Product Name :
Anti-DYKDDDDK tag, Mouse IgG2a antibody
Applications:
WB,ICC/IF,ELISA,IP,Flow Cyt
Reactivity :
DYKDDDDK tag
Conjugate:
Unconjugated
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-DYKDDDDK tag, Mouse IgG2a antibody is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, Mouse IgG2a antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2a Fc. Based on western blot and ELISA, Anti-DYKDDDDK tag, Mouse IgG2a antibody reacts with the DYKDDDDK tag selectively, no reactivity with other proteins. | Immunogen: DYKDDDDK tag fusion protein | Host: Alpaca pacous | Isotype: Fab of alpaca IgG1 fused to Mouse IgG2a Fc(mutation) | Conjugate: Unconjugated | Specificity: DYKDDDDK tag sequence | Cross-Reactivity: Highly selective for DYKDDDDK tag sequence | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.{{2055200-88-7} web|{2055200-88-7} Protocol|{2055200-88-7} Formula|{2055200-88-7} custom synthesis} 5), 0.{{779353-01-4} web|{779353-01-4} Purity & Documentation|{779353-01-4} Formula|{779353-01-4} supplier} 05% sucrose, 0.PMID:28846281 1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C | Background:The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-DYKDDDDK tag, Mouse IgG2a antibody is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, Mouse IgG2a antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2a Fc. Based on western blot and ELISA, Anti-DYKDDDDK tag, Mouse IgG2a antibody reacts with the DYKDDDDK tag selectively, no reactivity with other proteins.
Immunogen:
DYKDDDDK tag fusion protein
Host :
Alpaca pacous
Isotype:
Fab of alpaca IgG1 fused to Mouse IgG2a Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Function:
WB: 1:5,000-1:20,000ELISA: 1:5,000-1:20,000ICC/IF: 1:200-1:1000IP: 1-2ug/sampleFlow Cyt：1µg for 106 cellsDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.