Product Name :
Anti-HER2, Human antibody
Applications:
ELISA,Flow Cyt
Reactivity :
Human/Cynomolgus
Conjugate:
Unconjugated
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-HER2, Human antibody is designed for detecting human HER2 specifically. Anti-HER2, Human antibody is recombinant VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc. Based on ELISA, Anti-HER2, Human antibody reacts with human HER2, and has reactivity with cynomolgus HER2. | Immunogen: Recombinant human HER2 | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation) | Conjugate: Unconjugated | Specificity: Human HER2 | Cross-Reactivity: Cross-reactivity with cynomolgus HER2 | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C | Background:HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.{{1644670-37-0} MedChemExpress|{1644670-37-0} Protocol|{1644670-37-0} Description|{1644670-37-0} manufacturer} Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-HER2, Human antibody is designed for detecting human HER2 specifically. Anti-HER2, Human antibody is recombinant VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc. Based on ELISA, Anti-HER2, Human antibody reacts with human HER2, and has reactivity with cynomolgus HER2.
Immunogen:
Recombinant human HER2
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Function:
ELISA: 1:4,000-1:10000Flow Cytometry：1:200-1:1000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc.{{641571-10-0} web|{641571-10-0} Protocol|{641571-10-0} Data Sheet|{641571-10-0} custom synthesis} The actual dilution used must be determined empirically.PMID:30285389