Us glucose (0.5 ), or glucose (4 ). Culture samples were harvested, concentrated to an
Us glucose (0.5 ), or glucose (4 ). Culture samples were harvested, concentrated to an OD600 nm of 30, and the suspension (1 mL) was quenched with 4.5 mL of 25 mM formic acid in acetonitrile at ?0 . The samples were incubated for 15 min on ice with occasional mixing, chilled with liquid nitrogen, and lyophilized. Samples were dissolved in 150 L ammonium formate buffer (50 mM, pH 3.5, 2 BMS-986020MedChemExpress BMS-986020 methanol). After mixing, samples were centrifuged twice (20,000 ?g, 5 min, 4 ) and the supernatant was used for analysis. LC-MS analyses were performed with a Rheos 2200 HPLC system (Flux Instruments, Basel, Switzerland) coupled to an LTQ Orbitrap mass spectrometer (Thermo Fisher PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27527552 Scientific, Waltham, MA, USA), equipped with an electrospray ionization probe. Coenzyme A esters were analyzed as described previously [98] with slight modifications [99]. First, potential CoA esters were identified applying a general approach as described previously [99]. In a second identification approach, samples were spiked with uniformly 13C labeled cell extract from Methylobacterium extorquens AM1 as internal standard. CoA esters of M. extorquens have been extensively characterized previously [98].Nucleotide sequence accession numberThe complete genome sequence of Arthrobacter sp. Rue61a was deposited in GenBank under the accession numbers [GenBank:CP003203] Genbank:CP003205].Additional filesAdditional file 1: Table S1. Genes of Arthrobactersp. Rue61as associated with putative genomic islands. Additional file 2: Tables S2. Putative transporters and binding proteins of Arthrobactersp. Rue61a.Niewerth et al. BMC Genomics 2012, 13:534 http://www.biomedcentral.com/1471-2164/13/Page 18 of6.7.8.9.10.11.12. 13. 14. 15. 16.17.18.19.20.21.22. 23.24.25. 26.27.28.Hanbo Z, Changqun D, Qiyong S, Weimin R, Tao S, Lizhong C, Zhiwei Z, Bin H: Genetic and physiological diversity of phylogenetically and geographically distinct groups of Arthrobacter isolated from lead-zinc mine tailings. FEMS Microbiol Ecol 2004, 49:333?41. Macur RE, Jackson CR, Botero LM, McDermott TR, Inskeep WP: Bacterial populations associated with the oxidation and reduction of arsenic in an unsaturated soil. Environ Sci Technol 2004, 38:104?11. Jareonmit P, Sajjaphan K, Sadowsky MJ: Structure and diversity of arsenicresistant bacteria in an old tin mine area of Thailand. J Microbiol Biotechnol 2010, 20:169?78. Zhang WH, Huang Z, He LY, Sheng XF: Assessment of bacterial communities and characterization of lead-resistant bacteria in the rhizosphere soils of metal-tolerant Chenopodium ambrosioides grown on lead-zinc mine tailings. Chemosphere 2012, 87:1171?178. Bieszkiewicz E, Boszczyk-Maleszak H, Kaczorowska B, Mycielski R: Isolation and identification of bacteria from activated sludge purifying petroleum wastewaters. Acta Microbiol Pol 1995, 44:171?79. K pfer P, Erhart R, Beimfohr C, B ringer J, Wagner M, Amann R: Characterization of bacterial communities from activated sludge: culturedependent numerical identification versus in situ identification using group- and genus-specific rRNA-targeted oligonucleotide probes. Microb Ecol 1996, 32:101?21. Juang DF, Hwu CS: Agar-plated bacteria found in the activated sludge of lab-scale SBR and CFSTR systems. Bioresour Technol 2003, 89:75?9. Boylen CW: Survival of Arthrobacter crystallopoietes during prolonged periods of extreme desiccation. J Bacteriol 1973, 113:33?7. Hagedorn C, Holt JG: A nutritional and taxonomic survey of Arthrobacter soil isolates. Can J Microbiol 1975, 21.