Sessed for size (nanoparticle tracking analysis), morphology (transmission electron microscopy) and expression of canonical protein markers CD63, Hsp70, Flo-1 and TSG101 (Western). AFSC-EV RNA was isolated making use of SeraMir, constructed into libraries (CleanTag Smaller RNA) and sequenced on NextSeqJOURNAL OF EXTRACELLULAR VESICLESHigh Output single-end sequencing run. TargetScan was utilized to determine species-specific and evolutionarily conserved miRNA making use of seed sequences across all three species. Pathway enrichment evaluation was carried out working with miR-path. Final results: All round, information on AFSC-EVs from three species (n = two human, n = 2 mouse, n = 1 rat) were included. Four miRNAs (miR-21, miR-24, miR-100 and miR145) had been located in AFSC-EVs from all three species and were reported to exert helpful effects on lung, muscle and kidney regeneration. These miRNAs had been enriched in signalling pathways that involve TGF- (p = 0.004) and TNF- (p = 0.03) as well as the maintenance of stem cell pluripotency (p = 0.0001). We also observed species-specific miRNAs (n = 15 human, n = 6 mouse, n = six rat) contained in AFSC-EVs. VEGFR3/Flt-4 Storage & Stability Summary/Conclusion: AFSC-EVs isolated from different species have some miRNAs which can be shared and evolutionarily conserved. These miRNAs could have a distinct part inside the regenerative effects that AFSC-EVs exert in distinctive diseases. Funding: CIHR-SickKids FoundationPF11.Extra-cellular vesicles in human platelet lysates for clinical use and human cell in vitro propagation Liling Delilaa, Yu-Wen Wua, Ming-Li Choub, David Devosc and Thierry Burnoufda College of Biomedical Engineering, Taipei Health-related University, Taipei, Taiwan (Republic of China); bCentre de Recherche Saint-Antoine (CRSA) INSERM UMRS 938, Taoyuan, Taiwan (Republic of China); cPharmacologie M icale Neurologie, University of Lille, University hospital center, INSERM U-1171, Lille, France; dCollege of Biomedical Engineering, Taipei Healthcare University, Taipei City, Taiwan (Republic of China)along with the size distribution were determined by dynamic light scattering (DLS), nanoparticle tracking evaluation (NTA) and transmission electron microscopy (TEM). EVs functional activity was assessed for the expression of tissue element and phosphatidylserine (PS) activity. Also, the HPLs have been tested for their thrombin and plasmin activity, anti-oxidative property and thrombin generation capacity Benefits: Abundant number of EVs (1010 1012/mL) was identified in all HPLs fractions. DLS analysis showed that isolated EVs in PPL, HPPL, SCPL and HSCPL have size distribution approximately ranging as follows: one hundred 250 nm; 45 210 nm; 145 230 nm and 55 180 nm, respectively, these information being confirmed by NTA and TEM. None with the HPLs have been found to have detectable TF-expressing EVs but some important differences in PS-expressing EVs, at the same time as thrombin, plasmin and anti-oxidative activity had been found, possibly linked to their EVs composition Summary/Conclusion: This study establishes that all HPLs evaluated have a high content material of EVs. Variations in functional activity have been also unveiled supporting the will need for additional studies with the 5-HT1 Receptor Antagonist site physiological functions of HPL-derived EVs in cell-based and preclinical animal modelsPF11.EV-mediated delivery of enzymatically fabricated size-controllable functional DNA/RNA microstructures for therapeutic applications Hyejin Kim, Dajeong Kim and Jong Bum Lee Division of Chemical Engineering, University of Seoul, Seoul, Republic of KoreaIntroduction: Human platelet lysates (H.