s. uniprot.org/uniprot/Q6WUC1 Involved in transport of water along with other compact neutral mol ecules across cellular biological membranes (Kapilan et al. 2018) Catalyses the very first committed step of fatty acid synthesis, the carboxylation of acetylCoA to malonylCoA (Sasaki and Nagano 2004) Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in sesquiterpene (C15) olefins biosynthesis uniprot.org/uniprot/O81086 Involved in gibberellin signal ling (Sun 2011) Mediates the amino acid proline and glycine betaine transport uniprot. org/uniprot/P(2022) 23:NZPradTrx049513_CNZPradTrx049513_CPutative prolinerich arabinoga lactan eIF4 custom synthesis protein 4e+NZPradTrx079868_CPREDICTED: (RS)norcoclaurine 6OmethyltransferaselikebNZPradTrx054832_CAquaporinlike proteink+NZPradTrx069597_CAcetylCoA carboxylase BCCP subunitaNZPradTrx117954_CEalphabisabolene synthaseb+NZPradTrx087252_CTPA: putative GID1like gibberel lin receptorg+ +(two) Web page 20 ofNZPradTrx074370_CPutative proline transporterkNZPradTrx112166_CNantongo et al. BMC Genomics(2022) 23:Table 5 (continued)Function Related with metal ion binding and oxidoreductase activity uniprot.org/unipr ot/Q9LIG0 T7-B-MJ T7-B-S T7-N-MJ T14-B-MJ T14-B-S T14-N-MJ T21-B-MJ T21-B-S T21-N-MJ +Scion transcript codeGene nameNZPradTrx113904_C06/ NZPradTrx101343_CPREDICTED: clavaminate syn thaselike protein At3g21360 likejaprimary metabolismbsecondary metabolismcdigestive inhibitorsdpathogenesis-related (PR) protein familiesegenes involved with physical strengthening with the cell-wallftranscription factorsgphytohormones and signalling moleculeshgeneral catalystsimolecules involved in transcriptionjmolecules involved in broad biotic and abiotic anxiety responseskbroad function genesPage 21 ofNantongo et al. BMC Genomics(2022) 23:Page 22 ofFig. five An upset plot showing the number of exclusive and overlapping differentially expressed transcripts following methyl jasmonate (MJ) and also the bark stripping (strip) treatment options over time and plant parts (Dopamine Receptor Species needles [N] and bark [B]). T7, T14 and T21 referred to sampling undertaken 7, 14 and 21 days just after therapy respectively. As an instance, 749 transcripts within the needles (N) have been differentially expressed uniquely at T7 following MJ remedy and were not differentially expressed at any time point inside the bark (B) or other time point in the needles (N). Treatments or instances where overlapping transcripts happen are linked by lines. By way of example, the most popular overlapping transcripts were the 227 that had been differentially expressed only within the needles at T7 and T14 in methyl jasmonate (MJ) treatment. The other transcript combinations are ordered by their frequency of occurrence according to the several unique or overlapping combinations in which they were differentially expressed within the methyl jasmonate (MJ) and bark stripping (strip) treatments at every single time. Note that no transcripts have been differentially expressed inside the needles following bark stripping at any timesignificant down-regulation at T7 in each strip and MJ treated samples.Functional classification of differentially expressed transcriptsTo assess the overall effect on the treatment options across various gene households and molecular processes, the GO terms had been determined for the up-regulated and downregulated transcripts for every single situation (time remedy plant aspect). There was an overall similarity within the GO terms for genes that had been up- and down-regulated in the strip and methyl jasmonate therapies.