Odies in relevant in vitro models. Coupling immune-isolation to pre-analytical protocols for urine processing and sample high quality testing enables detection of a panel of endometrial genes in urine-recovered EVs. Summary/Conclusion: All round, the study delivers a tool for non-invasive monitoring with the functional status on the endometrium, supporting biomedical niches such as assisted fertilization and diagnosis of endometriosis. Funding: ENDEvor POR Region Tuscany (identification on the project) and Exosomics R D ProgrammePF09.Unveiling autologous blood doping: comparative analysis of various purification techniques for urinary extracellular vesicles pioneering miRNA biomarker study Veronika Mussacka, Georg Wittmannb and Michael Pfafflc TUM School of Life Sciences Weihenstephan, Division of Animal Physiology and Immunology, Freising, Germany, Freising, Germany; bDepartment for Transfusion Medicine, Cell therapeutics and Haemostaseology, CD150 Proteins medchemexpress University Hospital LMU, Munich, Germany, M chen, Germany; CD119 Proteins Recombinant Proteins cAnimal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany, Freising, Germanyafive distinctive isolation tactics had been evaluated: ultracentrifugation, membrane affinity, spin column chromatography, immunoaffinity and precipitation. Soon after EV characterization by nanoparticle tracking evaluation, western blotting, and transmission electron microscopy, total RNA was isolated along with a library for smaller RNA sequencing was prepared. The resultant prosperous strategy was then applied to all of the collected samples which were equally analysed concerning their EV distribution and miRNA content. Final results: The comparative analysis disclosed big discrepancies with respect to EV yield, population, and purity, also as RNA yield and detected miRNAs. By applying the top performing approach, which was depending on immunoaffinity, drastically higher amounts of urinary EVs and numerous substantially differentially regulated miRNAs had been observed immediately after ABD. Summary/Conclusion: Urinary EVs and their miRNA profile hold indeed promising attempts for the clear separation of ABD and non-doped athletes. Moreover, the incorporated complicated comparative methodological analysis contributes enormously to future standardization and comparability of urinary EV analysis. Funding: The existing project has been financially supported by the WADA.PF09.Extracellular vesicles as graft biomarkers to address lung transplantation outcome Mario Barilania, Ilaria Righib, Giuseppe Buonoc, Lorenzo Rossod, Mario Nosottie and Lorenza Lazzarica Unit of Regenerative Medicine Cell Factory, Department of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy; Universitdegli Studi di Milano, Milan, Italy; bThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; cUnit of Regenerative Medicine Cell Factory, Division of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; dUniversitdegli Studi di Milano, Milano (MI), Italy, Milano, ItalyIntroduction: Autologous blood doping (ABD) increases the oxygen capacity by way of re-infusion of a person’s autologous red blood cells. It really is as a result employed by endurance athletes having a high level of unreported instances, specially because dependable approaches for unequivocal detection are nonetheless lacking. To su.