capsid lattice. Cell 2007, 131:70-79. 6. Ganser-Pornillos BK, Yeager M, Sundquist WI: The structural biology of HIV assembly. Curr Opin Struct Biol 2008, 18:203-217. 7. Pornillos O, Ganser-Pornillos BK, Kelly BN, Hua Y, Whitby FG, Stout CD, Sundquist WI, Hill CP, Yeager M: X-ray structures of the hexameric building block of the HIV capsid. Cell 2009, 137:1282-1292. 8. Ako-Adjei D, Johnson MC, Vogt VM: The retroviral capsid domain dictates virion size, morphology, and coassembly of gag into virus-like particles. J Virol 2005, 79:13463-13472. 9. Pornillos O, Ganser-Pornillos BK, Yeager M: Atomic-level modelling of the HIV capsid. Nature 2011, 469:424-427. 10. von Schwedler UK, Stray KM, G.